A5053395152- Prostate Cancer Treatment and Research
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Renal and related cancers
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Metabolism, Diabetes, and Cancer
- Biomedical Ethics and Regulation
- Congenital heart defects research
- Pregnancy and preeclampsia studies
- Histone Deacetylase Inhibitors Research
- Prostate Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Chemokine receptors and signaling
- Protease and Inhibitor Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Cancer Mechanisms and Therapy
- Connective Tissue Growth Factor Research
- Retinoids in leukemia and cellular processes
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
American University of Beirut
2014-2024
Lebanese University
2015-2016
Aix-Marseille Université
2012-2013
Inserm
2009-2013
Institut de Neurobiologie de la Méditerranée
2010
Cancer Stem Cells (CSCs) are a sub-population of cells, identified in most tumors, responsible for the initiation, recurrence, metastatic potential, and resistance different malignancies. In prostate cancer (PCa), CSCs were thought to be generation lethal subtype, commonly known as Castration-Resistant Prostate (CRPC). vitro models investigate properties PCa highly required. Sphere-formation assay is an method used identify study their properties. Here, we report detailed methodology on how...
// Wei-Yu Chen 1, 2, * , Shih-Yang Liu 3, Yung-Sheng Chang 4 Juan Yin 5 Hsiu-lien Yeh 6 Tarek H. Mouhieddine 7 Ola Hadadeh Wassim Abou-Kheir Yen-Nien 1 Department of Pathology, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan 2 School Medicine, College 3 Acupuncture and Manipulation, International Education, Tianjin University Traditional Chinese Tianjin, China Graduate Institute Cancer Biology Drug Discovery, Science Technology, Cell Branch, National Institute, Institutes...
BACKGROUND: Prostate cancer (PCa) is the second most frequent cause of cancer-related death in men worldwide. It a heterogeneous disease at molecular and clinical levels which makes its prognosis treatment outcome hard to predict. The epithelial-to-mesenchymal transition (EMT) marks key step invasion malignant progression PCa. We sought assess co-expression epithelial cytokeratin 8 (CK8) mesenchymal vimentin (Vim) locally-advanced PCa as indicators EMT consequently predictors status disease....
Embryonic stem cells (ESCs) differentiate in vivo and vitro into all cell lineages, they have been proposed as cellular therapy for human diseases. However, the molecular mechanisms controlling ESC commitment toward specific lineages need to be specified. We previously found that p38 mitogen-activated protein kinase (p38MAPK) pathway inhibits neurogenesis is necessary mesodermal formation during critical first 5 days of mouse commitment. This period corresponds expression master genes direct...
Three‑dimensional (3D) organoid culture systems are emerging as potential reliable tools to investigate basic developmental processes of human disease, especially cancer. The present study used established and modified conditions report successful generation characterization patient‑derived organoids from fresh primary tissue specimens patients with treatment‑naïve prostate cancer (PCa). Fresh were collected, digested enzymatically the resulting cell suspensions plated in a 3D environment...
Abstract Retinoids are vitamin A derivatives that regulate crucial biological processes such as cellular proliferation, apoptosis, and differentiation. The use of natural retinoids in cancer therapy is limited due to their toxicity the acquired resistance by cells. Therefore, synthetic were developed, atypical adamantyl retinoid ST1926 provides enhanced bioavailability reduced toxicity. We have assessed vitro vivo antitumor properties mechanism action targeting stem‐like cells population...
Embryonic stem (ES) cells differentiate in vitro into all cell lineages. We previously found that the p38 mitogen activated kinase (p38MAPK) pathway controls commitment of ES toward either cardiomyogenesis (p38 on) or neurogenesis off ). In this study, we show p38α knock-out do not cardiac, endothelial, smooth muscle, and skeletal muscle Reexpression p38MAPK these partially rescues their mesodermal differentiation defects corrects high level spontaneous cells. Wild-type were treated with a...
Prostate cancer (PCa) is the second leading cause of cancer-related mortality and morbidity among males worldwide. Deciphering biological mechanisms molecular pathways involved in PCa pathogenesis progression has been hindered by numerous technical limitations mainly attributed to limited number cell lines available, which do not recapitulate diverse phenotypes clinical disease. Indeed, proven problematic establish as culture due its heterogeneity remains a challenge, despite various vitro...
Regulation of the extracellular matrix (ECM) plays an important functional role either in physiological or pathological conditions. The plasminogen activation (PA) system, comprising uPA and tPA proteases their inhibitor PAI-1, is one main suppliers proteolytic activity contributing to tissue remodeling. Although its function development well documented, precise mouse embryonic stem cell (ESC) differentiation vitro unknown. We found that PA system components are expressed at very low levels...
CCN1 is an extracellular matrix-associated protein thought to be implicated in tumor-stromal interaction several solid tumors. The aim of our pilot study was evaluate the correlation between expression stromal cells, pancreatic intraepithelial neoplasia (PanIN) and ductal adenocarcinoma cells resected (PDAC) specimens, correlate that clinically.A total 42 paraffin-embedded PDAC tumor specimens were stained for evaluated via immunohistochemical (IHC) analysis. Statistical analysis performed...
Embryonic stem cells (ESCs) serve as a crucial
Cardiac Progenitor CellsThe heart is the first organ to become fully functional during development in all organisms.Despite numerous studies model organisms last two decades, identity of cardiac progenitor cell (CPC) remains unclear.However, have confirmed that most cells originate from mesoderm.Work recent years has shed light on a few genetic markers are believed be candidates for expression illusive cell, such as NKX 2.5, ISL-1, FLK-1, MESP1 and others [1][2][3][4].In mouse embryonic...
Introduction The currently available cancer models fail to recapitulate the heterogeneity of Prostate (PCa), its metastasis, and progression castration-resistant states. Many drug candidates that succeed in preclinical deliver a good outcome clinical trials, resulting ineffective patient treatment misused resources. In this aspect, development three-dimensional (3D) organoid culture systems render it feasible recap convolution organogenesis vitro, promoting generation novel more...
Abstract Several attempts have been made to understand the exact mechanisms underlying pathogenesis of prostate cancer (PC); however, currently available models fail recapitulate heterogeneity this tumor, its metastasis, and progression castration-resistant states. Moreover, many drug candidates that succeed in preclinical deliver a good outcome clinical trials, resulting ineffective patient treatment misused resources. In aspect, development three-dimensional (3D) organoid culture systems...
Abstract Background: Prostate Cancer (PCa) is a heterogeneous disease. Currently available in vitro and vivo cancer models fail to recapitulate the spectrum of clinical biological challenges; accurate for translational research remain significant unmet need. Objective: The aim this work employ fresh tissue specimens from treatment-naïve cohort patients with prostate optimize establish 3D patient-derived organoids as an disease model PCa progression drug response. In addition, acknowledging...