A5049770225- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Lipids, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Neuroblastoma Research and Treatments
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Cancer therapeutics and mechanisms
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Nigella sativa pharmacological applications
- Pregnancy and preeclampsia studies
- Anesthesia and Neurotoxicity Research
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Cellular Mechanics and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Prostate Cancer Diagnosis and Treatment
- Bioactive Compounds and Antitumor Agents
- Glioma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
American University of Beirut
2016-2025
American University of Beirut Medical Center
2015-2021
Mount Sinai Medical Center
2021
Faculty (United Kingdom)
2019
Lebanese University
2016
Center for Cancer Research
2011-2015
National Cancer Institute
2010-2012
National Institutes of Health
2010-2012
American University
2012
ORCID
2010
Cancer Stem Cells (CSCs) are a sub-population of cells, identified in most tumors, responsible for the initiation, recurrence, metastatic potential, and resistance different malignancies. In prostate cancer (PCa), CSCs were thought to be generation lethal subtype, commonly known as Castration-Resistant Prostate (CRPC). vitro models investigate properties PCa highly required. Sphere-formation assay is an method used identify study their properties. Here, we report detailed methodology on how...
Thymoquinone (TQ), the most abundant constituent in black seed, was shown to possess potent chemopreventive activities against DMBA-initiated TPA-promoted skin tumors mice. Despite potential interest TQ as a antineoplastic agent, its mechanism of action has not been examined yet. Using primary mouse keratinocytes, papilloma (SP-1) and spindle (I7) carcinoma cells, we studied cellular molecular events involved TQ's activity. We show that non-cytotoxic concentrations reduce proliferation...
Epithelial-mesenchymal transition (EMT) is implicated in various pathological processes within the prostate, including benign prostate hyperplasia (BPH) and cancer progression. However, an ordered sequence of signaling events initiating carcinoma-associated EMT has not been established. In a model transforming growth factor β (TGFβ)-induced prostatic EMT, SLUG dominant regulator initiation vitro vivo, as demonstrated by inhibition following Slug depletion. contrast, SNAIL depletion was...
// Wei-Yu Chen 1, 2, * , Shih-Yang Liu 3, Yung-Sheng Chang 4 Juan Yin 5 Hsiu-lien Yeh 6 Tarek H. Mouhieddine 7 Ola Hadadeh Wassim Abou-Kheir Yen-Nien 1 Department of Pathology, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan 2 School Medicine, College 3 Acupuncture and Manipulation, International Education, Tianjin University Traditional Chinese Tianjin, China Graduate Institute Cancer Biology Drug Discovery, Science Technology, Cell Branch, National Institute, Institutes...
Prostate cancer (PCa) is one of the most commonly diagnosed cancers among men worldwide. Despite presence accumulated clinical strategies for PCa management, limited prognostic/sensitive biomarkers are available to follow up on disease occurrence and progression. MicroRNAs (miRNAs) small non-coding RNAs that control gene expression through post-transcriptional regulation their complementary target messenger RNA (mRNA). MiRNAs modulate fundamental biological processes play crucial roles in...
Glucagon-like peptide-1 (GLP-1), an incretin hormone renowned for its role in post-meal blood sugar regulation and glucose-dependent insulin secretion, has gained attention as a novel treatment diabetes through GLP-1 receptor agonists (GLP-1-RA). Despite their efficacy, concerns have been raised regarding the potential associations between GLP-1-RA certain malignancies, including medullary thyroid cancer. However, evidence of association with prostate cancer (PCa) remains inconclusive. This...
Podosomes, adhesion structures capable of matrix degradation, have been linked with the ability cells to perform chemotaxis and invade tissues. Wiskott-Aldrich Syndrome protein (WASp), an effector RhoGTPase Cdc42 a Src family kinase substrate, regulates macrophage podosome formation. In this study, we demonstrate that WASp is active in podosomes by using TIRF-FRET microscopy. Pharmacological RNA interference approaches suggested continuous activity required for formation function. Rescue...
Wiskott-Aldrich syndrome protein (WASP)-family verprolin homologous (WAVE) proteins play a major role in Rac-induced actin dynamics, but Rac does not bind directly to WAVE proteins. It has been proposed that either the insulin receptor substrate 53 (IRSp53) or complex of containing Abelson interactor 1 (Abi1) mediates interaction WAVE2 and Rac. Depletion endogenous IRSp53 by RNA-mediated interference (RNAi) RAW/LR5 macrophage cell line resulted significant reduction Rac1Q61L-induced surface...
// Man Kit Siu 1,2,* , Wassim Abou-Kheir 3,* Juan Yin 4,* Yung-Sheng Chang 1 Ben Barrett 4 Florent Suau Orla Casey Wei-Yu Chen 5 Lei Fang Paul Hynes Yao-Yu Hsieh 1,6,9 Yen-Nien Liu 1,7 Jiaoti Huang 8 and Kathleen Kelly Graduate Institute of Cancer Biology Drug Discovery, College Medical Science Technology, Taipei University, Taipei, Taiwan 2 Department Anesthesiology, Wan Hospital, 3 Anatomy, Cell Physiological Sciences Faculty Medicine, American University Beirut, Lebanon Branch, National...
Nervous system tumors represent some of the highly aggressive cancers in both children and adults, particularly neuroblastoma glioblastoma. Many studies focused on pathogenic role Akt pathway mechanistic target Rapamycin (mTOR) complex mediating progression various types cancer, which designates Akt/mTOR signaling as a master regulator for cancer. Current are also elucidating mechanisms cancer stem cells (CSCs) replenishing explicating strong correlation between CSC biology. This instigates...
Primary prostate cancer almost always has a luminal phenotype. However, little is known about the stem/progenitor properties of transformed cells within tumors. Using aggressive Pten/Tp53-null mouse model cancer, we show that two classes progenitors exist tumor. Not only did tumors contain previously described multipotent progenitors, but also major population committed progenitors. Luminal cells, sorted directly from or grown as organoids, initiated adenocarcinoma multilineage histological...