Cancer-associated fibroblasts (CAFs) are essential for tumor microenvironment remodeling and correlate with progression. However, interactions between CAFs cells immune in triple-negative breast cancer (TNBC) still poorly explored. Here, we investigate the role of TNBC potential novel mediators their functions. The clustering classic markers was applied to estimate relative abundance cohorts. Primary were isolated from normal samples. RNA culture medium subjected sequencing mass spectrometry...

It was unclear whether breast cancer subtypes are associated with the risk of site-specific metastases. This study aimed to evaluate relationship between molecular and distant metastatic sites their prognostic significance.We identified 295,213 patients invasive from 2010 2014 using Surveillance, Epidemiology End Results database. Subtypes were classified into four categories: hormone receptor (HR+)/human epidermal growth factor 2 (HER2-), HR+/HER2+, HR-/HER2+, triple-negative (HR-/HER2-)....

Circular RNA CDR1as/ciRS-7 functions as an oncogenic regulator in various cancers. However, there has been a lack of systematic and comprehensive analysis to further elucidate its underlying role cancer. In the current study, we firstly performed bioinformatics CDR1as among 868 cancer samples by using RNA-seq datasets MiOncoCirc database. Gene ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), gene set enrichment (GSEA), CIBERSORT, Estimating Proportion Immune Cancer cells (EPIC),...

Abstract Background Triple-negative breast cancer (TNBC) is the most malignant subtype of with highly invasive ability and metastatic nature to lymph nodes. Long non-coding RNAs (lncRNAs) have been widely explored in tumorigenesis progression. However, their roles TNBC node metastasis remains rarely studied. Methods The expression lncRNA upregulated (HUMT) cell lines tissues was detected by quantitative real-time PCR (qRT-PCR) situ hybridization (ISH). RNA immunoprecipitation (RIP) pulldown...

Osteoporosis (OP) is one of the most common diseases in elderly, and it not effectively solved by current treatments. Mesenchymal stem cells (MSCs) have multiple differentiation potentials, which can induce osteogenic to treat OP; however, important understand how remotely control detect vivo real time. Here, we developed an upconversion nanoparticle (UCNP)-based photoresponsive nanoplatform for near-infrared (NIR) light-mediated intracellular icariin (ICA) release regulate MSCs OP therapy....

Abstract Introduction Cancer‐associated fibroblasts (CAFs) are correlated with the immunotherapy response. However, culprits that link CAFs to resistance still rarely investigated in real‐world studies. Objectives This study aims systematically assess landscape of cancer patients by combining single‐cell and bulk profiling data from pan‐cancer cohorts. We further sought decipher expression, survival predictive value association response biglycan (BGN), a proteoglycan extracellular matrix,...

Abstract Distant metastasis remains the primary cause of morbidity in patients with breast cancer. Hence, development more efficacious strategies and exploration potential targets for metastatic cancer are urgently needed. The data six brain metastases (BCBrM) from two centres were collected, a comprehensive landscape entire tumour ecosystem was generated through utilisation single‐cell RNA sequencing. We utilised Monocle2 CellChat algorithms to investigate interrelationships among each...

Abstract MicroRNAs (miRNAs) are small non-protein-codingRNAs that function as negative gene expression regulators. miRNA-210 (miR-210) has recently been recognized in the pathogenesis of osteonecrosis associated with angiogenesis. Herein we aimed to explore clinical significance miR-210 treatment for postmenopausal osteoporosis. The was detected bone marrow mesenchymal stem cells (BMSCs) vitro and significantly promoted vascular edothelial growth factor (VEGF) BMSCs a time-dependent manner (...

As a new rising star of non-coding RNA, circular RNAs (circRNAs) emerged as vital regulators with biological functions in diverse cancers. However, the function and precise mechanism vast majority circRNAs triple-negative breast cancer (TNBC) occurrence progression have not been clearly elucidated. In current study, we identified further investigated hsa_circ_0002453 (circRAD18) by analyzing our previous microarray profiling. Expression circRAD18 was found significantly upregulated TNBC...

The interaction between hypoxia and immune status has been confirmed in various cancer settings, corresponding treatments have investigated. However, reliable biomarkers are needed for individual treatment, so we sought to develop a novel scoring system based on status. Prognostic hypoxia-immune status-related signatures of patients with triple-negative breast (TNBC) were identified Cancer Genome Atlas (TCGA) (N = 158), Molecular Taxonomy Breast International Consortium (METABRIC) 297),...

Background: There is increasing evidence that circular RNAs (circRNAs) participate in regulating cancer progression.However, the function and potential molecular mechanisms of circRNA triple negative breast (TNBC) are currently largely unclear.Results: We found circAHNAK1 was significantly down-regulated TNBC, its expression negatively associated with RFS OS.Overexpression can inhibit TNBC proliferation, migration invasion vitro.In vivo studies confirmed inhibited tumor growth...

Osteoporosis is one of the common orthopedic diseases featured in low bone mineral density. Exosomes have been proven to be potential markers for many and health problems. The roles messenger RNAs microRNAs osteoporosis comprehensively studied; however, little research has focused on function plasma exosomal transfer RNA-derived fragments (tRFs) osteoporosis.We collected samples from 40 healthy controls patients, all exosomes were isolated with combined centrifugation characterized by...

Abstract CircRNAs (circular RNA) are reported to regulate onset and progress multiple cancers. Nonetheless, the function along with underlying mechanisms of circRNAs in HER‐2‐positive breast cancer (BC) remains unclear. CircRNA microarrays were performed elucidate expression profiles BC cells. circRNA levels quantified using qRT‐PCR assay. Various vitro vivo assays employed further explore effects circGFRA1 interactions circGFRA1, miR‐1228 AIFM2 Her‐2‐positive BC. CircGFRA1 was remarkably...

As an intriguing class of RNA, circular RNAs (circRNAs) are vital mediators various diseases including cancers. However, the biological role and underlying mechanism majority circRNAs still ambiguous in progression triple-negative breast cancer (TNBC). In this study, we characterized further investigated hsa_circ_0009362 (circGNB1) by reanalyzing circRNA microarray profiling our previous study. Validating qRT-PCR, circGNB1 was overexpressed TNBC cell lines high expression associated with...

Our study aims to construct a prognosis-related immune phenotype classifier for predicting clinical prognosis and activity in triple-negative breast cancer (TNBC). A total of 237 patients with TNBC from Sun Yat-sen University Cancer Center (SYSUCC) 533 public datasets were included our study. stromal quantified index was generated LASSO Cox regression model based on five cells evaluated by CIBERSORT or IHC used determine phenotypes. Immune features the samples before chemotherapy. 119 SYSUCC...

Abstract Background Previous studies have indicated that quantitative MRI (qMR) is beneficial for diagnosis of breast cancer. As a novel qMR technology, synthetic (syMRI) may be advantageous by offering simultaneous generation T1 and T2 mapping in one scan within few minutes without concern to the deposition gadolinium contrast agent cell nucleus. In this study, potential derived from Synthetic (SyMRI) diagnose cancer was investigated. Methods From April 2018 May 2019, total 87 patients with...

The prognosis of breast cancer brain metastasis (BCBM) is extremely poor due to its resistance conventional therapy. Elucidation the molecular mechanisms BCBM could contribute development new therapeutic targets. In this study, we isolated RNA samples from primary or BCBM, and then performed mRNA profiling. We determined that SOX2 associated with occurrence be a predictor BCBM. High levels were significantly decreasing BCBM-free survival in patients. Overexpression cells enhanced cell...

Background It is of importance to minimize ischemia reperfusion (I/R) injury during liver operations. Reducing the inflammatory reaction an effective way achieve this goal. Notably, adiponectin (APN) was found have anti-inflammatory activity in heart and renal I/R injury. Herein, we investigated role APN Methods Wistar rats were randomized four groups: (1) sham group; (2) control (3) I/R+APN (4) I/R+APN+AMPK inhibitor group. Liver blood samples collected 6h 24h after reperfusion. function...

Melatonin has been recognized to slow breast cancer growth. The molecular mechanisms may involve long non-coding RNAs (lncRNAs). However, little is known on how melatonin affects lncRNA expression and function in cancer. We used microarrays explore the profile of mRNAs lncRNAs melatonin-treated cells. Kyoto encyclopedia genes genomes (KEGG) Reactome pathways analysis were performed identify signaling affected by altered expressed after treatment. To functions selected differentially mRNA...

Abstract Melatonin has been reported to have tumor-suppressive effects via comprehensive molecular mechanisms, and long non-coding RNAs (lncRNAs) may participate in this process. However, the mechanism by which melatonin affects function of lncRNAs triple-negative breast cancer (TNBC), most aggressive subtype cancer, is still unknown. Therefore, we aimed investigate differentially expressed mRNAs melatonin-treated TNBC cells interaction mechanisms. Microarray analyses were performed identify...

Background As one of the most common malignancies worldwide, breast cancer (BC) exhibits high heterogeneity molecular phenotypes. The evolving view regarding DNA damage repair (DDR) is that it context-specific and heterogeneous, but its role in BC remains unclear. Methods Multi-dimensional data transcriptomics, genomics, single-cell transcriptome profiling were obtained to characterize DDR-related features BC. We collected 276 genes based on Molecular Signature Database (MSigDB) database...