A5101636639- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- CAR-T cell therapy research
- Cutaneous Melanoma Detection and Management
- Virus-based gene therapy research
- Melanoma and MAPK Pathways
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Cancer Immunotherapy and Biomarkers
- Traditional Chinese Medicine Analysis
- Nephrotoxicity and Medicinal Plants
- HER2/EGFR in Cancer Research
- Genomics and Phylogenetic Studies
- Cancer Research and Treatments
- Land Use and Ecosystem Services
- Natural product bioactivities and synthesis
- Urban Heat Island Mitigation
- Plant nutrient uptake and metabolism
- Remote Sensing and Land Use
- Microbial Community Ecology and Physiology
- Angiogenesis and VEGF in Cancer
- Drug-Induced Hepatotoxicity and Protection
- Extracellular vesicles in disease
- Trace Elements in Health
Peking University Cancer Hospital
2021-2025
State Administration of Traditional Chinese Medicine of the People's Republic of China
2023-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Peking University
2021-2025
Ministry of Education of the People's Republic of China
2023-2024
Minzu University of China
2021-2023
Northwest Minzu University
2012-2019
Nanjing University
2010-2011
Henan Agricultural University
2009
Xinyang College of Agriculture and Forestry
2007
MYB is the largest plant transcription factor gene family playing vital roles in growth and development. However, it has not been systematically studied Salvia miltiorrhiza, an economically important medicinal plant. Here we report genome-wide identification characterization of 110 R2R3-MYBs, subfamily MYBs S. miltiorrhiza. The domain other motifs SmMYBs are largely conserved with Arabidopsis AtMYBs, whereas divergence AtMYBs also exists, suggesting conservation diversity MYBs. may be...
WRKY proteins comprise a large family of transcription factors and play important regulatory roles in plant development defense response. The gene Salvia miltiorrhiza has not been characterized.A total 61 SmWRKYs were cloned from S. miltiorrhiza. Multiple sequence alignment showed that could be classified into 3 groups 8 subgroups. Sequence features, the domain other motifs are largely conserved with Arabidopsis AtWRKYs. Each group domains contains characteristic sequences, group-specific...
Abstract Salvia miltiorrhiza is well known for its clinical practice in treating heart and cardiovascular diseases. Its roots, used traditional Chinese medicine materials, are usually brick-red due to accumulation of red pigments, such as tanshinone IIA I. Here we report a S. line (shh) with orange roots. Compared the roots normal plants, contents tanshinones single bond at C-15,16 were increased, whereas those double significantly decreased shh. We assembled high-quality chromosome-level...
Aporphine alkaloids are a large group of natural compounds with extensive pharmaceutical application prospects. The biosynthesis aporphine has been paid attentions in the past decades. Here, we determined contents four 1-benzylisoquinoline and five root, stem, leaf, flower Aristolochia contorta Bunge, which belongs to magnoliids. Two CYP80 enzymes were identified characterized from A. contorta. Both them catalyze unusual C-C phenol coupling reactions directly form alkaloid skeleton....
Background OH2 is an oncolytic virus derived from herpes simplex type 2. A phase Ia/Ib clinical trial in China was conducted patients with unresected stage III–IV melanoma, the majority of whom had acral type, to assess safety and preliminary efficacy OH2. Methods The enrolled histologically confirmed unresectable III or advanced IV melanoma. In Ia, nine received single-dose treatment across three dose levels (10 6 , 10 7 8 CCID 50 /mL, where represents cell culture infectious 50%) while six...
SPLs, a family of transcription factors specific to plants, play vital roles in plant growth and development through regulation various physiological biochemical processes. Although Populus trichocarpa is model forest tree, the PtSPL gene has not been systematically studied. Here we report identification 28 full-length PtSPLs, which distribute on 14 P. chromosomes. Based phylogenetic relationships SPLs Arabidopsis, can be classified into 6 groups. Each group contains at least an AtSPL. The...
Abstract SQUAMOSA promoter binding protein‐likes (SPLs) are plant‐specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs Salvia miltiorrhiza (Danshen), a significant medicinal widely used Traditional Chinese medicine (TCM) for >1,700 years an emerging model TCM studies. Through genome‐wide identification subsequent molecular cloning, we identified total 15 SmSPLs with divergent sequence features, gene structures,...
Flavonoids are a class of important secondary metabolites with broad spectrum pharmacological functions. Salvia miltiorrhiza Bunge (Danshen) is well-known traditional Chinese medicinal herb diversity flavonoids. However, flavonoid biosynthetic enzyme genes have not been systematically and comprehensively analyzed in S. miltiorrhiza. Through genome-wide prediction molecular cloning, twenty six biosynthesis-related gene candidates were identified, which novel. They belong to nine families...
Soil is the bearing centre of terrestrial ecosystems. Oil pollution leads to changes in physical and chemical properties soil varying degrees. Polluted soils form a unique microbial species composition, which provides rich materials for bioremediation oil-contaminated through biological enhancement. Understanding composition petroleum-contaminated can provide better method remediation. Based on this, 16 S rRNA ITS genetic markers were used analyse bacterial fungal microbiota soil, their...
Acral melanoma, known for low tumor mutation burden, responds poorly to immunotherapy. A standard therapy is still lacking.
Contamination of soil by petroleum is becoming increasingly serious in the world today. However, research on gene functional characteristics, metabolites and distribution microbial genomes oil-contaminated limited. Considering that, metagenomic metabonomic were used to detect microbes soil, changes pathways analyzed. We found that oil pollution significantly changed composition microorganisms metabolites, promoted relative abundance Pseudoxanthomonas, Pseudomonas, Mycobacterium,...
SUMMARY Prunella vulgaris is one of the bestselling and widely used medicinal herbs. It recorded as an ace medicine for cleansing protecting liver in Chinese Pharmacopoeia has been main constitutions many herbal tea formulas China centuries. also a traditional folk Europe other countries Asia. Pentacyclic triterpenoids are major class bioactive compounds produced P. . However, their biosynthetic mechanism remains to be elucidated. Here, we report chromosome‐level reference genome using...
MicroRNAs (miRNAs) are a group of endogenous small non-coding RNAs in plants. They play critical functions various biological processes during plant growth and development. Salvia miltiorrhiza is well-known traditional Chinese medicinal with significant medicinal, economic, academic values. In order to elucidate the role miRNAs S. miltiorrhiza, six RNA libraries from mature roots, young stems, leaves, leaves flowers one degradome library mixed tissues were constructed. A total 184 miRNA...
Abstract Aristolochic acids (AAs) and their derivatives are present in multiple Aristolochiaceae species that have been or being used as medicinal materials. During the past decades, AAs received increasing attention because of nephrotoxicity carcinogenicity. Elimination from materials using biotechnological approaches is important for improving medication safety. However, it has not achieved limited information available on AA biosynthesis. Here, we report a high-quality, reference-grade...
Salvia miltiorrhiza is a model medicinal plant with significant economic and value. Its roots produce group of diterpenoid lipophilic bioactive components, termed tanshinones. Biosynthesis regulation tanshinones has attracted widespread interest. However, the methylome S. not been analysed regulatory mechanism DNA methylation in tanshinone production largely unknown. Here we report single-base resolution methylomes from leaves. Comparative analysis revealed differential patterns for CG, CHG,...
Prunella vulgaris is an important material for Chinese medicines with rosmarinic acid (RA) as its index component. Based on the chromosome-level genome assembly we obtained recently, 51 RA biosynthesis-related genes were identified. Sequence feature, gene expression pattern and phylogenetic relationship analyses showed that 17 of them could be involved in biosynthesis. In vitro enzymatic assay PvRAS3 catalyzed condensation p -coumaroyl-CoA caffeoyl-CoA pHPL DHPL. Its affinity toward was...
Tumor cells trigger angiogenesis through overexpression of various angiogenic factors including vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang1). Therefore, inhibition the expression both VEGF Ang1, initial step tumor angiogenesis, is a promising strategy for cancer chemoprevention therapy. Grape seed proanthocyanidins (GSPs) are widely consumed dietary supplements that have antitumor activity. Due to their polymeric structure, GSPs poorly absorbed along gastrointestinal...
Abstract Salvia miltiorrhiza is a well-known material of traditional Chinese medicine. Understanding the regulatory mechanisms phenolic acid biosynthesis and metabolism are important for S. quality improvement. We report here that contains 19 polyphenol oxidases ( PPOs ), forming largest PPO gene family in plant species to our knowledge. Analysis structures sequence features revealed conservation divergence SmPPOs. SmPPOs were differentially expressed tissues eight them predominantly phloem...
Abstract Neoadjuvant PD-1 inhibitor is promising in cutaneous melanoma but remains unknown acral (AM). This phase Ib trial study (Clinicaltrials.gov NCT04197882) assessed the efficacy and safety of combination neoadjuvant oncolytic virus orienX010 (ori) anti-PD-1 toripalimab (tori) for resectable AM. Thirty patients stage III/IV received therapy ori tori 12 weeks before surgery, followed by adjuvant treatment with 1 year. Primary endpoints were radiographic pathological response rates,...
Gibberellin (GA), a classical phytohormone, plays significant roles in plant growth and development. It shares the important intermediate diphosphate precursor, GGPP, with main lipophilic bioactive components, diterpenoid tanshinones Salvia miltiorrhiza Bunge, one of most Traditional Chinese Medicine materials an emerging model medicinal plant. Analysis GA metabolism regulation may help to demonstrate biological functions GAs crosstalk between tanshinone biosynthesis S. miltiorrhiza....
Abstract Immune checkpoint inhibitors (ICIs) have provided new hope for melanoma patients, however, not all patients benefit. Furthermore, ICI-related therapies cause significant immune-related adverse events that adversely affect patient outcomes. Therefore, there is a pressing need reliable biomarkers to identify most likely benefit from these treatments. In this study, we employed an extracellular vesicles (EVs) protein expression array explore the longitudinal membrane profiles of...