A5072302646- Cancer Genomics and Diagnostics
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Hepatitis C virus research
- Molecular Biology Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Renal cell carcinoma treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- Virus-based gene therapy research
- Poxvirus research and outbreaks
- Toxin Mechanisms and Immunotoxins
- Prenatal Screening and Diagnostics
- Cancer-related gene regulation
- Cytomegalovirus and herpesvirus research
- Bladder and Urothelial Cancer Treatments
- Immunotherapy and Immune Responses
- Renal and related cancers
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Acne and Rosacea Treatments and Effects
- interferon and immune responses
Baruch S. Blumberg Institute
2016-2025
JBS Science (United States)
2025
Drexel University
2009-2024
Doylestown Hospital
2011-2015
Philadelphia University
2014
University of Michigan
2006
St. Michael's Hospital
2006
Dana-Farber Cancer Institute
2006
Thomas Jefferson University
1998-2004
Louisiana State University Health Sciences Center New Orleans
1996-1997
Our previous studies demonstrated that urine contains DNA derived from the circulation and this originated, in part, organ sites tumors distal to urinary tract. To explore potential use of as compared other body fluids a source for circulating cancer detection, concentration frequency detection mutated Kristin‐ ras (K ‐ras ) serum, plasma, were examined. The was similar but plasma significantly lower than either or serum ( P < 0.05). When 10 μL fluid used each mutation assay, K comparable...
Herpes simplex virus type 1 (HSV-1) infection on the murine cornea induces an intense inflammatory response which can lead to blindness. This disease, known as herpes stromal keratitis, be prevented by timely passive transfer of monoclonal antibody specific for viral glycoprotein D (gD). Precisely how treatment prevents excessive corneal inflammation is not known. In this study we investigated whether chemokine mRNA expression inhibited treatment. Total cellular RNAs isolated from normal...
Hepatoma Research is an open access journal and focuses on all topics related to hepatoma. The following articles are especially welcome: pathogenesis, clinical examination early diagnosis of hepatoma, complications their preventions treatments, etc.
Hepatitis B virus (HBV) is a hepatotropic causing hepatitis, cirrhosis and hepatocellular carcinoma (HCC). The methylation status of the HBV DNA in its different forms can potentially provide insight into pathogenesis HBV-related liver diseases, including HCC, however this unclear. goal study to obtain comprehensive profiles three putative CpG islands infected livers, with respect disease progression. extent these was first assessed using bisulfite PCR sequencing small set tissue samples,...
We demonstrated previously that urine contains low-molecular-weight (LMW) (<300 bp), circulation-derived DNA can be used to detect cancer-specific mutations if a tumor is present. The goal of this study was develop an assay the colorectal cancer (CRC)–associated, circulation-derived, epigenetic marker hypermethylated vimentin gene (mVIM) in patients with CRC. An artificial 18-nucleotide sequence tagged at 5′ end primers first PCR cycle increase amplicon size, which then integrated into...
The latency-associated transcripts (LATs) of herpes simplex virus type 1 (HSV-1) are the only viral gene products that accumulate to abundant levels in latently infected cells. Others have reported species 2.0, 1.50, and 1.45 kb; 2.0-kb is seen productively cells, there evidence it behaves as an intron. We examined LATs both trigeminal ganglia mice cultures monkey CV-1 After glyoxalation, RNA was subjected high-resolution agarose gel electrophoresis Northern (RNA) analysis, a procedure...
A bstract : small portion of DNA from apoptotic cells escapes complete degradation, appears in blood as oligonucleosomal‐size fragments, is excreted the urine, and can be used for diagnostic purposes. More detailed study revealed that transrenal (Tr‐DNA) belongs to a relatively low molecular‐weight (150–250 bp) fraction, thereby requiring more careful attention methods employed purification analysis. For example, here it demonstrated QIAamp kit purifies primarily high serum, whereas...
BALB/c mice infected on the scarified cornea with herpes simplex virus type 1 strain 35 [HSV-1(35)] rarely developed ocular disease even at challenge doses as high 10(7) PFU per eye. In contrast, HSV-1(RE) consistently induced stromal keratitis an inoculum of 2 x 10(4) PFU. The goal this study was to determine reason for difference in virulence between two HSV strains. Both HSV-1 strains replicated similar titers excised corneal "buttons." However, after vivo infection cornea, growth evident...
We have previously demonstrated that mutated DNA derived from the circulation can be detected in urine and predominantly exists as fragments <1 kb. To preferentially isolate trans‐renal urine, we developed a method using carboxylated beads to separate high‐MW (1 kb or larger) low‐MW urine. A primer set for 18s rRNA (generating PCR product of 872 bp) was designed optimized real‐time quantification templates. evaluate method, samples 5 volunteers with no known diseases 36 patients various...
Aberrant methylation of the promoter, P2, and first exon, E1, regions tumor suppressor gene RASSF1A, have been associated with hepatocellular carcinoma (HCC), albeit poor specificity. This study analyzed profiles P1, P2 E1 to identify region which most specifically corresponds HCC evaluate potential this methylated as a biomarker in urine for screening.Bisulfite DNA sequencing quantitative methylation-specific polymerase chain reaction assays were performed compare 56 CpG sites isolated from...
Abstract Background and Aims Hepatitis B virus (HBV) generates a double-stranded linear DNA (dslDNA) byproduct during replication. This dslDNA can undergo intermolecular intramolecular nonhomologous end-joining (NHEJ) recombination, resulting in viral integration derived covalently closed circular DNAs (dsl-cccDNAs), respectively. The insertions deletions (INDELs) at the site around direct repeat (DR) 1 motif have been used to differentiate dsl-cccDNA from authentic cccDNA. frequency...
ABSTRACT Background HBsAg can be derived from intrahepatic cccDNA and integrated HBV DNA (iDNA). We examined the iDNA liver tissues of 24 HBeAg(+) 32 HBeAg(−) treatment-naive CHB participants. Methods Liver were obtained North American Hepatitis B Research Network (HBRN). For analysis, was heat-denatured digested by plasmid-safe ATP-dependent DNase to remove rcDNA prior qPCR. detection, total subjected hybridization-targeted next generation sequencing (HBV-NGS) assay. The HBV-host junction...
This study characterized a hepatitis B virus (HBV) hybridization-capture next-generation sequencing (HBV-NGS) assay and applied it to develop model for estimating the integrated HBV DNA (iDNA) quantity genetics liquid biopsy. Using monomers reconstituted cell line (SNU398, Hep3B, PLC/PRF/5), HBV-NGS demonstrated high coverage uniformity, reproducibility across genotypes A-D, 0.1% sensitivity detecting iDNA. The iDNA sequence structures from SNU398 Hep3B are reported. An estimation was...
quantified, but they could potentially be particularly useful in developing countries.
Hepatocellular carcinoma is one of the fastest growing cancers in US and has a low survival rate, partly due to difficulties early detection. Because HCC's high heterogeneity, it been suggested that multiple biomarkers would be needed develop sensitive HCC screening test. This study applied random forest (RF), machine learning technique, proposed two novel models, fixed sequential (FS) two-step (TS), for comparison with commonly used statistical techniques, logistic regression (LR)...
A rabbit ocular model of epinephrine-induced herpes simplex virus type 1 reactivation was employed to study the effect a deletion in latency-associated transcript domain. viral construct derived from 17Syn+, designated 17deltaSty, has 370 nucleotides between genomic positions 118880 and 119250. 17deltaSty been shown reactivate with wild-type kinetics explants trigeminal ganglia latently infected mice. To determine behavior this mutant an vivo, inducible system, corneas were or its rescuant,...