A5030293525- Pluripotent Stem Cells Research
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Biomedical Ethics and Regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Renal and related cancers
- Mesenchymal stem cell research
- Insect Resistance and Genetics
- Pancreatic function and diabetes
- CAR-T cell therapy research
- Congenital heart defects research
- Science, Research, and Medicine
- Transgenic Plants and Applications
- Biomedical and Engineering Education
- Protein purification and stability
- Electrospun Nanofibers in Biomedical Applications
- Insect and Arachnid Ecology and Behavior
- Ethics in Clinical Research
- Epigenetics and DNA Methylation
- Energy Harvesting in Wireless Networks
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Medical Imaging and Pathology Studies
- Virus-based gene therapy research
- Insect Utilization and Effects
University at Buffalo, State University of New York
2020-2025
Boston University
2012-2024
Boston Medical Center
2012-2024
International Society for Cellular Therapy
2017
Pulmonary Associates
2011
National Institute of Diabetes and Digestive and Kidney Diseases
2008-2010
National Institutes of Health
2007-2008
UCLouvain
2001-2008
ABSTRACT Candida albicans is a commensal colonizer of the gastrointestinal tract humans, where it coexists with highly diverse bacterial communities. It not clear whether this interaction limits or promotes potential C. to become an opportunistic pathogen. Here we investigate between and three species streptococci from viridans group, which are ubiquitous abundant oral bacteria. The ability form biofilms Streptococcus oralis , sanguinis gordonii was investigated using flow cell devices that...
Cell and gene therapies (CGTs) are progressively entering into clinical practice in different parts of the world. The International Society for & Gene Therapy (ISCT), a global scientific society, has been committed since 1992 to supporting developing knowledge on applications CGTs. Considering number products that have approved and, some cases, withdrawn recent years, ISCT would like present brief annual report CGTs with marketing authorization (MA) regions. This article reflects dynamic...
The in vitro directed differentiation of pluripotent stem cells (PSCs) through stimulation developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. However, there has been significant uncertainty regarding how to separately derive lung vs. thyroid epithelial lineages, since these two each originate from Nkx2-1+ foregut progenitors, and the minimal claimed regulate their distinct lineage specification vivo have varied...
Islet transplantation offers improved glucose homeostasis in diabetic patients, but of islets is limited by the supply donor pancreases. Undifferentiated precursors hold promise for cell therapy because they can expand before differentiation to produce a large functional insulin-producing cells. Previously, we described proliferative populations human islet-derived precursor cells (hIPCs) from adult islets. To show potential hIPCs, which do not express insulin mRNA after at least 1,000-fold...
Development of an anti-SARS-CoV-2 therapeutic is hindered by the lack physiologically relevant model systems that can recapitulate host-viral interactions in human cell types, specifically epithelium lung. Here, we compare induced pluripotent stem (iPSC)-derived alveolar and airway epithelial cells to primary lung controls, focusing on expression levels genes for COVID-19 disease modeling. iPSC-derived type II-like (iAT2s) lineages express key transcripts associated with identity majority...
Multipotent Nkx2-1-positive lung epithelial primordial progenitors of the foregut endoderm are thought to be developmental precursors all adult lineages. However, little is known about global transcriptomic programs or gene networks that regulate these gateway in vivo. Here we use bulk RNA-sequencing describe unique genetic program vivo murine and computationally identify signaling pathways, such as Wnt Tgf-β superfamily involved their cell-fate determination from pre-specified embryonic...
Abstract While the generation of many lineages from pluripotent stem cells has resulted in basic discoveries and clinical trials, derivation tissue-specific mesenchyme via directed differentiation markedly lagged. The lung-specific is particularly important since this tissue plays crucial roles lung development disease. Here we generate a mouse induced cell (iPSC) line carrying mesenchymal reporter/lineage tracer. We identify pathways (RA Shh) necessary to specify find that iPSC-derived...
The clinical importance of anterior foregut endoderm (AFE) derivatives, such as thyrocytes, has led to intense research efforts for their derivation through directed differentiation pluripotent stem cells (PSCs). Here, we identify transient overexpression the transcription factor (TF) NKX2-1 a powerful inductive signal robust thyrocyte-like from mouse PSC-derived AFE. This effect is highly developmental stage specific and dependent on FOXA2 expression levels precise modulation BMP FGF...
The field of regenerative medicine, including cellular immunotherapies, is on a remarkable growth trajectory. Dozens cell-, tissue- and gene-based products have received marketing authorization worldwide while hundreds-to-thousands are either in preclinical development or under clinical investigation phased trials. However, the promise therapies has also given rise to global industry direct-to-consumer offerings prematurely commercialized cell cell-based with unknown safety efficacy...
This position paper reviews the Expanded Access pathway for cell and gene therapies, examining its critical role at nexus of patient need, regulatory frameworks, scientific advancement. Spearheaded by International Society Cell & Gene Therapy's Working Group, it explores how investigational therapies are accessed outside clinical trials patients with serious or life-threatening conditions when no approved alternatives exist. to therapy products specific interest because many times bespoke,...
Retinoschisin (RS1) is a retina-specific secreted protein encoding conserved discoidin domain sequence. As an adhesion molecule, RS1 preserves the retinal cell architecture and promotes visual signal transduction. In young males, loss-of-function mutations in X-linked retinoschisis gene cause retinoschisis, form of progressive blindness. Neither structure nor nature its anchoring organization on plasma membranes fully understood. The C2 domains coagulation factors V VIII are known to...
Abstract Alveolar type 2 epithelial cells (AT2s) derived from human induced pluripotent stem (iAT2s) have rapidly contributed to our understanding of AT2 function and disease. However, while iAT2s are primarily cultured in three-dimensional (3D) Matrigel, a matrix cancerous mouse tissue, it is unclear how physiologically relevant will impact phenotype. As extracellular (ECM) recognized as vital component directing cellular differentiation, we sought derive hydrogels decellularized lung...