A5038725773- Blood properties and coagulation
- Hormonal Regulation and Hypertension
- Blood Coagulation and Thrombosis Mechanisms
- Retinal Development and Disorders
- Platelet Disorders and Treatments
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Protease and Inhibitor Mechanisms
- Ion Transport and Channel Regulation
- Estrogen and related hormone effects
- Cancer Cells and Metastasis
- Angiogenesis and VEGF in Cancer
- Corneal Surgery and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Venous Thromboembolism Diagnosis and Management
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Surface Modification and Superhydrophobicity
- Intraperitoneal and Appendiceal Malignancies
- Retinal Diseases and Treatments
- Ovarian cancer diagnosis and treatment
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
Université Paris Cité
2014-2024
Inserm
2014-2024
Hôpital Lariboisière
1990-2024
ParisTech
2019-2024
Instituto de Investigación Sanitaria La Fe
2023
Academy of Sciences of the Republic of Tajikistan
2017-2022
Hôtel-Dieu de Paris
1986-2019
Sorbonne Paris Cité
2016-2019
Délégation Paris 7
2014-2019
Inserm Transfert
2017-2019
Our vision of cancer has changed during the past decades. Indeed tumors are now perceived as complex entities where tumoral and stromal components interact closely. Among different elements tumor stroma cellular component play a primordial role. Bone Marrow derived mesenchymal cells (MSCs) attracted to sites support growth. Endothelial (ECs) major role in angiogenesis. While literature documents many aspects cross talk between cells, direct hetero-cellular contact is not clearly established....
Background— The mechanisms of thrombosis on plaque erosion are poorly understood. We examined the potential role endothelial apoptosis in and vessel thrombosis. Methods Results— Segments New Zealand White rabbit femoral arteries were temporarily isolated vivo. One artery was incubated with staurosporin for 30 minutes, whereas contralateral saline served as control. Three days later, evaluated angiographically histologically. TUNEL score layer significantly increased staurosporin-treated...
Albumin nanoparticles (NP) were proved to be effective and safe carriers for delivering anticytomegaloviral compounds in the vitreous. NP improved antiviral activity of both ganciclovir phosphodiester oligonucleotide analog formivirsen. appeared fusogenic able target nucleus cells. In addition, these drug well tolerated when administered by intravitreal route did not induce autoimmune reactions. Keywords: albumin, nanoparticles, ganciclovir, fomivirsen, oligonucleotides, delivery, retinitis
The leukemia‐associated cell surface antigen p 24 is found on normal platelets as well Bernard Soulier syndrome and thrombasthenia type I platelets. ALB 6 IgG (a monoclonal antibody against 24) induces the aggregation of from donors but not thrombasthenia. In contrast, Fab inhibits platelet induced by collagen, ADP, thrombin, ionophore A 23187 IgG. results suggest that interferes with a mechanism common to all pathways; possible mechanisms are discussed.
Hyperthermic intraperitoneal chemotherapy (HIPEC) has shown promise in treatment of ovarian carcinosis. Despite its efficiency for the peritoneal carcinosis from digestive tract neoplasia, it failed to demonstrate significant benefit cancers. It is therefore essential understand mechanism underlying resistance HIPEC Mesenchymal stem cells (MSC) play an important role development cancer metastasis and treatments. A recent study suggests that MSCs may be cytotoxic upon heat shock. In contrast,...
The microenvironment plays a major role in the onset and progression of metastasis. Epithelial ovarian cancer (EOC) tends to metastasize peritoneal cavity where interactions within might lead chemoresistance. Mesothelial cells are important actors homeostasis; we determined their acquisition chemoresistance tumours.We isolated an original type stromal cells, referred as "Hospicells" from ascitis patients with carcinosis using limiting dilution. We studied ability confer through...
The early peritoneal invasion of epithelial ovarian cancer (EOC) by tumoral aggregates presents in ascites is a major concern. role the microenvironment seems to be important this process but lack adequate models study cellular interactions between cells and stromal does not allow uncover molecular pathways involved. Our goal was (OCC) mesenchymal stem (MSC) using 3D model.We used millimetric pieces amniochorionic membrane - referred as amniotic scaffold (AMS) create nodules mimicking EOC...
Minimal residual disease is the main issue of advanced ovarian cancer treatment. According to literature and previous results, we hypothesized that Mesenchymal Stromal Cells (MSC) could support this minimal by protecting cells (OCC) from chemotherapy. In vitro study confirmed MSC induce OCC chemoresistance without contact using transwell setting. Further experiments showed induced was dependent on IL-6 stimulation. We combined meticulous in profiling tumor xenograft models role MSC/OCC...
Abstract Localization of plasmin activity on leukocyte surfaces plays a critical role in fibrinolysis as well pathological and physiological processes which cells must degrade the extracellular matrix order to migrate. The binding plasminogen leukocytic cell lines induces 30‐ 80‐fold increase rate activation by tissue‐type (tPA) urokinase‐type (uPA) activators. In present study we have examined α‐enolase surface. We produced characterized monoclonal antibody (MAb) 11G1 against purified...
The S‐antigen is a protein of photoreceptors, mainly known for its autoantigenic properties in mammals, which widely distributed the retina vertebrates and photoreceptor organs invertebrates. Using three monoclonal antibodies specific different epitopes S‐antigen, this study complements our previous data on retinal rods cones presents new results photosensory cells pineal complex. Immunoreactivity was found (i) cones, (ii) cone‐like modified cells, pinealocytes organ vertebrates, (iii)...
The microenvironment is known to play a dominant role in cancer progression. Cells closely associated with tumoral cells, named hospicells, have been recently isolated from the ascites of ovarian patients. Whilst these cells present no specific markers cell lineages, they do share some homology bone marrow-derived or adipose tissue-derived human mesenchymal stem (CD9, CD10, CD29, CD146, CD166, HLA-1). We studied hospicells carcinoma In vitro, had effect on growth lines OVCAR-3, SKOV-1 and...
The orange-peel derived terpene d-Limonene, probably through its metabolite, perillyl alcohol (POH), has been reported to have tissue-repair properties. Two murine models of respectively 12-O-Tetradecanoylphorbol-13-Acetate (TPA)-induced dermatitis and mechanical skin lesion were used here assess the efficacy d-Limonene or POH applied topically. Macroscopic microscopic evaluation lesions was performed as well that P-selectin expression, together with measurements serum concentrations IL-1β,...
Purpose To determine the in vivo effects of several particulate steroids on microvascular perfusion by using intravital microscopy a mice model and to investigate vitro interactions between these red blood cells (RBCs). Materials Methods The study was conducted agreement with guidelines National Committee Ethic Reflection Animal Experimentation. By mouse cremaster muscle, were assessed. Four five allocated each following treatment groups: saline solution, dexamethasone sodium phosphate,...
Catalyst-free ring-opening polymerization (ROP) strategy was developed to overcome the disadvantage of incomplete and expensive removal catalyst used during multistep wet chemical processes. Nano-sized biocompatible low molecular weight poly(ε-carolactone)-poly(ethylene glycol) (PCL-PEG) copolymer coatings were deposited via a single-step, low-pressure, pulsed-plasma process. Experiments performed at different monomer feed ratio effective plasma power. The analyzed by XPS, as well MALDI ToF....
Tumor microenvironement is an important actor of ovarian cancer progression but the relations between mesenchymal cells and remain unclear. The objective this study was to determine cells' biological modifications induced by cells. To address issue, we used two different cell lines (NIH:OVCAR3 SKOV3) co-cultured them with Upon co-culture populations were sorted their transcriptome properties. Transcriptomic analysis revealed three biological-function gene clusters enriched upon contact These...