A total of 216 weaning pigs were used in 2 experiments to determine the effects dietary supplementation yeast culture (YC) at different dose levels on growth performance, nutrient digestibility, intestinal morphology, microflora, and immune response weanling whether YC can be a candidate replace antibiotic promoters (AGP). In Exp. 1, 192 (7.5 +/- 0.2 kg BW) weaned 28 d age randomly allotted 6 treatments: 1) control (without AGP or YC); 2) (chlortetracycline, 80 mg/kg); 3) 2.5 g/kg (Diamond V...

Leucine and isoleucine possess antioxidative anti-inflammatory properties. However, their underlying protective mechanisms against oxidative damage remain unknown. Therefore, in this study, the mechanism of leucine H2O2-induced a bovine mammary epithelial cell lines (MAC-T cells) were investigated. Briefly, MAC-T cells exposed or free to H2O2 incubated with different combinations isoleucine. The cellular relative proliferation rate viability, stress indicators, inflammatory factors...

Abstract Background Silage is achieved by anaerobic fermentation of lactic acid bacteria. However, harvest stage and Lactiplantibacillus plantarum ( L. ) content affect the changes microbial community in silage, which play an important role silage quality mycotoxin levels. The current study evaluated effects exerted application on fermentation, mycotoxins bacterial whole-crop corn at different stages. fresh whole plant from same area was harvested during milk dough stage, following each...

This study was aimed at determining the effective ingredients of yeast culture (YC) for animal breeding. First, contents YCs obtained from various fermentation times were detected using gas-chromatography. A total 85 compounds identified. Next, 336 Arbor Acres (AA) broilers randomly divided into seven experimental groups and fed a basal diet, diets supplemented with times, or SZ1 (a commercial YC product). significant increase in body weight gain (BWG) decrease feed conversion ratio (FCR) AA...

High levels of starch is known to have positive effects on both energy supply and milk yield but increases the risk rumen acidosis. The use sugar as a non-structural carbohydrate could circumvent this while maintaining benefits, its that simultaneous are not well-understood. This study aimed evaluate different combinations concentrations ruminal fermentation bacterial community composition in vitro 4 ×4 factorial experiment. Sixteen dietary treatments were formulated with (6, 8, 10, 12% dry...

Abstract Background In highly intensive dairy farms, cows often suffer from metabolic disorders that cause severe oxidative stress. Objectives This study aimed to observe correlations and associations of stress‐related indicators with milk compositions metabolites. Methods Twenty‐two multiparous Holstein in early lactation were randomly selected a commercial farm. The morning was collected for composition metabolites analysis. Blood sampled via the tail vein analyze (reactive oxygen species,...

Hemicellulose is an important polysaccharide in ruminant nutrition, but it has not been studied as thoroughly cellulose. Further research needed to explore supplements that can improve its digestibility and ruminal buffering effects. Our previous demonstrated the efficacy of oxalic acid (OA) essential nutrient yeast culture (YC) for improving rumen fermentation performance. Consequently, we conducted vitro digestion experiments examine effects YC OA on bacterial composition. Two diets...

ABSTRACT The rumen microbiota plays a vital role in the nutrient metabolism affecting growth of velvet antler. However, fermentation patterns and dynamics across stages antler remain largely unexplored. Here, we employed an vitro approach to assess parameters microbial composition liquid sika deer during early (EG), metaphase (MG), fast (FG) phases . Our findings indicated that levels short-chain fatty acids (SCFAs), ammonia nitrogen, gas production increased over time all three groups....

Determining hemicellulose (HM) degradation is crucial for evaluating the nutritional value of ruminant diets. Our previous study showed that oxalic acid (OA) regulates rumen fermentation. Building on this research, present examined effects OA supplementation in different diets sheep fermentation, microbial diversity, and metabolite production vitro. Diets with low high HM levels (10.3% 17%, respectively) supplemented seven concentrations (0, 2.5, 5, 10, 20, 40, 80 mg/kg DM) were evaluated....

This study evaluated the effects of antimicrobial peptide (AMP) and tributyrin (TB) on dairy calves in terms growth performance, immunity, oxidative stress, intestinal microflora. A total 40 female were divided into four treatment groups (

Deoxynivalenol contamination in feed and food, pervasive from growth, storage, processing, poses a significant risk to dairy cows, particularly when exposed high-starch diet; however, whether diet exacerbates these negative effects remains unclear. Therefore, we investigated the combined impact of deoxynivalenol dietary starch on production performance, rumen function, health cows using metabolomics 16 S rRNA sequencing. Our findings suggested that both high- low-starch diets contaminated...

This study was conducted to evaluate the effects of yeast culture (YC) supplementation on growth performance, apparent nutrient digestibility and caecal microflora broiler chickens. A total 360 one-day-old Arbor Acres chickens were randomly assigned six dietary treatments containing 0.2%, 0.4%, 0.6%, 0.8% 1% YC. The experiment lasted for 42 days. Diet faecal samples collected analysis dry matter, crude protein, ether extract, calcium phosphorus. Caecal microbiota days 21 measured using...

Abstract The effects of yeast culture (YC) supplementation and the dietary ratio non‐structural carbohydrate to fat (NSCFR) on growth performance, carcass traits fatty acid profile longissimus dorsi (LD) muscle in lambs were determined a 2 × 3 full factorial experiment. Thirty‐six Small‐tailed Han randomly divided into six groups with replicates per group. fed one pelleted total mixed rations (TMRs) for 60 days after 15 adaption days. formed by two NSCFRs (11.37 4.57) three YC levels (0, 0.8...

Even if breed, parity, dietary and environmental management are same, dairy cows still have notable differences in milk yield that may be underpinned by physiologic differences.This study aimed to investigate the physiological dissimilarities of with different yields.Thirty were sorted into high milk-yielding (group H: 58.93±2.31 kg/day), moderate M: 44.99±0.54 low L: 24.99±6.83 kg/day) according yield. Blood was collected serum parameters assessed. Rumen fluid for evaluation rumen...

Processing can affect milk properties and alter the composition of metabolites, which has corresponding effects on flavor quality. It is quite important to study safe quality control processing. Therefore, purpose this was identify metabolites at different steps ultra-high-temperature-sterilized (UHT) processing using gas chromatography-mass spectrometry (GC-MS). These included raw milk, pasteurized (80°C for 15 s), semi-finished (after pasteurizing, it homogenized 75°C with pressure 250...

Inositol is a bioactive factor that widely found in nature; however, there are few studies on its use ruminant nutrition. This study investigated the effects of different inositol doses and fermentation times rumen microbial diversity, as well levels blood metabolites sheep. Rumen parameters, after were determined vitro . According to results, six small-tailed Han sheep fitted with permanent fistulas used 3 × Latin square feeding experiment where was injected into twice day fluid samples...

Introduction Peripartal cows are susceptible to a negative energy balance due inadequate nutrient intake and high requirements for lactation. Improving the metabolism of perinatal dairy is crucial in increasing production cows. Methods In this study, we investigated impact rumen-protected branched-chain amino acid (RPBCAA) on performance, lipid metabolism, oxidative stress, immune function primiparous using metabolomics through single-factor experiment. Twenty healthy Holstein were selected...

Yeast culture (YC) enhances ruminant performance, but its functional mechanism remains unclear because of the complex composition YC and uncertain substances affecting rumen fermentation. The objective this study was to determine effective metabolites in by exploring effects on fermentation vitro, growth slaughter serum index, parameters, microorganisms, lambs. In Trial 1, various YCs were successfully produced, providing raw materials for identifying metabolites. experiment divided into 5...

Abstract The objective of this study was to investigate the effects yeast culture (YC) on growth performance, caecal microbial community and metabolic profile broilers. A total 350 1‐day‐old healthy Arbor Acres broilers were randomly assigned seven treatment groups. first group received a basal diet without YC supplementation, whereas remaining groups supplemented with either fermented for 12, 24, 36, 48 or 60 hr, commercial product (SZ2). MiSeq 16S rRNA high‐throughput sequencing used...

Cis-9-conjugated, trans-11-conjugated linoleic acid (CLA) is known for its positive activities on human health. The synthesis of cis-9, trans-11 CLA in mammary glands generally thought to be catalyzed by stearoyl-CoA desaturase 1 (SCD1), but this has not been rigorously established. In study, we hypothesized that the inhibition SCD1 (by CAY10566) would block bovine alveolar cells (MAC-T) cells. Results showed MAC-T incubated with 10 nM CAY10566 12 h (CAY) produced less (p < 0.01), lower...

The objective of the study was to determine effects antibiotics, yeast culture (YC), and Lactobacillus (LC) on gut microbiome metabolites in serum caecum weaning piglets. Twenty-four piglets were divided into four treatment groups: control, antibiotic (1% chlortetracycline), 1.8% 1.6% groups (LC). Each group had six replicated pens with one pig per pen. Feed water available ad libitum. Dietary supplementation YC LC increased abundance phylum, Firmicutes, decreased Proteobacteria. Beneficial...

The objective of the study was to elucidate stearoyl-coenzyme A desaturase (SCD1)-dependent gene network c9, t11-CLA biosynthesis in MAC-T cells from an energy metabolism perspective. were divided into CAY group (firstly incubated with CAY10566, a chemical inhibitor SCD1, then trans-11-octadecenoic acid, (TVA)), TVA (only TVA), and control (without CAY, TVA). t11-CLA, contents determined by gas chromatography. mRNA levels SCD1 candidate genes analyzed via real-time PCR. Tandem mass tag...