A5101404845- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Respiratory Support and Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Thermal Regulation in Medicine
- Inflammation biomarkers and pathways
- Cardiac Arrest and Resuscitation
- Pulmonary Hypertension Research and Treatments
- Thermoregulation and physiological responses
- Additive Manufacturing and 3D Printing Technologies
- Spinal Cord Injury Research
- Liver Disease and Transplantation
- 3D Printing in Biomedical Research
- Asthma and respiratory diseases
- Viral gastroenteritis research and epidemiology
- Nutrition and Health in Aging
- Spinal Dysraphism and Malformations
- Optical Imaging and Spectroscopy Techniques
- Neonatal Respiratory Health Research
- Insect symbiosis and bacterial influences
- Respiratory viral infections research
- Angiogenesis and VEGF in Cancer
Tibet Autonomous Region People's Hospital
2018-2024
University of Maryland, Baltimore
2015-2022
Arizona State University
2011-2021
A 3D printing methodology for the design, optimization, and fabrication of a custom nerve repair technology regeneration complex peripheral injuries containing bifurcating sensory motor pathways is introduced. The scaffolds are deterministically fabricated via microextrusion principle using models, which reverse engineered from patient anatomies by scanning. augmented with printed biomimetic physical cues (microgrooves) path‐specific biochemical (spatially controlled multicomponent...
Autophagy is a catabolic process that degrades cytoplasmic constituents and organelles in the lysosome, thus serving an important role cellular homeostasis protection against insults.We previously reported defects autophagy contribute to neuronal cell damage traumatic spinal cord injury (SCI).Recent data from other inflammatory models implicate regulation of immune responses, with low levels autophagic flux associated pro-inflammatory phenotypes.In present study, we examined effects...
Previously, our group engineered a plant-derived monoclonal antibody (MAb pE16) that efficiently treated West Nile virus (WNV) infection in mice. In this study, we developed pE16 variant consisting of single-chain variable fragment (scFv) fused to the heavy chain constant domains (CH) human IgG (pE16scFv-CH). and pE16scFv-CH were expressed assembled Nicotiana benthamiana ∆XF plants, glycosylation mutant lacking plant-specific N-glycan residues. Glycan analysis revealed (∆XFpE16scFv-CH)...
Previously, our group engineered a plant-derived monoclonal antibody (MAb) (pHu-E16) that efficiently treated West Nile virus (WNV) infection in mice. In this study, we developed several pHu-E16 variants to improve its efficacy. These included single-chain variable fragment (scFv) of fused the heavy chain (HC) constant domains (CH(1-3)) human IgG (pHu-E16scFv-CH(1-3)) and tetravalent molecule (Tetra pHu-E16) assembled from pHu-E16scFv-CH(1-3) with second pHu-E16scFv light (LC) region. Tetra...
The threat of West Nile virus (WNV) epidemics necessitates the development a technology platform that can produce reagents to support detection and diagnosis rapidly inexpensively. A plant expression system is attractive for protein production due its low-cost high-scalability nature ability make appropriate posttranslational modifications. Here, we investigated feasibility using plants two WNV diagnostic address current cost scalability issues. We demonstrated DIII antigen E16 monoclonal...
Introduction: C-X-C motif chemokine 5 is primarily chemotactic for neutrophils and previously shown to increase in the bronchoalveolar lavage fluid of patients with chronic obstructive pulmonary disease. However, whether levels correlate lung function decline or mouse model disease was not clear. Methods: The induced by cigarette smoke exposure. Plasma/serum were obtained from disease; assessed correlated functions granulocyte-colony stimulating factor levels, respectively. Results:...
We described the rapid production of domain III (DIII) envelope (E) protein in plants as a vaccine candidate for West Nile Virus (WNV). Using various combinations vector modules deconstructed viral expression system, DIII was produced three subcellular compartments leaves Nicotiana benthamiana by transient expression. expressed at much higher levels when targeted to endoplasmic reticulum (ER) than that chloroplast or cytosol, with accumulation level up 73 μ g per gram leaf fresh weight...
In this study, we developed a hepatitis B core antigen (HBcAg)-based virus-like particle (VLP) that displays the West Nile virus (WNV) Envelope protein domain III (wDIII) as vaccine candidate for WNV. The HBcAg-wDIII fusion was quickly produced in Nicotiana benthamiana plants and reached high expression level of approximately 1.2 mg per gram leaf fresh weight within six days post gene infiltration. Electron microscopy gradient centrifugation analysis indicated introduction wDIII did not...
Brain injury is the main cause of mortality and morbidity after cardiac arrest (CA). Changes in cerebral blood flow (CBF) reperfusion are associated with brain recovery. To characterize relative CBF (rCBF) CA, 14 rats underwent 7 min asphyxia-CA were randomly treated 6 h post-resuscitation normothermic (36.5–37.5℃) or hypothermic- (32–34℃) targeted temperature management (TTM) (N = 7). rCBF was monitored by a laser speckle contrast imaging (LSCI) technique. recovery evaluated neurologic...
Backgrounds and Aims: It is well known that angiogenesis contributes to the progression of chronic obstructive pulmonary disease (COPD) by initiating remodeling bronchial vasculature. However, specific molecular mechanisms are incompletely understood. This research aimed explore whether endostatin, a member endogenous antiangiogenic proteins, biomarker in COPD plays role COPD. Methods: 100 stable patients, 130 patients with acute exacerbation (AECOPD) 68 healthy volunteers were recruited...
Cardiac arrest (CA) results in global brain ischemia. To explore the role of cerebral blood flow (CBF) during ischemia, laser speckle contrast imaging (LSCI), a full-field high-resolution optical technique, was used for real-time monitoring fluctuations CBF rat model asphyxial-CA. The temporal changes were characterized and relationship between mean arterial pressure (MAP) evaluated. Asphyxial-CA led to transient dysregulation, manifested by velocity significantly impacted MAP. Hyperemia is...
Endocan is thought to be a novel inflammatory marker that associated with variety of diseases. However, its role in the pathogenesis COPD remains unclear. This study aims explore potential endocan COPD.In total, 27 healthy volunteers, 55 patients and 36 acute exacerbation chronic obstructive pulmonary disease (AECOPD) were included study. Basic demographic characteristics, clinical features blood samples collected. Magnetic luminex screening assays used detect concentration endocan, Fas...
Abstract Older patients with traumatic brain injury (TBI) have higher mortality and poorer long-term outlook compared to younger individuals. This may contribute the assumption that aggressive management of geriatric TBI is futile. The present study examined recovery potential underlying mechanisms associated advanced age in male C57BL/6 mice using a controlled cortical impact model TBI. (18 mos) had (10 wks) at 12 weeks post-injury. While aging alone profound on behavioral ability, slope...