A5032932514- Functional Brain Connectivity Studies
- Advanced Neuroimaging Techniques and Applications
- Neurological Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Advanced MRI Techniques and Applications
- Medical Imaging Techniques and Applications
- Neuroscience and Neural Engineering
- Nuclear Receptors and Signaling
- Schizophrenia research and treatment
- 3D Printing in Biomedical Research
- Barrier Structure and Function Studies
- Renin-Angiotensin System Studies
- Biosensors and Analytical Detection
- Dementia and Cognitive Impairment Research
- Photoacoustic and Ultrasonic Imaging
- Genetic Neurodegenerative Diseases
- Cell Image Analysis Techniques
- Treatment of Major Depression
- Mesenchymal stem cell research
- Neuroethics, Human Enhancement, Biomedical Innovations
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Radiomics and Machine Learning in Medical Imaging
McGill University
2015-2024
Montreal Neurological Institute and Hospital
2015-2023
Hospital General Universitario Gregorio Marañón
2013-2016
Centro de Investigación Biomédica en Red de Salud Mental
2013-2016
Universidad Carlos III de Madrid
2013-2016
The purpose of this study was to elucidate whether cerebral blood flow (CBF) can better characterize perfusion abnormalities in predementia stages Alzheimer's disease (AD) than volume (CBV) and cortical atrophy is more associated with decreased CBV or CBF. We compared measurements CBV, CBF, mean thickness obtained from magnetic resonance images a group healthy controls, patients mild cognitive impairment (MCI) who converted AD after 2 years clinical follow-up (MCI-c), AD. A significant...
Brain imaging techniques that use vascular signals to map changes in neuronal activity rely on the coupling between electrophysiology and hemodynamics, a phenomenon referred as “neurovascular coupling” (NVC). It is unknown whether this relationship remains reliable under altered brain states associated with acetylcholine (ACh) levels, such attention arousal pathological conditions Alzheimer's disease. We therefore assessed effects of varying ACh tone whisker-evoked NVC responses rat barrel...
Brain volume and thickness abnormalities have been reported in first-episode psychosis (FEP). However, it is unclear if how they are modulated by brain developmental stage (and, therefore, age at FEP as a proxy). This multicenter cross-sectional case-control magnetic resonance imaging (MRI) study. Patients with ( n = 196), 65.3% males, wide span (12–35 y), healthy controls (HC) 157), matched for age, sex, handedness, were scanned 6 sites. Gray matter measurements generated several regions...
The angiotensin receptor blocker losartan mitigated cerebrovascular and cognitive deficits in mouse models of Alzheimer disease, line with some clinical evidence reduced onset progression to disease. We investigated whether these benefits apply another blocker, namely candesartan. Adult transgenic mice overexpressing a mutated form the human APP (amyloid precursor protein) wild-type controls were treated vehicle or candesartan (cohort 1: 2 months, 1 mg/kg per day, osmotic subcutaneous...
Abstract White matter (WM) pathology is a clinically predictive feature of vascular cognitive impairment and dementia (VCID). Mice overexpressing transforming growth factor‐β1 (TGF) with an underlying cerebrovascular when fed high cholesterol diet (HCD) develop deficits (VCID mice) that we recently found could be prevented by physical exercise (EX). Here, further investigated WM in VCID mice examined the cellular substrates protective effects moderate aerobic EX focusing on alterations. Six...
Antihypertensive medications targeting the renin-angiotensin system have lowered incidence and progression of Alzheimer disease. Understanding how these function could lead to novel therapeutic strategies. AT4Rs (angiotensin IV receptors) been associated with angiotensin receptor blockers' cognitive, cerebrovascular, neuroinflammatory rescue in disease models. Yet, whether act alone or AT2Rs remains unknown. Here, we investigated contribute losartan's benefits chronic AT2R activation mimic...
Inward rectifier potassium (KIR ) channels are key effectors of vasodilatation in neurovascular coupling (NVC). KIR expressed cerebral endothelial cells (ECs) have been confirmed as essential modulators NVC. Alzheimer's disease (AD) and cerebrovascular (CVD) impact on EC-KIR channel function, but whether oxidative stress or inflammation explains this impairment remains elusive.We evaluated function intact EC-denuded pial arteries wild-type (WT) transgenic mice overexpressing a mutated form...
By providing a three-dimensional in vitro culture system with key features of the substantia nigra region brain, 3D neuronal organoids derived from human induced pluripotent stem cells (iPSCs) provide living tissue resembling midbrain brain. However, major limitation conventional brain organoid is that it often labor-intensive, requiring highly specialized personnel for moderate throughput. Additionally, methods published long-term cultures require time-consuming maintenance to generate...
According to the so-called disconnection hypothesis, loss of synaptic inputs from medial temporal lobes (MTL) in Alzheimer's disease (AD) may lead reduced activity target neurons cortical areas and, consequently, decreased cerebral blood flow (CBF) those areas. The aim this study was assess whether hypoperfusion parietotemporal and frontal cortices patients with mild cognitive impairment who converted AD (MCI-c) is associated atrophy MTL and/or microstructural changes white matter (WM)...
Abstract The USP19 deubiquitinase is found in a locus associated with Parkinson’s Disease (PD), interacts chaperonins, and promotes secretion of α-synuclein (α-syn) through the misfolding-associated protein (MAPS) pathway. Since these processes might modulate processing α-syn aggregates PD, we inactivated (KO) mice expressing A53T mutation whom preformed fibrils (PFF) had been injected striatum. Compared to WT, KO brains showed decreased accumulation phospho-synuclein (pSyn) positive...
The cerebellum is the region most commonly used as a reference when normalizing intensity of perfusion images acquired using magnetic resonance imaging (MRI) in Alzheimer's disease (AD) studies. In addition, provides unbiased estimations with nuclear medicine techniques. However, no reports confirm an optimal MRI studies or evaluate consequences different normalization regions. this study, we address effect cerebellum, whole-brain white matter, and cortical gray matter cerebral blood flow...
Inflammatory processes in the brain are orchestrated by microglia and astrocytes response to activators such as pathogen-associated molecular patterns, danger-associated patterns some nanostructures. Microglia primary immune responders initiate responses amplified through intercellular signaling. Intercellular communication between neural cells can be studied cerebral organoids, co-cultures or vivo. We used human organoids glioblastoma study glia modulation dendritic polyglycerol sulfate...
A vascular insult occurring early in disease onset may initiate cognitive decline leading to dementia, while pharmacological and lifestyle interventions can prevent this progression. Mice with a selective, tamoxifen-inducible deletion of NF-κB essential modulator (Nemo) brain endothelial cells were studied as model impairment. Groups included Nemo
Abstract Parkinson's disease is a devastating neurodegenerative disorder characterized by loss of neuromelanin‐containing dopaminergic neurons. Novel 3D culture systems like human midbrain‐like organoids (hMOs) enable new research avenues for patient‐specific therapies, but cannot reach their full potential unless rapid optical imaging entire enabled. Currently, hMOs have to undergo tissue clearing processes before overcome light scattering. Since lengthy chemical procedure, large ensemble...
Optical imaging of human midbrain organoids in the field Parkinson research is challenging using current established technologies since they require lengthy and invasive clearing methods, which preclude live organoid observations longitudinal studies. <br/> Raster-Scanning Optoacoustic Mesoscopy (RSOM) overcomes this limitation by utilizing intrinsic optical absorption contrast based on optoacoustic effect. We show that RSOM able to provide fast quantitative visualization neuromelanin...
ABSTRACT By providing a three-dimensional in vitro culture system with key features of the substantia nigra region brain, 3D neuronal organoids derived from human induced pluripotent stem cells (iPSCs) provide living tissue resembling midbrain brain. However, major limitation conventional brain organoid is that it often labor-intensive, requiring highly specialized personnel for moderate throughput. Additionally, methods published long-term cultures require time-consuming maintenance to...
Over the past few decades, scientists have transformed way they do and understand science. They are now exploring their more creative side. This approach has accelerated fascinating discoveries such as human induced pluripotent stem cells brain organoids; however, not been able to jump over communication barrier with society. La Danse des Astrocytes, a scene observed during routine microscopy session working on midbrain organoids, motivated this essay, which urges find new forms of science...