Objective. To project future total hip and knee joint arthroplasty (THA, TKA) use in the United States to 2040. Methods. We used 2000–2014 US National Inpatient Sample (NIS) combined with Census Bureau data develop projections for primary THA TKA from 2020 2040 using polynomial regression account nonlinearity interactions between variables, assuming underlying distribution of number THA/TKA be Poisson distributed. performed sensitivity analyses a negative binomial overdispersion. Results....

Abstract Many tumor cells shed specialized membrane vesicles known as exosomes. In this study, we show that pretreatment of mice with exosomes produced by TS/A or 4T.1 murine mammary resulted in accelerated growth implanted both syngeneic BALB/c and nude mice. As grew more rapidly had been depleted NK cells, analyzed the effects tumor-derived on cells. The inhibit cell cytotoxic activity ex vivo vitro demonstrated chromium release assays. treatment also led to a reduction percentages...

Abstract The production of exosomes by tumor cells has been implicated in tumor-associated immune suppression. In this study, we show that, mice, produced TS/A murine mammary target CD11b+ myeloid precursors the bone marrow (BM) vivo, and that is associated with an accumulation spleen. Moreover, demonstrate block differentiation precursor into dendritic (DC) vitro. Addition at day 0 led to a significant DC, whereas addition later time points was less effective. Similarly, human breast...

Abstract In common with many other cell types, synovial fibroblasts produce exosomes. this study, we show that the exosomes produced by obtained from individuals rheumatoid arthritis (RASF), but not osteoarthritis, contain a membrane bound form of TNF-α as demonstrated colloidal gold immunostaining and confirmed both Western blot mass spectrometry. The RASF-derived exosomes, derived are cytotoxic for L929 cell, TNF-α-sensitive line, stimulate activation NF-κB induction collagenase-1 in RASF....

Background Emergency medical service (EMS) personnel have high COVID-19 risk during resuscitation. The resuscitation protocol for patients with out-of-hospital cardiac arrest (OHCA) was modified in response to the pandemic. However, how adjustments EMS system affected OHCA remains unclear. Methods We analysed data from Taichung registry system. compared outcomes and rescue records 622 cases outbreak period (1 February 30 April 2020) those recorded 570 same 2019. Results two periods did not...

Few studies, if any, have examined cardiovascular outcomes in patients with diabetes and gout. Both gout are risk factors for disease. The objective of this study was to examine the effect allopurinol on incident acute events diabetes. We used 2007–2010 Multi-Payer Claims Database (MPCD) that linked health plan data from national commercial governmental insurances, representing beneficiaries United Healthcare, Medicare, or Medicaid coverage. In diabetes, we assessed current use, defined as a...

Parkinson's disease (PD) is the second most common age-related neurodegenerative disorder with limited clinical treatments. The occurrence of PD includes both genetic and environmental toxins, such as pesticides paraquat (PQ), major contributors to pathology in invertebrate mammalian models. Calycosin, an isoflavone phytoestrogen, has multiple pharmacological properties, including neuroprotective activity. However, paucity information regarding potential calycosin on PQ-induced...

Previously, we described an APC-adenovirus (APC-Ad) FasL cell gene therapy method which could be used to deplete autoreactive T cells in vivo. was toxic, however, and controlled regulation of not achieved. Here describe improved approach delivering TNF-related apoptosis-inducing ligand (TRAIL) vivo collagen II–induced (CII-induced) arthritis–susceptible (CIA-susceptible) DBA/1j mice were treated with CII-pulsed DCs that had been transfected a novel Ad system. The engineered exhibit inducible...

Previously, we described an APC-adenovirus (APC-Ad) FasL cell gene therapy method which could be used to deplete autoreactive T cells in vivo. was toxic, however, and controlled regulation of not achieved. Here describe improved approach delivering TNF-related apoptosis-inducing ligand (TRAIL) vivo collagen II–induced (CII-induced) arthritis–susceptible (CIA-susceptible) DBA/1j mice were treated with CII-pulsed DCs that had been transfected a novel Ad system. The engineered exhibit inducible...

Radiation exposure from a number of terrestrial sources is associated with an increased risk for atherosclerosis. Recently, concern over whether to cosmic radiation might pose similar astronauts has increased. To address this question, we examined the effect 2 5 Gy iron ions ((56)Fe), particularly damaging component radiation, targeted specific arterial sites in male apolipoprotein E-deficient (apoE(-/-)) mice. accelerated development atherosclerosis irradiated portions aorta independent any...

We recently identified Smads1, 5 and 8 as muscle biomarkers in human ALS. In the ALS mouse, these markers are elevated track disease progression. Smads signal transducers become activated upon receptor engagement of ligands from TGF-β superfamily. Here, we sought to characterize linked activation their role RNA sequencing data samples were mined for superfamily ligands. Candidate targets validated by qRT-PCR a large cohort biopsy G93A SOD1 mouse. Protein expression was evaluated Western...

Previous observational studies that have examined the association of allopurinol with myocardial infarction (MI) provided contradictory results. One study showed reduced risk, while another an increased risk allopurinol. Therefore, our objective was to assess whether use is associated a reduction in MI elderly.We used 2006-2012 5 % random sample Medicare beneficiaries new initiation and incident cohort study. Multivariable-adjusted Cox regression models adjusted for age, gender, race,...

Abstract Objective To determine novel genes regulated by tumor necrosis factor α (TNFα) signaling in primary rheumatoid arthritis synovial fibroblasts (RASFs). Methods Oligonucleotide microarrays were used to measure gene expression levels 6 independent replicate samples of RASFs. RASFs transfected for 18 hours with AdIκB–dominant negative (AdIκB‐DN) (n = 3) or control AdTet expressing the reverse tetracycline trans ‐activator 3). The cells stimulated 3 TNFα, and total RNA was prepared....

Objective To assess the health care burden of septic arthritis in U.S. and examine associated factors. Methods We used Nationwide Emergency Department Sample (NEDS) data patients hospitalized with as primary diagnosis from 2009–12 to time-trends. Multivariable-adjusted models assessed demographics, comorbidity hospital characteristics potential predictors duration hospitalization, total (inpatient ED) charges discharge home. Results In 2009, 2010 2012 U.S., respectively, there were 13,087,...

Previous studies of allopurinol and stroke risk have provided contradictory findings, ranging from a protective effect to an increased risk. Our objective was assess whether use is associated with the in elderly. We used 5 % random sample Medicare beneficiaries 2006–2012 study association new initiation incident stroke. multivariable-adjusted Cox regression models adjusted for age, gender, race, Charlson index, cardio-protective medications (beta-blockers, ACE inhibitors, diuretics, statins)...

Gout is a risk factor for cardiovascular disease, but associations with specific outcomes, myocardial infarction (MI), and stroke are unclear. Our objective in the present study was to assess whether gout as strong diabetes mellitus (DM) incident MI stroke. In this retrospective study, we used U.S. claims data from 2007 2010 that included mix of private public health plans. Four mutually exclusive cohorts were identified: (1) DM only, (2) (3) DM, (4) neither nor DM. Outcomes acute or...

Objective To assess the health care utilization and emergency department ( ED ) charges for septic arthritis. Methods We performed a retrospective cohort study of visits with arthritis as primary diagnosis using US Nationwide Emergency Department Sample data from 2009–2012. examined charges, hospitalization, associated time trends. Multivariable models were adjusted demographics (age, sex, insurance status, residence, household income), comorbidity, hospital characteristics to factors...

To assess the effect of allopurinol use on risk incident atrial fibrillation (AF) in elderly.We used 5% random Medicare Claims data from 2006 to 2012 examine association and AF a cohort patients with an absence at baseline (at least 365 days). Multivariable-adjusted Cox regression analyses compared exposed non-exposed periods for AF, controlling age, sex, race, Charlson-Romano comorbidity index statins, diuretics, ACE inhibitors β-blockers. HR 95% CIs was calculated. Sensitivity considered...