A5052546557- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Reproductive Biology and Fertility
- Renal and related cancers
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Pluripotent Stem Cells Research
- Genetic Syndromes and Imprinting
- Nuclear Structure and Function
- Prenatal Screening and Diagnostics
- Cancer-related gene regulation
- dental development and anomalies
Kumamoto University
2020-2022
RIKEN Center for Integrative Medical Sciences
2012-2021
Kyushu University
2021
Epigenetic modifications influence gene expression and chromatin remodeling. In embryonic pluripotent stem cells, these epigenetic have been extensively characterized; by contrast, the events of tissue-specific cells are poorly understood. Here, we define a new shift that is crucial for differentiation murine spermatogonia toward meiosis. We exploited property incomplete cytokinesis, which causes male germ to form aligned chains characteristic lengths, as they divide differentiate. These...
Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I. initiated by DNA double-strand break (DSB) followed multiple processes repair. The exact mechanisms how recombinases localize to DSB remain elusive. Here, we show C19orf57/4930432K21Rik/BRME1 a player meiotic in mice. associates with single-stranded (ssDNA) binding proteins, BRCA2 MEILB2/HSF2BP, which are recruiters onto sites. Disruption shows...
During meiotic prophase, sister chromatids are organized into axial element (AE), which underlies the structural framework for events such as recombination and homolog synapsis. HORMA domain-containing proteins (HORMADs) localize along AE play critical roles in regulation of those events. Organization is attributed to two groups proteins: cohesins REC8 RAD21L; components SYCP2 SYCP3. It has been elusive how these chromosome contribute chromatin loading HORMADs prior formation. Here we newly...
Abstract During spermatogenesis, meiosis is accompanied by a robust alteration in gene expression and chromatin status. However, it remains elusive how the meiotic transcriptional program established to ensure completion of prophase. Here, we identify protein complex that consists germ-cell-specific zinc-finger ZFP541 its interactor KCTD19 as key regulators mouse prophase progression. Our genetic study shows are co-expressed from pachytene onward play an essential role testis. Furthermore,...
Meiosis is a cell division process with complex chromosome events where various molecules must work in tandem. To find meiosis-related genes, we screened evolutionarily conserved and reproductive tract-enriched genes using the CRISPR/Cas9 system identified potassium channel tetramerization domain containing 19 ( Kctd19 ) as an essential factor for meiosis. In prophase I, deficiency did not affect synapsis or DNA damage response, chiasma structures were also observed metaphase I spermatocytes...
Meiotic prophase I is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic I, homologous chromosomes undergo synapsis to facilitate recombination yielding crossovers. It remains largely elusive how homolog temporally maintained and destabilized during I. Here we show FBXO47 stabilizer synaptonemal complex male Disruption shows severe impact on synapsis, recombination, XY body formation, leading infertility. Notably, in absence...
Heterochromatin-related epigenetic mechanisms, such as DNA methylation, facilitate pairing of homologous chromosomes during the meiotic prophase mammalian spermatogenesis. In pro-spermatogonia, de novo methylation plays a key role in completing and initiating division. However, maintenance regulation meiosis, especially adult, is not well understood. Here, we reveal that NP95 (also known UHRF1) DNMT1 - two essential proteins for are co-expressed spermatogonia necessary meiosis male germ...
Summary Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I. initiated by DNA double-strand break (DSB) followed multiple processes repair. The exact mechanisms how recombinases localize to DSB remained elusive. Here we show C19orf57/4930432K21Rik/BRME1 a new player meiotic in mice. associates with ssDNA binding proteins, BRCA2 MEILB2/HSF2BP, recruiters onto sites. Disruption shows severe impact on...
Abstract Meiosis is a cell division process with complex chromosome events where various molecules must work in tandem. To find meiosis-related genes, we screened evolutionarily conserved and reproductive tract-enriched genes using the CRISPR/Cas9 system identified potassium channel tetramerization domain containing 19 ( Kctd19 ) as an essential factor for meiosis. In prophase I, deficiency did not affect synapsis or DNA damage response, chiasma structures were also observed metaphase I...
Abstract Meiotic prophase is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic prophase, homologous chromosomes undergo synapsis to facilitate recombination yielding crossovers. It remains largely elusive how homolog temporally maintained and destabilized during prophase. Here we show FBXO47 stabilizer synaptonemal complex male Disruption shows severe impact on DSB repair processes, leading infertility. Notably, in absence FBXO47,...
Meiotic prophase is a prolonged G2 phase that ensures the completion of numerous meiosis-specific chromosome events. During meiotic prophase, homologous chromosomes undergo synapsis to facilitate recombination yielding crossovers. It remains largely elusive how homolog temporally maintained and destabilized during prophase. Here we show FBXO47 stabilizer synaptonemal complex male Disruption shows severe impact on DSB repair processes, leading infertility. Notably, in absence FBXO47, although...