A5000275665- Marine Toxins and Detection Methods
- Neuroscience of respiration and sleep
- Forensic Toxicology and Drug Analysis
- Marine and coastal ecosystems
- Marine Invertebrate Physiology and Ecology
- Nicotinic Acetylcholine Receptors Study
- Venomous Animal Envenomation and Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Environmental Toxicology and Ecotoxicology
- Marine Sponges and Natural Products
- Identification and Quantification in Food
- Vibrio bacteria research studies
- Animal Ecology and Behavior Studies
- Marine and environmental studies
- Marine Biology and Ecology Research
- Cassava research and cyanide
- Metabolomics and Mass Spectrometry Studies
- Ion channel regulation and function
- Biocrusts and Microbial Ecology
- Pesticide Residue Analysis and Safety
- Biopolymer Synthesis and Applications
- Polyamine Metabolism and Applications
- Nitric Oxide and Endothelin Effects
- Redox biology and oxidative stress
- Botany and Plant Ecology Studies
National Institute of Health Sciences
2013-2024
Xiamen University Malaysia
2021
UCSI University
2021
National Institute of Health Sciences
2016
Okinawa Prefectural Institute of Health and Environment
2002-2013
Tohoku University
2010
Tokyo University of Marine Science and Technology
2009
University of the Ryukyus
2003
Toxin profiles of representative ciguatera species caught at different locations Japan were investigated in fish flesh by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Identification and quantification 16 toxins facilitated the use 14 reference prepared either synthesis or isolation from natural sources previous LC-MS data thereof. Sodium adduct ions [M + Na](+) used as parent product ions. Distinct regional differences unveiled: ciguatoxin-1B type...
Ciguatoxins (CTXs) contaminate fish worldwide and cause the foodborne illness ciguatera. In Pacific, these toxins are produced by dinoflagellate Gambierdiscus toxicus, which accumulates in through food chain undergoes oxidative modification, giving rise to numerous analogs. this study, we examined oxidation of CTXs vitro with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using reference toxins, found that CTX4A, CTX4B, CTX3C, alga, oxidized analogs fish, namely CTX1B,...
Abstract Ciguatera fish poisoning is the most extensive and difficult to control of seafood poisonings. Tofacilitate monitoring toxicity, toxin profiles were investigated by an LC/MS/MS method using 14reference toxins on eight representative species collected in four different areas Pacific. Snappers groupers from Okinawa contained ciguatoxin-1B (CTX1B) two deoxy congeners at variablebut species-specific ratios, while red snapper, Lutjanus bohar, Minamitorishima, amberjack, Seriola dumerili,...
The deadly box jellyfish (Sea Wasp, Habu-kurage in Japanese) Chiropsalmus quadrigatus Haeckel (Cubozoa) is distributed widely the tropical Pacific region. In Japan, three fatal cases due to stings from this species have been reported officially. We successfully isolated C. toxin-A (CqTX-A, 44 kDa), a major proteinaceous toxin, for first time, nematocysts of quadrigatus. CqTX-A showed lethal toxicity crayfish when administered via intraperitoneal injection (LD50 = 80 microg/kg) and hemolytic...
Ciguatera fish poisoning (CFP) is one of the most frequently reported seafood diseases. It endemic to tropical region and occurs commonly in regions around Pacific Ocean, Indian Caribbean Sea. The principal toxins causing CFP are ciguatoxins (CTXs). In region, more than 20 analogs CTXs have been identified date. Based on their skeletal structures, they classified into CTX1B-type CTX3C-type toxins. We previously species-specific regional-specific toxin profiles. this study, levels profiles...
Ciguatera fish poisoning (CFP) is one of the most frequently encountered seafood syndromes; it caused by consumption marine finfish contaminated with ciguatoxins (CTXs). The majority CFP cases result from eating flesh, but a traditional belief exists among people that head and viscera are more toxic should be avoided. Unlike viscera, scientific data to support legendary high toxicity scarce. We prepared tissue samples fillet, head, eyes taken five yellow-edged lyretail (Variola louti)...
Ingesting fish contaminated with ciguatoxins (CTXs) originating from epibenthic dinoflagellates causes ciguatera poisoning (CFP). CFP occurs mainly in the tropical and subtropical Indo–Pacific region Caribbean Sea. Furthermore, it sporadically Japan, especially Ryukyu Islands between Taiwan Kyushu, Japan. Variola louti is most frequently implicated a suggested toxin profile, consisting of ciguatoxin-1B two deoxy congeners. Therefore, using liquid chromatography–tandem mass spectrometry...
Okadaic acids (OAs) are causative agents of diarrhetic shellfish poisoning, produced by the dinoflagellates Dinophysis spp. and Prorocentrum Microcystins (MCs) cyclic heptapeptide hepatotoxins some cyanobacteria genera, including Microcystis Traditionally, toxicity detection quantification these natural toxins were performed using a mouse bioassay (MBA); however, this is no longer widely employed owing to its lack accuracy, sensitivity, with regard animal welfare. Therefore, alternative...
Ciguatera fish poisoning (CFP) refers to an illness caused by ingesting that have accumulated ciguatoxins (CTXs). CFP frequently occurs in the tropical and subtropical Indo-Pacific Ocean Caribbean Sea. In Japan, sporadically but constantly Okinawa Amami Islands. The grouper Variola albimarginata is regarded be safe for consumption. To assess real risk of V. albimarginata, we analyzed 133 specimens using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Ciguatoxin-1B,...
The venomous sea anemone Phyllodiscus semoni causes cases of severe stinging. We isolated toxin 20A (PsTX-20A), a hemolytic and lethal polypeptide (20 kDa), from the nematocyst venom this species for first time. Furthermore, we sequenced cDNA encoding PsTX-20A. deduced amino acid sequence PsTX-20A showed that was new member actinoporin family, which consists several cytolytic polypeptides originating anemones. toxicity to shrimp Palaemon paucidens when administered via intraperitoneal...
2008年に鹿児島県奄美群島で発生した魚類摂食に起因する食中毒シガテラの3事例について,原因魚3試料および同時に漁獲された近海魚5種7試料のLC-MS/MSによるシガトキシン類(CTXs)一斉分析の検討を行った.食中毒の原因となったイッテンフエダイ2試料およびバラハタ1試料のすべてからCTX1B,54-deoxyCTX1B,52-epi-54-deoxyCTX1Bが検出されたが,CTX3C類縁体は認められなかった.これら2魚種のCTXs組成比は,それぞれ沖縄海域の両種における組成と共通していた.一方,宮崎県での食中毒原因試料はCTX3C類縁体が主要毒であり,毒組成の違いが示された.また,LC-MS/MSで測定した毒量はマウス毒性試験(MBA)結果(0.1~0.8 MU/g)と同程度であったが,比毒性が明確でない54-deoxyCTX1Bによる総毒力への影響も推察された.一方,MBAで陰性(<0.025 MU/g)を示した近海魚1試料からも微量のCTXsが検出された.
Ciguatera fish poisoning (CFP) poses a serious threat to both public health and the use of aquatic resources from various warm-water regions world. Hence, process for efficient determination relevant toxins is required.We sought develop validate first LC-MS/MS method quantify major prevalent in Pacific Ocean.Toxins were extracted flesh (2 g) using methanol-water mixture (9:1, v/v). The extract was heated at 80°C, low-polarity lipids eliminated hexane, initially basic solution later acidic...
Natto and miso are two major traditional fermented soy foods in Japan. is prepared by fermenting cooked soybeans with Bacillus subtilis natto. The beans of natto have a sticky outlook, slippery texture, sour aroma nutty flavour. Bioactive components nattokinase, bacillopeptidase F, vitamin K2, dipicolinic acid γ-polyglutamic acid. Miso soybean paste widely used to make soup. produced koji (steamed rice inoculated Aspergillus oryzae). compounds include isoflavones phenolic acids. In this...
Marine biotoxins refer to bioactive natural products primarily produced by microalgae and bacteria may affect aquatic organisms human health [...]
世界最大規模の自然毒食中毒シガテラの未然防止のために,地方自治体では可能性のある魚種を指定して販売自粛を指導している.水産卸売市場で販売が自粛された魚類7種18試料についてLC-MS/MSによりシガトキシン類(CTXs)を分析した結果,5試料(バラフエダイ4試料およびバラハタ1試料)からCTXsが検出された.含量の高かった2試料(No. 5: 0.348 μg/kg, No. 8: 0.362 μg/kg)は200 g程度の摂食で発症すると推定され,販売自粛がCFPを未然に防止したことが示唆された.産地不明のバラフエダイ(1試料)からはCTX1B系列(CTX1B, 52-epi-54-deoxyCTX1Bおよび54-deoxyCTX1B)のみが検出され,沖縄・奄美産バラフエダイと組成が類似していることから沖縄・奄美海域で漁獲された可能性が示唆される.一方,和歌山産バラフエダイ(2試料)からはCTX1B系列とCTX3C系列(2,3-dihydroxyCTX3C, 2,3,51-trihydroxyCTX3C,...