A5059363444- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Lipid metabolism and biosynthesis
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Botanical Studies and Applications
- Plant Genetic and Mutation Studies
- Phytochemical and Pharmacological Studies
- Plant Molecular Biology Research
- Food composition and properties
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Bioenergy crop production and management
- Diet, Metabolism, and Disease
- Plant Virus Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Plant Diversity and Evolution
- Genetic diversity and population structure
- Plant pathogens and resistance mechanisms
- Medicinal plant effects and applications
- Plant and animal studies
Lomonosov Moscow State University
2020-2025
Engelhardt Institute of Molecular Biology
2022-2025
FAD (fatty acid desaturase) and SAD (stearoyl-ACP genes play key roles in the synthesis of fatty acids (FA) determination oil composition flax (Linum usitatissimum L.). We searched for most widely used genome variety CDC Bethune three available long-read assembled genomes-YY5, 3896, Atlant. identified fifteen FAD2, six FAD3, four genes. Of all genes, 24 were present duplicated pairs. In cases, two from a pair differed by significant number gene-specific SNPs (single nucleotide polymorphisms)...
Flax seed is one of the richest plant sources linolenic acid (LIN) and also contains unsaturated linoleic (LIO) oleic (OLE). Stearoyl-ACP desaturases (SADs) fatty (FADs) play key roles in synthesis flax acids (FAs). However, there no holistic view which genes from SAD FAD families at developmental stages have highest expression levels seeds, as well influence genotype growth conditions on profiles these genes. We sequenced transcriptomes 3, 7, 14, 21, 28 days after flowering (DAF) for ten...
1IntroductionSea buckthorn (Hippophae rhamnoides L.) is a woody oil tree known for its fruits, which are rich source of bioactive compounds, including carotenoids and flavonoids (Ciesarova et al., 2020; Mihal 2023). In addition, the unique fatty acid composition fruit pulp oil, especially high content omega-7 monounsaturated palmitoleic acid, rare in plants, contributes to nutritional benefits products (Sola Marsinach Cuenca, 2019). this regard, sea used medicine, cosmetics, nutraceuticals...
Flax (Linum usitatissimum L.) is attacked by numerous devastating fungal pathogens, including Colletotrichum lini, Aureobasidium pullulans, and Fusarium verticillioides (Fusarium moniliforme). The effective control of flax diseases follows the paradigm extensive molecular research on pathogenicity. However, such studies require quality genome sequences studied organisms. This article reports approaches to assembling a high-quality from Oxford Nanopore Technologies data. We sequenced genomes...
High-quality genome sequences help to elucidate the genetic basis of numerous biological processes and track species evolution. For flax (Linum usitatissimum L.)—a multifunctional crop, high-quality assemblies from Oxford Nanopore Technologies (ONT) data were unavailable, largely due difficulty isolating pure high-molecular-weight DNA. This article proposes a scheme for gaining contiguous L. assembly using data. We developed protocol nuclei isolation with subsequent DNA extraction, which...
Colletotrichum lini is a flax fungal pathogen, comprising differently virulent strains. In this work, we sequenced the genomes of three C. strains different virulence: high #390-1, medium #757, and low #771. We obtained more than 100× genome coverage with Oxford Nanopore Technologies reads 50× Illumina data. To construct high-quality genomes, several assembly strategies were tested. The final assemblies using Canu – Racon ×2 Medaka Polca scheme 54.0-55.3 Mb in length, contained 26-32...
is a fungal pathogen of flax that can cause significant yield and quality losses. In this work, we obtained the first complete annotated genome assembly highly virulent
Flax is an important crop grown for seed and fiber. chromosome number 2n = 30, its genome size about 450–480 Mb. To date, the genomes of several flax varieties have been sequenced assembled. However, obtained assemblies are still far from telomere-to-telomere (T2T) level. We variety K-3018 on Oxford Nanopore Technologies (ONT) platform 57.7 Gb R10 simplex reads with N50 18.4 kb (~120× coverage). ONT longer than 50 were kept as ultra-long ones (~10× coverage), rest corrected using HERRO model...
KAS II (β-ketoacyl-acyl carrier protein (ACP) synthases II), FAT (fatty acid thioesterases), SAD (stearoyl-ACP desaturase), and FAD desaturases) are the vital gene families involved in fatty (FA) synthesis Hippophae rhamnoides L. However, information on number location of these genes which ones key to formation FAs fruit seeds pulp was not complete. Our study aimed solve this issue using available genomic sequences transcriptome data that we obtained. We compared sea buckthorn with those...
Sequencing whole plant genomes provides a solid foundation for applied and basic studies. Genome sequences of agricultural plants attract special attention, as they reveal information on the regulation beneficial traits. Flax is valuable crop cultivated oil fiber. its representatives are rich sources genetic improvement forms plant. In our work, we sequenced first genome flax with dehiscence capsules-Linum usitatissimum convar. сrepitans (Boenn.) Dumort-on Oxford Nanopore Technologies (ONT)...
Members of the genus Populus L. play an important role in formation forests northern hemisphere and are used urban landscaping timber production. species closely related sections show extensive hybridization. Therefore, systematics is rather complicated, especially for poplars hybrid origin. We aimed to assess efficiency application sex-determining region (SDR) addition nuclear chloroplast genome loci traditionally phylogenetic studies investigate relationships Aigeiros Duby Tacamahaca...
Flax (Linum usitatissimum L.) products are used in the food, pharmaceutical, textile, polymer, medical, and other industries. The creation of a pan-genome will be an important advance flax research breeding. selection genotypes that sufficiently cover species diversity is crucial step for pan-genomic study. For this purpose, we have adapted method based on Illumina sequencing transcriptome libraries prepared using Tn5 transposase (tagmentase). This approach reduces cost sample preparation...
Flax (Linum usitatissimum L.), one of the important and versatile crops, is used for production oil fiber. To obtain high stable yields flax products, L. varieties should be cultivated under optimal conditions, including composition soil microbiome. We evaluated diversity microorganisms in soils conditions unfavorable cultivation (suboptimal acidity or herbicide treatment) infected with causative agents harmful diseases (Septoria linicola, Colletotrichum lini, Melampsora Fusarium oxysporum...
Flax seed is one of the richest plant sources linolenic acid (LIN) and also contains unsaturated linoleic (LIO) oleic (OLE). Stearoyl-ACP desaturases (SAD) fatty (FAD) play a key role in synthesis flax acids (FA). We sequenced transcriptomes at 3, 7, 14, 21, 28 day after flowering (DAF) for ten varieties with different oil FA compositions grown under three temperature/watering conditions. Expression levels 25 genes SAD, FAD2, FAD3 families were evaluated. FAD3b, FAD3a, FAD2b-2, SAD3-1,...
Colletotrichum lini is a flax fungal pathogen. The genus comprises differently virulent strains, leading to significant yield losses. However, there were no attempts investigate the molecular mechanisms of C. pathogenicity from high-quality genome assemblies until this study. In work, we sequenced genomes three strains high (#390-1), medium (#757), and low (#771) virulence. We obtained more than 100× coverage with Oxford Nanopore Technologies reads (N50 = 12.1, 6.1, 5.0 kb) 50× Illumina data...
Colletotrichum lini is a pathogenic fungus that infects flax and causes significant yield losses. In this study, we assembled the genomes of four highly virulent C. strains using Oxford Nanopore Technologies (ONT, R10.4.1 flow cells) Illumina platforms. The performance two tools developed for telomere-to-telomere (T2T) genome assembly was compared: Verkko Hifiasm. Prior to assembly, ONT reads were corrected HERRO algorithm. generated assemblies high completeness but low contiguity, while...
Abstract Astropanax and Neocussonia are two recently resurrected genera of Araliaceae that had long been considered as an Afro-Malagasy lineage Schefflera. The wood structure 11 spp. eight has studied. shows a higher average length vessel elements (1319 µm) number bars on perforation plates (up to 66) than any other examined date. is distinct from by its smaller diameter frequency vessels, rare occurrence simple plates, more numerous scalariform intervessel pits. interspecific variation in...