A5101619245- Diabetes Treatment and Management
- Toxic Organic Pollutants Impact
- Pharmacogenetics and Drug Metabolism
- Japanese History and Culture
- Diabetes Management and Research
- Probiotics and Fermented Foods
- Metabolism, Diabetes, and Cancer
- Environmental Toxicology and Ecotoxicology
- Pharmacology and Obesity Treatment
- Effects and risks of endocrine disrupting chemicals
- Immune Cell Function and Interaction
- Neuroendocrine Tumor Research Advances
- Zebrafish Biomedical Research Applications
- T-cell and B-cell Immunology
- Gut microbiota and health
- Pancreatic function and diabetes
- Asthma and respiratory diseases
- Pregnancy and preeclampsia studies
- Hormonal and reproductive studies
- Drug Transport and Resistance Mechanisms
- Estrogen and related hormone effects
- Chinese history and philosophy
- Microbial bioremediation and biosurfactants
- Monoclonal and Polyclonal Antibodies Research
- Head and Neck Cancer Studies
Obihiro University of Agriculture and Veterinary Medicine
2015-2024
Kanagawa Prefecture Medical Association
2012-2021
Fujieda Municipal General Hospital
2020
Megmilk Snow Brand (Japan)
2007-2016
Woods Hole Oceanographic Institution
2010-2016
Rakuno Gakuen University
2008-2014
Kubota (Japan)
2012-2014
Kansai Electric Power Hospital
2014
Ehime University
2004-2013
Hiroshima University
2011-2013
Increasing use of zebrafish in drug discovery and mechanistic toxicology demands knowledge cytochrome P450 (CYP) gene regulation function. CYP enzymes catalyze oxidative transformation leading to activation or inactivation many endogenous exogenous chemicals, with consequences for normal physiology disease processes. Many CYPs potentially have roles developmental specification, chemicals that cause abnormalities are substrates CYPs. Here we identify annotate the full suite genes zebrafish,...
We previously reported the cloning of two distinct somatostatin receptor (SSTR) subtypes, SSTR1 and SSTR2. Although both SSTR2 bound specifically with high affinity, neither was coupled to adenylyl cyclase, a major cellular effector somatostatin's actions. Here we report functional characterization third member SSTR family. Human SSTR3 is protein 418 amino acids has 45% 46% identity human SSTR2, respectively. RNA blotting studies showed that mRNA could be readily detected in brain pancreatic...
Aims: To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model. Methods and Results: After 3 days of exposure to rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR/8/34 (H1N1). Mice treated LGG showed lower frequency accumulated symptoms higher survival rate than control (P < 0·05). The YAC-1 cell-killing activity lung cells isolated was significantly greater...
Abstract Murine NK cytotoxicity is regulated by two families of MHC class I-specific receptors, namely Ly49 and CD94/NKG2. We developed a single-cell RT-PCR method to analyze expression all known NKG2A genes in individual cells determined the receptor repertoires from adult neonatal (1-wk-old) C57BL/6 mice. In mouse cells, up six different receptors were coexpressed random combinations. Of 62 examined, 42 patterns observed. Most them expressed at least one Ly49, whereas was detected 32%...
Aims: Our study was conducted to evaluate the potent protective effects of oral administration probiotic Lactobacillus strains against influenza virus (Flu) infection in a mouse model. Method and Results: Lyophilized rhamnosus GG (LGG) gasseri TMC0356 (TMC0356) were orally administered BALB/c mice for 19 days. The test intranasally infected with Flu A/PR/8/34 (H1N1) on day 14, any changes clinical symptoms monitored. After 6 days infection, killed pulmonary titres determined. symptom scores...
This study investigated whether heat-killed Lactobacillus protects host animal against influenza virus infection and stimulates their immunity. Heat-killed gasseri TMC0356 was orally administered to BALB/c mice for 19 days; the were intranasally infected with Flu A/PR/8/34 (H1N1) on day 14, clinical symptoms monitored. After 6 days, sacrificed, pulmonary titres determined. Splenic activation of natural killer (NK) cells mRNA expression cytokines other immune molecules in lung Peyer's patch...
We have shown recently that oxidative stress by chronic hyperglycemia damages the pancreatic β‐cells of GK rats, a model non‐obese type 2 diabetes, which may worsen diabetic condition and suggested administration antioxidants as supportive therapy. To determine if natural antioxidant α‐tocopherol (vitamin E) has beneficial effects on glycemic control rats were fed diet containing 0, 20 or 500 mg/kg α‐tocopherol. Intraperitoneal glucose tolerance test revealed significant increment insulin...
Ligand-activated receptors regulate numerous genes, and mediate effects of a broad set endogenous exogenous chemicals in vertebrates. Understanding the roles these transcription factors zebrafish (Danio rerio) is important to use this non-mammalian model toxicological, pharmacological, carcinogenesis research. Response potential agonist for pregnane X receptor (Pxr) [pregnenolone (PN)] was examined developing zebrafish, assess involvement Pxr regulation selected including genes cytochrome...
There are growing concerns about the increase in hyperthyroidism pet cats due to exposure organohalogen contaminants and their hydroxylated metabolites. This study investigated blood polychlorinated biphenyls (PCBs) polybrominated diphenyl ethers (PBDEs) methoxylated derivatives (OH-PCBs, OH-PBDEs, MeO-PBDEs), dogs cats. We also measured residue levels of these compounds commercially available foods. Chemical analyses PCBs OH-PCBs showed that OH-PCB were 1 2 orders magnitude lower cat dog...
6-Formylindolo[3,2-b]carbazole (FICZ) is a potent aryl hydrocarbon receptor (AHR) agonist that efficiently metabolized by AHR-regulated cytochrome P4501 enzymes. FICZ proposed physiological AHR ligand induces its own degradation as part of regulatory negative feedback loop. In vitro studies in cells show CYP1 inhibition the presence results enhanced activation, suggesting accumulates cell when metabolism blocked. We used zebrafish (Danio rerio) embryos to investigate vivo effects CYP1A...
In the mouse, two families of MHC class I-specific receptors, namely Ly49 and CD94/NKG2, have been identified on NK cells. Individual cells can express several molecules as well members CD94/NKG2 family. The expression multiple receptors with different specificities for I is thus thought to generate diverse recognition patterns. To delineate mechanism by which begin patterns molecules, we developed a clonal assay in NK1.1(-), IL-2/ IL-15 receptor beta+ precursors generated culture...
In the mouse, two families of MHC class I-specific receptors, namely Ly49 and CD94/NKG2, have been identified on NK cells. Individual cells can express several molecules as well members CD94/NKG2 family. The expression multiple receptors with different specificities for I is thus thought to generate diverse recognition patterns. To delineate mechanism by which begin patterns molecules, we developed a clonal assay in NK1.1(-), IL-2/ IL-15 receptor beta+ precursors generated culture...
Abstract (J Diabetes Invest, doi: 10.1111/j.2040‐1124.2012.00221.x, 2012) Aims/Introduction: To determine the efficacy and safety of sitagliptin monotherapy combination therapy in Japanese type 2 diabetes patients after 3 months’ therapy. Materials Methods: A retrospective, observational study 741 was carried out; 110 received monotherapy, 631 with when other oral medications were insufficient. The primary outcome measure glycated hemoglobin (HbA 1c ) measured at 0, 4 12 weeks Results: In...
There have only been a few reports about use of dipeptidyl peptidase 4 (DPP-4) inhibitors in elderly patients with type 2 diabetes mellitus (T2DM), suggesting that the safety these agents has not sufficiently demonstrated. We performed comparative review efficacy and sitagliptin for Japanese T2DM managed real-world clinical setting. An age-stratified analysis was 831 who were treated years. Parameters assessed included hemoglobin A1c (HbA1c), body weight, serum creatinine, adverse events....
Ipragliflozin is a sodium-glucose co-transporter 2 inhibitor that can improve glycemic control and reduce body weight blood pressure in patients with type diabetes mellitus (T2DM). We evaluated the efficacy safety of ipragliflozin real-world clinical setting, focus on changes composition up to 3 months treatment.This was prospective multicenter interventional trial. investigated pressure, composition, glucose, hemoglobin A1c (HbA1c), ketone bodies, lipids, insulin after treatment (50 - 100...