A5046861121- Wound Healing and Treatments
- Burn Injury Management and Outcomes
- Pressure Ulcer Prevention and Management
- Surgical Sutures and Adhesives
- Planarian Biology and Electrostimulation
- Diabetic Foot Ulcer Assessment and Management
- Bacterial biofilms and quorum sensing
- Reconstructive Surgery and Microvascular Techniques
- Photodynamic Therapy Research Studies
- Surgical site infection prevention
- Nanoplatforms for cancer theranostics
- Tissue Engineering and Regenerative Medicine
- Immune cells in cancer
- Electrospun Nanofibers in Biomedical Applications
- Antimicrobial Peptides and Activities
- Nail Diseases and Treatments
- Trauma and Emergency Care Studies
- Diagnosis and Treatment of Venous Diseases
- Silk-based biomaterials and applications
- Zebrafish Biomedical Research Applications
- Corneal Surgery and Treatments
- Aquaculture disease management and microbiota
- Collagen: Extraction and Characterization
- Innovations in Medical Education
- Forensic Entomology and Diptera Studies
University of Wisconsin–Madison
2016-2025
University of Wisconsin Health
2024
University of Wisconsin System
2024
Crystallume (United States)
2024
Fitchburg State University
2024
Wake Forest University
2023
Louisiana State University Health Sciences Center New Orleans
2023
Loyola University Medical Center
2023
University Medical Center New Orleans
2023
Atrium Health Wake Forest Baptist
2020-2022
Skin wound healing is a major health care issue. While electric stimulations have been known for decades to be effective facilitating skin recovery, practical applications are still largely limited by the clumsy electrical systems. Here, we report an efficient bandage accelerated healing. On bandage, alternating discrete field generated wearable nanogenerator converting mechanical displacement from movements into electricity. Rat studies demonstrated rapid closure of full-thickness...
Wound repair is controlled temporally and spatially to restore tissue homeostasis. Previously we reported that thermal damage of the larval zebrafish fin disrupts collagen organization wound healing compared tail transection (LeBert et al., 2018). Here characterize different injury models in dissect temporal spatial dynamics complex damage. We found each model triggers distinct inflammatory responses, with Stat3 TGFβ playing key roles regulation mesenchymal cells during simple repair. While...
Tissue injury leads to early wound-associated reactive oxygen species (ROS) production that mediate tissue regeneration. To identify mechanisms function downstream of redox signals modulate regeneration, a vimentin reporter mesenchymal cells was generated by driving GFP from the promoter in zebrafish. Early signaling mediated activity at wound margin. Moreover, both ROS and were necessary for collagen reorganization into projections leading edge wound. Second harmonic generation time-lapse...
Polymicrobial biofilms are a hallmark of chronic wound infection. The forces governing assembly and maturation these microbial ecosystems largely unexplored but the consequences on host response clinical outcome can be significant. In context healing, formation biofilm stable community structure is associated with impaired tissue repair resulting in non-healing wound. These types wounds persist for years simmering below threshold classically defined infection (which includes heat, pain,...
Abstract Slough is a well‐known feature of non‐healing wounds. This pilot study aims to determine the proteomic and microbiologic components slough as well interrogate associations between wound healing. Ten subjects with slow‐to‐heal wounds visible were enrolled. Aetiologies included venous stasis ulcers, post‐surgical site infections pressure ulcers. Patient co‐morbidities healing outcome at 3‐months post‐sample collection was recorded. Debrided analysed microscopically, through untargeted...
Background: Large wounds often require temporary allograft placement to optimize the wound bed and prevent infection until permanent closure is feasible. We developed clinically tested a second-generation living human skin substitute (StrataGraft). StrataGraft provides both dermis fully-stratified, biologically-functional epidermis generated from pathogen-free, long-lived keratinocyte progenitor cell line, Neonatal Immortalized KeratinocyteS (NIKS). Methods: Histology, electron microscopy,...
It is unknown whether variations in burn care affect outcomes or the success of emerging therapeutics. The purpose this study was to assess surgeons' preferences excision and grafting determine if surgical technique affects outcomes. A 71-item survey evaluating skin techniques emailed members American Burn Association July August 2015. anonymous voluntary. Relationships between variables were evaluated using Fisher's exact test. P-value ≤.05 deemed statistically significant. sent 607...
This phase 3 study evaluated StrataGraft construct as a donor-site sparing alternative to autograft in patients with deep partial-thickness (DPT) burns.Patients aged ≥18 years 3-49% total body surface area (TBSA) thermal burns were enrolled. In each patient, 2 DPT areas (≤2000cm2 total) of comparable depth after excision randomized either cryopreserved or autograft. Coprimary endpoints were: the difference percent treatment site and autografted at Month (M3), proportion achieving durable...
Objectives: ABRUPT was a prospective, noninterventional, observational study of resuscitation practices at 21 burn centers. The primary goal to examine with albumin or crystalloids alone, design future prospective randomized trial. Summary Background Data: No modern has determined whether use colloids for acute resuscitation. Methods: Patients ≥18 years burns ≥ 20% total body surface area (TBSA) had hourly documentation parameters 48 hours. received either alone supplemented crystalloid...
Abstract Background Electrostimulation (ES) therapy for wound healing is limited in clinical use due to barriers such as cumbersome equipment and intermittent delivery of therapy. Methods We adapted a human skin xenograft model that can be used directly examine the nanogenerator-driven ES (NG-ES) effects on vivo—an essential translational step toward application NG-ES technique healing. Results show leads rapid closure with complete restoration normal architecture within 7 days compared more...
Candida auris proliferates and persists on the skin of patients, often leading to health care-associated infections with high mortality. Here, we describe 2 clinically relevant models show that C. grows similarly human porcine skin. Additionally, demonstrate other spp., including those phylogenetic similarity auris, do not display growth in microenvironment. These studies highlight utility ex vivo colonization allow reproducible differentiation among which should be a useful tool for...
Abstract Introduction Photodynamic therapy (PDT) is widely used to treat skin tumors and infections where protoporphyrin IX (PpIX) a commonly as the photosensitizer. PpIX synthesized through heme pathway from its precursor 5-aminolevulinic acid (5-ALA) endogenously can be induced topical application of 5-ALA. In contrast traditional PpIX-PDT, low-dose PpIX-PDT utilizes lower concentrations photosensitizer and/or irradiation doses generate level ROS, which has been shown promote burn wound...
Abstract Introduction The primary method to evaluate healing capacity of a burn wound is visual assessment; subjective interpretation that risks over-excision. This ongoing study investigates the use indocyanine green (ICG) fluorescence imaging depth and potential. Early enrollment challenges encountered with heterogeneity human wounds, difficulty patient recruitment early after trauma, variable timing assessment post-injury led addition parallel translational pre-clinical swine model....
Abstract Introduction A next-generation transparent biosynthetic wound matrix was developed for the treatment of partial-thickness burn injuries. It is composed an outer slitted 2D silicone epidermal analogue, with customizable pores, which facilitates diffusion gas and exudate from bed. The sheet bonded to a nylon knitted fabric coated porcine gelatin aloe induce adherence until healed. purpose this study compare commonly used silver-containing foam dressing burns. Methods multicenter,...
ABSTRACT Broad-spectrum antiseptics such as chlorhexidine gluconate (CHG) have widespread use pre-surgical tools to lower skin microbial burden and reduce the risk of surgical site infection. However, short- long-term effects CHG on healthy communities remain undefined due confounding binding with persistent bacterial DNA surface. Here, we aim accurately characterize immediate impact preparation CHG-based human microbiome. Twenty-eight patients undergoing elective surgeries were enrolled....
Abstract A next-generation transparent biosynthetic wound matrix (PermeaDerm) was developed for the treatment of burn injuries. Improvements include slits in an outer 2D silicone epidermal analog allowing customizable moisture control, and updated biocoating nylon knitted fabric to optimize adherence. The purpose this randomized, single-blinded, multicenter trial compare PermeaDerm a commonly used silver-containing foam dressing (Mepilex Ag) partial-thickness burns. Patients with burns...
When skin is compromised, a cascade of signals initiates the rapid repair epidermis to prevent fluid loss and provide defense against invading microbes. During this response, keratinocytes produce host peptides (HDPs) that have antimicrobial activity diverse set pathogens. Using nonviral vectors we genetically modified novel, nontumorigenic, pathogen-free human keratinocyte progenitor cell line (NIKS) express cathelicidin HDP in tissue-specific manner. NIKS tissue expresses elevated levels...
Abstract The innate immune system differentially regulates the expression of host defense peptides to combat infection during wound healing. We enhanced a peptide, human beta defensin‐3 ( hBD ‐3), in keratinocytes generate three‐dimensional biologic dressing improve healing infected wounds. NIKS keratinocyte cell line was stably transfected ex vivo with construct containing an epidermis‐specific promoter driving ‐3 ) using nonviral methods. Levels mRNA and protein skin tissue produced from...
Abstract Background Vascular surgical care has changed dramatically in recent years with little knowledge of the impact system failures on patient safety. The primary aim this multicentre observational study was to define landscape failures, errors and inefficiency (collectively termed failures) aortic surgery. Secondary aims were investigate determinants these their relationship outcomes. Methods Twenty vascular teams at ten English hospitals trained structured self-reporting intraoperative...