A5033798933- Hemoglobinopathies and Related Disorders
- Epigenetics and DNA Methylation
- Iron Metabolism and Disorders
- Prenatal Screening and Diagnostics
- Blood groups and transfusion
- Cancer-related gene regulation
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- RNA modifications and cancer
- Protease and Inhibitor Mechanisms
- Neonatal Health and Biochemistry
- Blood properties and coagulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Food Security and Health in Diverse Populations
- Platelet Disorders and Treatments
- Folate and B Vitamins Research
- DNA and Nucleic Acid Chemistry
- Erythropoietin and Anemia Treatment
- Phagocytosis and Immune Regulation
- Biological Research and Disease Studies
- Bone and Joint Diseases
- Connective tissue disorders research
- Vector-borne infectious diseases
- Cardiac Arrhythmias and Treatments
- Effects and risks of endocrine disrupting chemicals
University of Illinois Chicago
2015-2025
Jesse Brown VA Medical Center
2010-2023
Baptist Hospital of Miami
2023
University of Cauca
2015
Universitat Autònoma de Barcelona
2000-2002
Hospital Clínico Universitario de Valencia
1987-1989
Background Sickle cell disease (SCD), a congenital hemolytic anemia that exacts terrible global morbidity and mortality, is driven by polymerization of mutated sickle hemoglobin (HbS) in red blood cells (RBCs). Fetal (HbF) interferes with this polymerization, but HbF epigenetically silenced from infancy onward DNA methyltransferase 1 (DNMT1). Methods findings To pharmacologically re-induce DNMT1 inhibition, first-in-human clinical trial (NCT01685515) combined 2 small molecules—decitabine to...
Increased fetal hemoglobin levels lessen the severity of symptoms and increase lifespan patients with sickle cell disease. Hydroxyurea, only drug currently approved for treatment disease, is not effective in a large proportion therefore new pharmacological agents that have long been sought. Recent studies identifying LSD-1 as repressor γ-globin expression led to experiments demonstrating inhibitor RN-1 increased mouse model. Because arrangement developmental stage-specific pattern β-like...
Summary We describe a patient with sickle cell disease (SCD) and elevated antiphospholipid antibodies (aPL) who developed multi‐organ failure resembling catastrophic syndrome. Autoimmune screening revealed several autoantibodies characteristic of systemic lupus erythematosus (SLE). Notably, routinely housed unmanipulated transgenic mice displayed significantly titres aPL‐ SLE‐associated autoantibodies. hypothesize that SCD may be risk factor not only for the development aPL but also more...
Paratuberculosis was diagnosed in a population of approximately 1,000 free-ranging fallow deer (Dama dama) sampled from 1997–98 the Regional Hunting Reserve El Sueve (Asturias, Spain). Five eight animals observed with diarrhea were as having paratuberculosis on basis gross lesions at postmortem examination and histopathology. In two deer, Mycobacterium avium subsp. cultured identified by polymerase chain reaction. Indirect enzyme-linked immunosorbent assay immunodiffusion tests used to...
After the administration of cytostatic drugs, an increase in thromboembolic phenomena has been described cancer patients. The authors studied changes plasmatic coagulation and fibrinolysis 40 patients with nonoperable Stage III IV lung after chemotherapy. results show significant postchemotherapy increases fibrinopeptide A levels, as well a decrease fibrinolytic activity reflected by drop functional tissue activator. Also potential accumulative effect three chemotherapy cycles. plasminogen...
The mechanism responsible for developmental stage-specific regulation of γ-globin gene expression involves DNA methylation. Previous results have shown that the promoter is nearly fully demethylated during fetal liver erythroid differentiation and partially adult bone marrow differentiation. hypothesis 5-hydroxymethylcytosine (5hmC), a known intermediate in demethylation pathways, involved was investigated by analyzing levels 5-methylcytosine (5mC) 5hmC at CCGG site within 5′ region...
Background: There has been great interest in the identification of surface molecules expressed by AML cells order to selectively target tumor for destruction.One potential therapeutic is CD33, a myeloid-specific receptor that overexpressed on nearly 90% blasts.Objectives: To show CD33-positive can be specifically targeted destruction through use chimeric antigen (CAR) cytotoxic T-lymphocytes (CTL).Design/Method: We developed CAR links humanized CD33-specific single chain Fv 41BB-TCRz...
Key Points A reduction in hemolysis with voxelotor analog, GBT1118, reduced hemoglobinuria and kidney injury biomarkers transgenic sickle mice. Improved chronic preserved function histopathologic ultrastructural changes
This study was performed to investigate the hypothesis that erythroid micro-environment plays a role in regulation of globin gene expression during adult differentiation. Adult baboon bone marrow and human cord blood CD34+ progenitors were grown methylcellulose, liquid media, co-culture with stromal cell lines derived from different developmental stages identical media supporting differentiation examine effect on expression. express high levels γ-globin methylcellulose but low, physiological...
Abstract Haptoglobin (HP) is an acute-phase protein and the main scavenger of cell-free hemoglobin. When HP depleted, as observed in hemolytic conditions such sickle cell disease (SCD), hemoglobin can lead to acute organ damage. The impact 1-1, 2-1, 2-2 isoforms on concentrations SCD-related complications unclear. In a longitudinal cohort patients with SCD, 1 allele was associated higher lower at routine clinic visit well during hospitalization for vaso-occlusive episode or chest syndrome....
Following the administration of cytostatic drugs, an increase in thromboembolic phenomena has been described cancer patients. Such hemostatic alterations may be related to degree hipercoa-gulability observed following chemotherapy, comparison previous levels. In terms fibrinolytic system, however, no - clearly defined have detected. We studied changes plasmatic coagulation and fibrinolysis 40 patients with non-operable stage III IV lung (30 epidermoid ad 10 microcytic neoplasms)...