A5054630704- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Multiple Myeloma Research and Treatments
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Hemoglobinopathies and Related Disorders
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- RNA Interference and Gene Delivery
- Acute Lymphoblastic Leukemia research
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Cancer therapeutics and mechanisms
- PARP inhibition in cancer therapy
- Polyomavirus and related diseases
- Chemokine receptors and signaling
- Retinoids in leukemia and cellular processes
- Protein Degradation and Inhibitors
- Chronic Lymphocytic Leukemia Research
- Erythrocyte Function and Pathophysiology
- Liver Disease and Transplantation
University of Illinois Chicago
2016-2025
University of Pennsylvania
2023-2025
Philadelphia VA Medical Center
2023-2025
Illinois College
2007-2025
Visual Sciences (United States)
2025
University of Illinois Urbana-Champaign
2014-2024
Yale University
2024
VA Connecticut Healthcare System
2024
Rajendra Hospital
2024
Hospital of the University of Pennsylvania
2023
Allogeneic hematopoietic stem cell transplantation (HSCT) is rarely performed in adult patients with sickle disease (SCD). We utilized the chemotherapy-free, alemtuzumab/total body irradiation 300 cGy regimen sirolimus as post-transplantation immunosuppression 13 high-risk SCD between November 2011 and June 2014. Patients received matched related donor (MRD) granulocyte colony–stimulating factor–mobilized peripheral blood cells, including 2 cases that were ABO incompatible. Quality-of-life...
Several signaling pathways are activated by all-trans-retinoic acid (RA) to mediate induction of differentiation and apoptosis malignant cells. In the present study we provide evidence that p38 MAP kinase pathway is in a RA-dependent manner NB-4, acute pro-myelocytic leukemia, MCF-7, breast carcinoma, cell lines. RA treatment cells induces time- dose-dependent phosphorylation p38, such results activation its catalytic domain. not inducible variant NB-4 line, NB-4.007/6, which resistant...
Human mesenchymal stem cells (MSCs) are capable of differentiating into multiple lineages including chondrocytes, osteocytes, adipocytes, and marrow stromal cells. Using a nonhuman primate model, we evaluated MSCs as targets for gene therapy. Baboon (bMSCs) cultured from bone aspirates appeared homogeneous population spindle-shaped bMSCs were adipocytes osteocytes in vitro chondrocytes vivo. genetically modified with bicistronic vector encoding the human erythropoietin (hEPO) green...
Abstract To date, there are no efficacious translational solutions for end-stage urinary bladder dysfunction. Current surgical strategies, including diversion and augmentation enterocystoplasty (BAE), utilize autologous intestinal segments (e.g. ileum) to increase capacity protect renal function. Considered the standard of care, BAE is fraught with numerous short- long-term clinical complications. Previous trials employing tissue engineering approaches regeneration have also been unable...
We report on the screening and development of haploidentical hematopoietic stem cell transplantation (HSCT) for adult patients with clinically aggressive sickle disease (SCD) at our institution. Of 50 SCD referred HSCT between January 2014 March 2017, 20% were denied by insurance. 41 initially screened, 10% lacked an available donor, 29% had elevated donor-specific antibodies (DSAs), 34% declined to proceed HSCT. All 10 who transplanted received peripheral blood cells. The initial 2...
Epigenetic therapies, including DNA methyltransferase and histone deacetylase (HDAC) inhibitors, are increasingly being considered to treat hematological malignancies, but their effects on normal hematopoietic stem cells (HSCs) remain largely unexplored. We compared the of several HDAC valproic acid (VPA) trichostatin A (TSA), alone or in combination with 5-aza-2′-deoxycytidine (5azaD) expansion HSCs. VPA induced highest CD34+CD90+ progenitor other inhibitors sequential addition 5azaD/TSA...
Here we examined the addition of intensity-modulated total marrow irradiation (TMI) delivered using a linear accelerator to myeloablative chemotherapy conditioning regimen before allogeneic hematopoietic stem cell transplantation (HSCT). In this phase I study, enrolled 14 patients with high-risk hematologic malignancies who received escalating doses TMI at 3 Gy (n = 3), 6 9 6), and 12 2) in combination intravenous (i.v.) fludarabine 160 mg/m(2) targeted busulfan (area under curve, 4800...
DDX41 is a tumor suppressor frequently mutated in human myeloid neoplasms, but whether it affects hematopoiesis unknown. Using knockout mouse, we demonstrate that required for mouse hematopoietic stem and progenitor cell (HSPC) survival differentiation, particularly of lineage cells. Transplantation Ddx41 fetal liver adult bone marrow (BM) cells was unable to rescue mice from lethal irradiation, were also defective colony formation assays. RNA-seq analysis Lin
We characterized fluorescent bone marrow cells (YFP(+) BMCs) in the thy1-YFP mouse and determine if they promote trigeminal ganglion (TG) cell neurite growth.Excimer laser annular keratectomy was performed mice, corneas were imaged. BMCs harvested from femur tibia, expression of surface markers on YFP(+) analyzed by flow cytometry. The immunosuppressive action YFP(-)) evaluated an allogenic mixed lymphocyte reaction (MLR). Neurotrophic determined compartmental transwell cultures dissociated...
Obesity and steatotic liver disease are associated with excess morbidity mortality from cardiovascular, pulmonary, metabolic, hepatic causes. Bariatric surgery has demonstrated long-term benefits in terms of weight loss rates, but barriers to its utilization persist. To evaluate the impact bariatric on outcomes cost-effectiveness among patients obesity, focusing those cirrhosis. This economic evaluation was a retrospective cohort study including US veterans older than 18 years body mass...