A5005341501- Ovarian cancer diagnosis and treatment
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Genetics, Aging, and Longevity in Model Organisms
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Electrospun Nanofibers in Biomedical Applications
- Immune Cell Function and Interaction
- Urological Disorders and Treatments
- Atrial Fibrillation Management and Outcomes
- Cancer Cells and Metastasis
- Birth, Development, and Health
- Renal Diseases and Glomerulopathies
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- Cancer Research and Treatments
- Botulinum Toxin and Related Neurological Disorders
- Cardiovascular Effects of Exercise
- Occupational and environmental lung diseases
- Acute Myeloid Leukemia Research
- Inflammasome and immune disorders
- Protein Degradation and Inhibitors
- Ultrasound and Hyperthermia Applications
- Nicotinic Acetylcholine Receptors Study
Loyola University Chicago
2023-2025
Northwestern University
2019-2024
Brigham and Women's Hospital
2024
The University of Adelaide
2024
University of California, Los Angeles
2024
Rush University Medical Center
2009-2021
Neurology, Inc
2021
Rush University
2016
Harborview Medical Center
2011
University of Washington
2011
The high mortality rate due to ovarian cancer (OVCA) is attributed the lack of an effective early detection method. Because nonspecificity symptoms at stage, most OVCA cases are detected late stages. This makes access women with early-stage disease problematic and presents a barrier development validation tests for stage in humans. Animal models used elucidate etiologies pathogenesis that difficult study Laying hen only available animal develops spontaneously; however, detailed information...
Chorionic gonadotropin (CG) is an early embryo-derived signal that known to support the corpus luteum. An in vivo baboon model was used study direct actions of human CG (hCG) on endometrium, during periimplantation period. Endometrial gene expression analyzed using microarrays. The endometrial biopsies were taken from hCG-treated (n = 5) and control 6) animals d 10 after ovulation. Class comparison identified 61 genes whose transcript levels differed between samples (48 increased, 13...
Spina bifida (SB) patients afflicted with myelomeningocele typically possess a neurogenic urinary bladder and exhibit varying degrees of dysfunction. Although surgical intervention in the form enterocystoplasty is current standard care which to remedy bladder, it still stop-gap measure associated many complications due use bowel as source replacement tissue. Contemporary tissue engineering strategies lack ability reform smooth muscle, vasculature, promote peripheral nerve growth when using...
Long-lived proteins (LLPs) have recently emerged as vital components of intracellular structures whose function is coupled to long-term stability. Mitochondria are multifaceted organelles, and their hinges on efficient proteome renewal replacement. Here, using metabolic stable isotope labeling mice combined with mass spectrometry (MS)–based proteomic analysis, we demonstrate remarkable longevity for a subset the mitochondrial proteome. We discovered that LLPs (mt-LLPs) can persist months in...
Mutations in SOD1 cause amyotrophic lateral sclerosis (ALS) through gain-of-function effects, yet the mechanisms by which misfolded mutant (mutSOD1) protein impairs human motor neurons (MNs) remain unclear. Here, we use induced-pluripotent-stem-cell-derived MNs coupled to metabolic stable isotope labeling and mass spectrometry investigate proteome-wide degradation dynamics. We find several proteins, including ALS-causal valosin-containing (VCP), predominantly acts proteasome autophagy, that...
Abstract To date, there are no efficacious translational solutions for end-stage urinary bladder dysfunction. Current surgical strategies, including diversion and augmentation enterocystoplasty (BAE), utilize autologous intestinal segments (e.g. ileum) to increase capacity protect renal function. Considered the standard of care, BAE is fraught with numerous short- long-term clinical complications. Previous trials employing tissue engineering approaches regeneration have also been unable...
Exposure to excessive sound causes noise-induced hearing loss through complex mechanisms and represents a common unmet neurological condition. We investigate how noise insults affect the cochlea with proteomics functional assays. Quantitative reveals that exposure loud proteotoxicity. identify confirm hundreds of proteins accumulate, including cytoskeletal proteins, several nodes proteostasis network. Transcriptomic analysis subset genes encoding these also increases acutely after exposure,...
The mechanisms contributing to age-related deterioration of the female reproductive system are complex, however aberrant protein homeostasis is a major contributor. We elucidated exceptionally stable proteins, structures, and macromolecules that persist in mammalian ovaries gametes across lifespan. Ovaries exhibit localized structural cell-type-specific enrichment both follicular extrafollicular environments. Moreover, oocytes harbor panel long-lived including cytoskeletal, mitochondrial,...
The mechanisms contributing to age-related deterioration of the female reproductive system are complex, however aberrant protein homeostasis is a major contributor. We elucidated exceptionally stable proteins, structures, and macromolecules that persist in mammalian ovaries gametes across lifespan. Ovaries exhibit localized structural cell-type-specific enrichment both follicular extrafollicular environments. Moreover, oocytes harbor panel long-lived including cytoskeletal, mitochondrial,...
Using cDNA microarray analysis, we identified SGK1 (serum- and glucocorticoid-regulated kinase 1) as a gene aberrantly expressed in midsecretory endometrium of women with unexplained infertility. is serine/threonine involved primarily epithelial ion transport cell survival responses. Real-time quantitative PCR analysis larger, independent sample set timed to coincide the period uterine receptivity confirmed increased expression transcripts infertile compared fertile controls. We further...
We showed there are specific ALDH1 autoantibodies in ovarian autoimmune disease and cancer, suggesting a role for pathology. However, is little information on the expression of ALDH1. Therefore, we compared normal ovary benign malignant tumors to determine if altered cancer. Since also recent interest as cancer stem cell (CSC) marker, assessed co-expression with CSC markers order potential marker cancer.mRNA protein were human serous using quantitative Reverse-Transcriptase PCR, Western blot...
Abstract Painful diabetic neuropathy (PDN) is an intractable complication affecting 25% of patients. characterized by neuropathic pain accompanied dorsal root ganglion (DRG) nociceptor hyperexcitability, resulting in calcium overload, axonal degeneration, and loss cutaneous innervation. The molecular pathways underlying these effects are unknown. Using high-throughput deep-proteome profiling, we found that mitochondrial fission proteins were elevated DRG neurons from mice with PDN induced a...
Background: Understanding the mechanisms of cardiomyocyte development is critical for fulfilling potential induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Although myocyte known to depend on internal and external mechanical cues, further investigation required understand contributions different signals how they are integrated together generate an adult cardiomyocyte. Here, we address this gap by examining role calcium-activated contractility in sarcomere formation maturation...
Atrial fibrillation (AF) is the most common arrhythmia, with few treatment options. To discover novel pathways, we performed mass spectrometry (MS) on atrial tissue from patients in Sinus Rhythm or AF without heart failure. We identified changes canonical although surprisingly, contractile proteins and specifically a loss of isoforms. Functional remodeling was confirmed cardiomyocytes, revealing increased contractility compared to SR. MS analysis human ventricular found that ~1/3 proteomic...
<h3>Objective</h3> Tumor-associated neoangiogenesis (TAN) is an early event in ovarian tumor development. Interleukin 16 (IL-16) a proangiogenic cytokine that stimulates production of neoangiogenic factors. The goal this study was to determine the association IL-16 with development and TAN laying hens, animal model spontaneous cancer (OVCA). <h3>Methods</h3> Sera tissues from 3-year-old hens were collected processed for histopathologic, immunoassay, immunohistochemistry, immunoblotting,...
Chronic inflammation fundamentally influences cancer risk and development. A mechanism of chronic is the formation inflammasome complexes which results in sustained secretion pro-inflammatory cytokines IL1β IL18. Inflammasome expression actions vary among cancers. There no information on ovarian (OvCa). To determine if tumors express components, mRNA protein NLRP3 (nucleotide-binding domain, leucine-rich repeat family, pyrin domain containing 3), caspase-1, IL1β, IL18 hen human OvCa was...
Atrial fibrillation (AFib) is the most common cardiac rhythm disturbance, often treated via electrical cardioversion. Following restoration, a period of depressed mechanical function known as atrial stunning occurs, suggesting that defects in contractility occur AFib and are revealed upon restoration rhythm. This project aims to define contractile remodeling occurs AFib. To assess function, we used canine tachypacing model induced Mass spectrometry analysis showed dysregulation proteins...
The lenticular fiber cells are comprised of extremely long-lived proteins while still maintaining an active biochemical state. Dysregulation these activities has been implicated in diseases such as age-related cataracts. However, the protein dynamics underlying health and disease is unclear. We sought to measure global turnover rates eye using nitrogen-15 labeling mice mass spectrometry. measured 14N/15N-peptide ratios 248 lens proteins, including Crystallin, Aquaporin, Collagen enzymes that...
Objective Animal models of spontaneous ovarian cancer are important for understanding early tumor development. Ovarian imaging may play an role in following changes Laying hens the only animals that develop similar to humans. The aim this study was determine feasibility detecting tumors laying using sonography. Methods Ovaries commercial strains White Leghorn (n = 29, 2.5–3.0 years old) were examined by transvaginal 2-dimensional gray scale and color Doppler Sonographic evaluations compared...