A5019773715- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Curcumin's Biomedical Applications
- Porphyrin Metabolism and Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Drug Transport and Resistance Mechanisms
- Biochemical and Molecular Research
- Drug-Induced Hepatotoxicity and Protection
- SARS-CoV-2 and COVID-19 Research
- Pharmacological Effects of Medicinal Plants
- Synthesis and bioactivity of alkaloids
- HIV/AIDS drug development and treatment
- Vibrio bacteria research studies
- Neonatal Health and Biochemistry
- Traditional and Medicinal Uses of Annonaceae
- Invertebrate Immune Response Mechanisms
- Trypanosoma species research and implications
- Natural product bioactivities and synthesis
- Insect symbiosis and bacterial influences
- Pharmacological Effects and Toxicity Studies
- Complement system in diseases
- Insect Pest Control Strategies
- Porphyrin and Phthalocyanine Chemistry
- Essential Oils and Antimicrobial Activity
Institute of Life Sciences
2017-2025
Mie University
2022
Indian Institute of Science Bangalore
2006-2015
Artemisinin and curcumin show an additive interaction in killing Plasmodium falciparum culture. In vivo, 3 oral doses of following a single injection alpha,beta-arteether to berghei-infected mice are able prevent recrudescence due monotherapy ensure almost 100% survival the animals.
Heme metabolism is central to malaria parasite biology. The acquires heme from host hemoglobin in the intraerythrocytic stages and stores it as hemozoin prevent free toxicity. can also synthesize de novo, all enzymes pathway are characterized. To study role of dual sources growth development, we knocked out first enzyme, δ-aminolevulinate synthase (ALAS), last ferrochelatase (FC), heme-biosynthetic Plasmodium berghei (Pb). wild-type knockout (KO) parasites had similar patterns mice. We...
Abstract Curcumin has many pharmacological activities despite its poor bioavailability and in vivo stability. Here, we show that a nanoformulated curcumin (PLGA-curcumin) better therapeutic index than native preventing the onset of neurological symptoms delaying death mice experimental cerebral malaria. Oral PLGA-curcumin was at least as effective 15-fold lower concentration breakdown blood-brain barrier inhibition brain mRNAs for inflammatory cytokines, chemokine receptor CXCR3 ligand...
Earlier studies in this laboratory have shown the potential of artemisinin-curcumin combination therapy experimental malaria. In a parasite recrudescence model mice infected with Plasmodium berghei (ANKA), single dose alpha,beta-arteether (ART) three oral doses curcumin prevented recrudescence, providing almost 95% protection. The parasites were completely cleared blood ART-alone (AE) or ART+curcumin (AC) treatments short-term, although clearance was faster latter case involving increased...
Coronavirus disease 2019 (COVID-19) is caused by a Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2), which positive-strand RNA virus. The SARS-CoV-2 genome and its association to SAR-CoV-1 vary from ca. 66 96% depending on the type of betacoronavirideae family members. With several drugs, viz. chloroquine, hydroxychloroquine, ivermectin, artemisinin, remdesivir, azithromycin considered for clinical trials, there has been an inherent need find distinctive antiviral mechanisms...
Heme-biosynthetic pathway of malaria parasite is dispensable for asexual stages, but essential mosquito and liver stages. Despite having backup mechanisms to acquire hemoglobin-heme, intermediates and/or enzymes from the host, parasites express heme synthesize heme. Here we show synthesized in stages promotes cerebral pathogenesis by enhancing hemozoin formation. Hemozoin a molecule associated with inflammation, aberrant host-immune responses, disease severity pathogenesis. The knockout less...
An organized regulation of gene expression and DNA replication is vital for the progression complex life cycle P. falciparum (Pf), involving multiple hosts various stages. These attributes rely on dynamic architecture chromatin governed by several factors, including histone chaperones. Nucleoplasmin class chaperones perform chaperoning function participate in developmental processes eukaryotes. Here, our crystal structure confirmed that Pf indeed possesses a nucleoplasmin isoform (PfNPM),...
The hybrid pathway for heme biosynthesis in the malarial parasite proposes involvement of genome-coded enzymes localized different compartments such as apicoplast, mitochondria, and cytosol. However, knowledge on functionality localization many these is not available. In this study, we demonstrate that porphobilinogen deaminase encoded by Plasmodium falciparum genome (PfPBGD) has several unique biochemical properties. Studies carried out with PfPBGD partially purified from membrane fraction,...
ADVERTISEMENT RETURN TO ISSUEPREVLetter to the EditorNEXTCurcumin May Defy Medicinal ChemistsGovindarajan Padmanaban*† and Viswanathan A. Nagaraj‡View Author Information† Department of Biochemistry, Indian Institute Science, Bangalore-560012, India‡ Infectious Disease Biology, Life Sciences, Bhubaneswar-751023, India*E-mail: [email protected]. Phone: +91 80 23601492.Cite this: ACS Med. Chem. Lett. 2017, 8, 3, 274Publication Date (Web):February 15, 2017Publication History Received6 February...
Abstract The proteins of Plasmodium , the malaria parasite, are strikingly rich in asparagine. depends primarily on host haemoglobin degradation for amino acids and has a rudimentary pathway acid biosynthesis, but retains gene encoding asparagine synthetase (AS). Here we show that deletion AS berghei ( Pb ) delays asexual- liver-stage development with substantial reduction formation ookinetes, oocysts sporozoites mosquitoes. In absence synthesis, extracellular supports suboptimal survival...
Corona virus disease 2019 (COVID-19) is caused by a Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2), which positive strand RNA virus. The SARS-CoV-2 genome and its association to SAR-CoV-1 vary from ca. 66% 96% depending on the type of betacoronavirdeae family members. With several drugs, viz. chloroquine, hydroxychloroquine, ivermectin, artemisinin, remdesivir, azithromycin considered for clinical trials, there has been an inherent need find distinctive antiviral mechanisms...
Abstract Malaria parasite lacks canonical pathways for amino acid biosynthesis and depends primarily on hemoglobin degradation extracellular resources acids. Interestingly, a putative gene glutamine synthetase (GS) is retained despite being an abundant in human mosquito hosts. Here we show Plasmodium GS has evolved as unique type I enzyme with distinct structural regulatory properties to adapt the asexual niche. Methionine sulfoximine (MSO) phosphinothricin (PPT) inhibit activity. localized...
Corona virus disease 2019 (COVID-19) is caused by a Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2), which positive strand RNA virus. The SARS-CoV-2 genome and its association to SAR-CoV-1 vary from ca. 66% 96% depending on the type of betacoronavirdeae family members. With several drugs, viz. chloroquine, hydroxychloroquine, ivermectin, artemisinin, remdesivir, azithromycin considered for clinical trials, there has been an inherent need find distinctive antiviral mechanisms...