A5017478122- Pregnancy and preeclampsia studies
- CAR-T cell therapy research
- Birth, Development, and Health
- COVID-19 Impact on Reproduction
- Cardiovascular Issues in Pregnancy
- Hematopoietic Stem Cell Transplantation
- Pregnancy and Medication Impact
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Single-cell and spatial transcriptomics
- Vasculitis and related conditions
- Otitis Media and Relapsing Polychondritis
- Kruppel-like factors research
- Extracellular vesicles in disease
- Nutrition and Health in Aging
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Mechanical Circulatory Support Devices
- Cardiac Arrhythmias and Treatments
- Gestational Diabetes Research and Management
- Biosimilars and Bioanalytical Methods
- Dialysis and Renal Disease Management
- Biochemical effects in animals
- Antiplatelet Therapy and Cardiovascular Diseases
- Biomedical Ethics and Regulation
- Eosinophilic Disorders and Syndromes
- Platelet Disorders and Treatments
Intarcia Therapeutics (United States)
2023-2025
Universitat de Barcelona
2020-2025
Hospital Clínic de Barcelona
2021-2025
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2020-2025
Josep Carreras Leukaemia Research Institute
2021-2023
Fundación Josep Carreras Contra la Leucemia
2023
COVID-19 presents a spectrum of signs and symptoms in pregnant women that might resemble preeclampsia. Differentiation between severe preeclampsia is difficult some cases.To study biomarkers endothelial damage, coagulation, innate immune response, angiogenesis pregnancy addition to vitro alterations cells exposed sera from with COVID-19.Plasma samples were obtained infection classified into mild (n=10) or (n=9) normotensive pregnancies as controls patients (n=13). A panel plasmatic was...
JAK2V617F-mutated myeloproliferative neoplasms (MPN) exhibit abnormal proliferation of bone marrow progenitors and increased risk thrombosis, specifically in splanchnic veins (SVT). The contribution the endothelium to development prothrombotic phenotype was explored. Plasma serum samples from JAK2V617F MPN patients with (n=26) or without (n=7) thrombotic debut different treatments, were obtained (n=33). Cultured endothelial cells (ECs) exposed these healthy donors as controls. Changes...
Chimeric antigen receptor (CAR)-T cell-based immunotherapy constitutes a revolutionary advance for treatment of relapsed/refractory hematological malignancies. Nevertheless, cytokine release and immune effector cell-associated neurotoxicity syndromes are life-threatening toxicities in which the endothelium could be pathophysiological substrate. Furthermore, differential diagnosis from sepsis, highly incident these patients, is challenging. Suitable laboratory tools determinant their...
Abstract Background The elderly and immunocompromised remain at high risk of severe COVID-19 due to suboptimal immune responses. Monoclonal antibodies SARS-CoV-2 spike were highly effective for both prophylaxis treatment, but quickly became ineffective viral escape. Using a machine learning-guided optimization approach, we identified two against conserved epitopes, one targeting the S2 stem helix (GB-0669) other class 4 RBD site on S1 (PRO-37587) with goal developing combination that would...
Abstract Introduction Our objective was to evaluate the endothelial function profile and complement system in fetuses from preeclamptic pregnancies using ex vivo vitro approaches. Material Methods A total of 66 singleton were prospectively recruited comprising 34 cases preeclampsia 32 normotensive matched for baseline characteristics. In approach, soluble tumor necrosis factor‐a receptor 1 (sTNFR1), vascular cell adhesion molecule‐1 (sVCAM‐1), intercellular (sICAM‐1), Von Willebrand factor...
Preeclampsia (PE) is a pregnancy complication characterized by hypertension, proteinuria, endothelial dysfunction, and complement dysregulation. Placenta-derived extracellular vesicles (EVs), necessary in maternal-fetal communication, might contribute to PE pathogenesis. Moreover, neutrophil traps (NETs) play pathogenic role other complement-mediated pathologies, their contribution remains unexplored.
Identification of qualitative variants von Willebrand disease (VWD) can be a diagnostic challenge because discrepant results obtained in the multiple laboratory tests available for its appropriate classification. We report two cases infrequent inherited VWD with unclear preliminary test panel at time first consultation and that were finally diagnosed as type 2A/IID c.8318 G > C, p.Cys2773Ser mutation 2M c.4225 T G, p.Val1409Phe mutation, respectively. The description these highlights despite...
Abstract Background The clinical benefit of anti-spike mAbs for prophylaxis and treatment SARS-CoV-2 is now well established (Baum 2020, Loo 2022, Westendorf Jones 2021, Kreuzberger Cathcart Hirsch Shi 2020); However, the evolution SARS CoV-2 due to immune pressure has compromised utility all previously approved which target variable, dominant receptor binding domain Spike protein. Despite success vaccination protecting general population, immunocompromised elderly remain at high risk...
To investigate the contribution of placenta-derived extracellular vesicles (pdEVs) in pre-eclampsia (PE) associated endothelial dysfunction. Sera samples from 8 PE and 6 normotensive pregnancies were pooled 3 pools 1 control pool. PdEVs isolated by differential centrifugation. assess damage, cells culture incubated (48h) with or sera presence absence eVs (depleted sera). Then, changes VCAM-1, ICAM-1, VWF expression ROS production assessed. The results expressed as average fold increase...
Background: Extracellular traps (ETs) formed by neutrophils (NETs) and eosinophils (EETs) have been described in coronary thrombi, contributing to thrombus stability.A key mechanism during NET formation is histone modification the enzyme PAD4.Citrullinated histones, product of PAD4 activity, are often attributed neutrophils.Eosinophils also express high levels but contribution EET not known.Aims: To characterize role relevance thrombosis.Methods: We performed immunohistological analyses on...
To study endothelial damage, microvascular thrombosis, immune response and angiogenic imbalance in pre-eclampsia (PE) COVID-19 pregnancy. Plasma sera samples were obtained from pregnant women with infection classified into mild (n=10) or severe (n=9) addition to normotensive pregnancies as controls patients PE (n=13). Biomarker assessment included circulating VCAM-1, TNF-receptor I (TNFRI), angiotensin II (ANGII), heparan sulfate (HS), thrombomodulin (TM), VWF antigen, activity multimeric...
To explore the performance of endotheliopathy biomarkers and angiogenic factors in distinguishing pre-eclampsia (PE) from COVID-19 pregnancy. Plasma sera samples were obtained pregnant women with infection (n = 18) patients PE 13). Biomarker assessment included circulating VCAM-1, TNF-receptor I (TNFRI), angiotensin II (ANGII), heparan sulfate (HS), thrombomodulin (TM), C5b9, PAI-1, ADAMTS-13 activity, fms-like tyrosine kinase-1 (sFlt1) placental growth factor (PlGF). The area under ROC...
To study endothelial damage and alteration in signalling pathways cells exposed to sera from pregnant women with PE COVID-19 vitro. Sera samples were obtained infection classified into mild (n=10) or severe (n=9) addition normotensive pregnancies as controls patients (n=13). Endothelial the human dermal microvascular cell line. Nuclei deposits of ICAM-1and Von Willebrand factor (VWF) staining was performed, micrographs captured by fluorescent microscopy. The area covered ICAM-1 VWF labelling...