A5065417198- Cell death mechanisms and regulation
- Microbial metabolism and enzyme function
- Advancements in Transdermal Drug Delivery
- Biological Activity of Diterpenoids and Biflavonoids
- Metal-Catalyzed Oxygenation Mechanisms
- Dye analysis and toxicity
- Essential Oils and Antimicrobial Activity
- Free Radicals and Antioxidants
- Redox biology and oxidative stress
- Synthesis and Biological Evaluation
- Biochemical Acid Research Studies
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Genomics, phytochemicals, and oxidative stress
- Metal complexes synthesis and properties
- RNA Interference and Gene Delivery
- Dermatology and Skin Diseases
- RNA and protein synthesis mechanisms
- Glutathione Transferases and Polymorphisms
- Effects and risks of endocrine disrupting chemicals
- Endoplasmic Reticulum Stress and Disease
- Bioactive Compounds and Antitumor Agents
- Autophagy in Disease and Therapy
- Phytochemistry Medicinal Plant Applications
- Inflammatory mediators and NSAID effects
Universidade Lusófona
2016-2025
Technologies pour la Santé
2014-2024
University of Cambridge
2012-2019
Rede de Química e Tecnologia
2017
Imperial College London
2012-2013
University of Lisbon
2011
Poor drug solubility represents a problem for the development of topical formulations. Since ionic liquids (ILs) can be placed in either lipophilic or hydrophilic solutions, they may advantageous vehicles such delivery systems. Nonetheless, it is vital to determine their usefulness when used at concentrations were cell viability maintained, which was considered herein.Five different ILs prepared-three imidazole-based ILs: [C2mim][Br], [C4mim][Br], and [C6mim][Br]; two choline-based...
Golgi antiapoptotic proteins (GAAPs) are highly conserved membrane that inhibit apoptosis and promote Ca(2+) release from intracellular stores. Given the role of in controlling cell adhesion motility, we hypothesized human GAAP (hGAAP) might influence these events. In this paper, present evidence hGAAP increased adhesion, spreading, migration a manner depended on C-terminal domain hGAAP. We show store-operated entry thereby activity calpain at newly forming protrusions. These hGAAP-dependent...
Manganese(III) porphyrins (MnPs) are superoxide dismutase (SOD) mimics with demonstrated beneficial effects in cancer treatment combination chemo- and radiotherapy regimens. Despite the ongoing clinical trials, little is known about effect of MnPs on metastasis, being therefore essential to understand how affect this process. In present work, impact MnP MnTnHex-2-PyP5+ metastasis-related processes was assessed breast cells (MCF-7 MDA-MB-231), alone or doxorubicin (dox). The co-treatment...
Golgi anti-apoptotic proteins (GAAPs) are multitransmembrane that expressed in the apparatus and able to homo-oligomerize. They highly conserved throughout eukaryotes present some prokaryotes orthopoxviruses. Within eukaryotes, GAAPs regulate Ca(2+) content of intracellular stores, inhibit apoptosis, promote cell adhesion migration. Data presented here demonstrate purified viral (vGAAPs) human Bax inhibitor 1 form ion channels vGAAP from camelpox virus is selective for cations. Mutagenesis...
Elevated expression levels of the apurinic/apyrimidinic endonuclease 1 (APE1) have been correlated with more aggressive phenotypes and poor prognosis non-small cell lung cancer (NSCLC). This study aimed to assess impact inhibition redox function APE1 E3330 either alone or in combination cisplatin NSCLC cells. For this purpose, complementary endpoints focusing on viability, apoptosis, cycle distribution, migration/invasion were studied. Cisplatin decreased viability H1975 cells a time-...
LOX (Lysyl oxidase) and like 1-4 (LOXL1-4) are amine oxidases that catalyse the cross-linking of elastin collagen in extracellular matrix (ECM). This activity can facilitate cell migration formation metastases. Consequently, inhibition these enzymes and, particular LOXL2, has been suggested as a therapeutic strategy to prevent breast cancer metastasis. Although medicinal chemistry studies have struggled specifically inhibit importance selectivity this context is not clear. To explore role...
Manganese(III) porphyrin MnTnBuOE-2-PyP5+ (MnBuOE, BMX-001) is a third-generation redox-active cationic substituted pyridylporphyrin-based drug with good safety/toxicity profile that has been studied in several types of cancer. It currently four phase I/II clinical trials on patients suffering from glioma, head and neck cancer, anal squamous cell carcinoma multiple brain metastases. There yet an insufficient understanding the impact MnBuOE lung Therefore, this study aims to fill gap by...
Golgi anti-apoptotic proteins (GAAPs) are hydrophobic resident in membranes of the complex. They protect cells from a range apoptotic stimuli, reduce Ca(2+) content intracellular stores, and regulate fluxes. GAAP was discovered camelpox virus, but it is highly conserved throughout evolution encoded by all eukaryote genomes examined. GAAPs part transmembrane Bax inhibitor-containing motif (TMBIM) family that also includes other Ca(2+)-modulating membrane proteins. Most TMBIM members show...
The human apurinic/apyrimidinic endonuclease 1 (APE1) is an ubiquitous multifunctional DNA repair enzyme and a redox signalling protein. Our work addressed the inhibition of APE1 function using E3330, as single agent or in combination with docetaxel (DTX), breast cancer MDA‐MB‐231 cells. E3330 decreased colony formation DTX‐treated In addition, alone significantly reduced collective cell migration assessed by wound‐healing assay, whereas combined treatment chemoinvasion. These results...
Multiple mechanisms related to metastases undergo redox regulation. Cu[15]pyN5 is a redox-active copper(II) complex previously studied as chemotherapy sensitizer in mammary cells. The effects of cotreatment with and doxorubicin (dox) were evaluated two human breast cancer cell lines: MCF7 (low aggressiveness) MDA-MB-231 (highly aggressive). decreased MCF7-directed migration. In addition, dox reduced the proteolytic invasion Cell detachment was not affected by exposure these agents....
Clear-cell renal carcinoma (ccRCC) is the most common type of cancer. The importance oxidative stress in context this disease has been described, although there only little information concerning role superoxide dismutase (SOD) enzymes. SOD different pathological conditions promoted development mimics (SODm). As such, manganese(III) porphyrins can mimic natural enzymes and scavenge reactive oxygen species (ROS), thus modulating cellular redox status. In study, exposure 786-O human cancer...
Transfersomes have been highlighted as an interesting nanotechnology-based approach to facilitate the skin delivery of bioactive compounds. Nevertheless, properties these nanosystems still need be improved enable knowledge transfer pharmaceutical industry and development more efficacious topical medicines. Quality-by-design strategies, such Box-Behnken factorial design (BBD), are in line with current use sustainable processes develop new formulations. Thus, this work aimed at optimizing...
Parvifloron D is a natural diterpene with broad and not selective cytotoxicity toward human tumor cells. In order to develop targeted antimelanoma drug delivery platform for D, hybrid nanoparticles were prepared biopolymers functionalized α-melanocyte stimulating hormone. Results/methodology: Nanoparticles produced according solvent displacement method the physicochemical properties assessed. It was shown that cytotoxic can induce, both as free encapsulated drug, cell death in melanoma cells...
Medicinal plants of the Plectranthus genus (Lamiaceae) are known for their ethnopharmacological relevance, mainly against infectious, dermatologic and gastrointestinal pathologies. Three species originated from South Africa, namely P. madagascariensis, neochilus rare porcatus were hereby screened antimicrobial cytotoxic activities related with and/or potential ethnomedicinal uses. Twenty-six extracts prepared by combination extraction methods (infusion, decoction, microwave-assisted,...
The mechanisms by which the Golgi apparatus (GA) impacts on cell invasion are poorly understood. human Anti-Apoptotic Protein (hGAAP, also known as TMBIM4) is a highly conserved cation channel that modulates intracellular Ca2+ fluxes. Human GAAP expressed in all tissues, essential for viability and provides resistance against range of apoptotic stresses. Furthermore, hGAAP enhances adhesion migration increasing turnover focal adhesions due to activation store-operated entry. Here, we...
The manganese(III) porphyrin MnTnHex-2-PyP5+ (MnTnHex) is a potent superoxide dismutase mimic and modulator of redox-based transcriptional activity that has been studied in the context different human disease models, including cancer. Nevertheless, for lung cancer, hardly any information available. Thus, present work aims to fill this gap reports effects MnTnHex non-small cell cancer (NSCLC) cells, more specifically, A549 H1975 vitro. Both lines were initially characterized terms innate...