A5004143329- Blood groups and transfusion
- Platelet Disorders and Treatments
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Blood disorders and treatments
- Lipid metabolism and disorders
- Antiplatelet Therapy and Cardiovascular Diseases
- Hemoglobinopathies and Related Disorders
- Venous Thromboembolism Diagnosis and Management
- Complement system in diseases
- Blood Coagulation and Thrombosis Mechanisms
- Chronic Lymphocytic Leukemia Research
- Blood transfusion and management
- Neonatal Health and Biochemistry
- Parasites and Host Interactions
- Renal Diseases and Glomerulopathies
- Cardiovascular Syncope and Autonomic Disorders
- Gene Regulatory Network Analysis
- Forensic Toxicology and Drug Analysis
- Research on Leishmaniasis Studies
- Parvovirus B19 Infection Studies
- Inflammatory Biomarkers in Disease Prognosis
- Glycogen Storage Diseases and Myoclonus
- Nitric Oxide and Endothelin Effects
- Delphi Technique in Research
Sanquin
2011-2025
Leiden University Medical Center
2012
University of Amsterdam
1997-2011
Amsterdam UMC Location University of Amsterdam
1998-2001
Wilhelmina Children's Hospital
2000
Dutch Blood Transfusion Society
1997-1998
The Netherlands Cancer Institute
1997
Congenital amegakaryocytic thrombocytopenia (CAMT) is a rare disorder of undefined aetiology. The disease presents with severe and absence megakaryocytes in the bone marrow. Furthermore, CAMT patients may develop marrow aplasia. To obtain more insight into mechanism underlying CAMT, five children were analysed. All had increased plasma thrombopoietin (Tpo) levels, indicating platelet production defect. Bone marrow-derived CD34+ stem cells from three cultured an vitro liquid culture system to...
Summary It has been reported that blood trombopoietin (TPO) levels can discriminate between thrombocytopenia due to increased platelet destruction and decreased production. With our TPO ELISA a glycocalicin we analysed large group of patients in detail could confirm amplify the above notion detail. were determined plasma from 178 clinically serologically well-defined thrombocytopenic patients: 72 with idiopathic autoimmune (AITP), 29 secondary AITP, 5 amegakaryocytic who suffered various...
Febrile conditions are often associated with increased platelet turnover and refractoriness to transfusions, although several pyrogenic cytokines enhance thrombopoiesis. This study aimed characterize the effects of experimental human endotoxemia on thrombopoiesis.Endotoxin (4 ng/kg) was infused into 30 healthy men regulation thrombopoiesis in systemic inflammation. Platelet counts, plasma thrombopoietin (TPO) glycocalicin levels, reticulated platelets (RP) were measured evaluate effect acute...
Thrombopoietin is produced at a constant rate by the liver and kidney removed from circulation upon binding subsequent uptake via Tpo receptor, c-Mpl, expressed platelets mega-karyocytes. Apart uptake, this study shows that can also function as storage pool for Tpo. Upon stimulation with various platelet agonists, full-length biologically active was released platelets. Platelet fractionation experiments indicated most likely contained in granules. When were preincubated Tpo-peptide mimetic...
For the rapid and simple diagnosis of schistosomiasis, a reagent strip assay for detection circulating cathodic antigen (CCA) in urine was developed. The test based on previously developed sandwich enzyme-linked immunosorbent (ELISA) using combination two anti-CCA monoclonal antibodies. assay, as capture matrix, antibody coated onto nitrocellulose membrane mounted polyvinyl chloride strips. Urine samples were then tested an consisting combined incubation step sample biotinylated detecting...
Summary In this report a sensitive enzyme-linked immunosorbent assay (ELISA) for the measurement of plasma thrombopoietin (Tpo) is described that solely based on monoclonal antibodies (MoAbs). The has an intra and inter-assay variance 5-7% 7-13%, respectively. Native recombinant human Tpo (rhTpo) were recognized equally well, no cross reactivity with other cytokines was found rhTpo added to serum completely recovered. With ELISA, concentrations in EDTA-anticoagulated all controls (n = 193)...
In autoimmune hemolytic anemia autoantibodies against erythrocytes lead to increased clearance of the erythrocytes, which in turn results a potentially fatal anemia. Depending on whether IgG or IgM antibodies are involved, response therapy is different. Proper identification isotype anti-erythrocyte is, therefore, crucial. However, detection can be challenging. We, set out improve IgM. Direct using flow cytometry-based approach did not yield satisfactory improvements. Next, we analyzed...
BACKGROUND Pregnant women are routinely screened for red blood cell (RBC) antibodies early in pregnancy. If RBC‐alloantibodies detected, repeated laboratory testing is advised to timely identify pregnancies at high risk severe hemolytic disease of the fetus and newborn (HDFN). We assessed RBC alloantibodies, other than anti‐D or anti‐K, cut‐offs titer antibody dependent cellular cytotoxicity (ADCC) test select high‐risk cases. To advise on repeat intervals, avoid unnecessary testing, we...
Abstract Background Autoimmune hemolytic anemia (AIHA) is typically mediated by immunoglobulin G (IgG) or M (IgM) antibodies, and more rarely A (IgA). The mechanism of red blood cell (RBC) destruction in IgA‐mediated AIHA not well understood. We report a case severe with intravascular hemolysis, positive for IgA. Hemolysis did subside despite multiple transfusions treatment lines, leading to fatal outcome. Here, we set out investigate underlying pathophysiological mechanisms. Study Design...
Thrombopoietin (Tpo), the main regulator of thrombocytopoiesis, is a probable candidate to play role in increase platelet counts that frequently seen after surgery. In current study, serial blood samples patients underwent major surgery were analysed with respect Tpo kinetics, turnover and inflammatory cytokines. Platelet content plasma levels rose before increased, suggesting was indeed responsible for elevation counts. addition, an interleukin 6 (IL‐6) levels, but not IL‐11 tumour necrosis...
BACKGROUND The Transfusion Register of Irregular Antibodies and Cross‐match Problems (TRIX) is a unique national database in the Netherlands that was launched 2007. laboratories register presence irregular RBC alloantibodies for their patients can consult information relevant pretransfusion testing, unknown own laboratory system. STUDY DESIGN AND METHODS Data from TRIX 10 years after implementation have been analyzed to demonstrate added value transfusion practice. antibody registration,...
Fourteen children with congenital thrombocytopenia were analysed in order to unravel the mechanisms underlying their and evaluate value of new laboratory tests, namely measurement plasma thrombopoietin (Tpo) glycocalicin (GC) levels analysis megakaryocytopoiesis vitro. Three groups patients included. The first group (n = 6) was diagnosed amegakaryocytic thrombocytopenia. They had no megakaryocytes bone marrow, three out four showed megakaryocyte formation vitro, all high Tpo low GC levels....
Background Neonates with R hesus c ( Rh ) hemolytic disease of the fetus and newborn HDFN are often managed in same way as neonates D , although evidence to support this policy is limited. The objective study was evaluate neonatal outcome severe compared . Study Design Methods A retrospective (near‐)term (n = 22) 103; without additional antibodies) admitted L eiden U niversity M edical C enter between J anuary 2000 O ctober 2011 conducted. need for intrauterine transfusions IUTs ),...
Abstract Background During pregnancy, maternal red blood cell (RBC) antibodies can lead to life‐threatening fetal hemolysis and anemia. Women become immunized by a pregnancy or an unmatched transfusion. Our aim was quantify the effect of nationwide K‐matched transfusion policy for women childbearing age potential prevent K‐immunization in pregnancy. Study Design Methods In this nation‐wide change evaluation study we determined occurrence RBC before after introduction evaluated cause K...
Abstract Complement-mediated (CM) autoimmune hemolytic anemia (AIHA) is characterized by the destruction of red blood cells (RBCs) autoantibodies that activate classical complement pathway. These antibodies also reduce transfusion efficacy via lysis donor RBCs. Because C1-inhibitor (C1-INH) an endogenous regulator pathway, we hypothesized peritransfusional C1-INH in patients with severe CM-AIHA reduces activation and hemolysis, thus enhances RBC efficacy. We conducted a prospective,...
We have previously described the private or family platelet antigen, Gro a , which was identified in case of neonatal alloimmune thrombocytopenia. The antigen found to be located on GP IIIa (β 3 ) subunit IIb/IIIa complex, most prominent fibrinogen receptor platelets. Initial experiments characterize at molecular genetic level were unsuccessful. therefore decided use different strategy unravel basis this antigen. Platelet mRNA Gro(a + and − donor amplified with suitable primers reverse...
To characterize fetal thrombopoiesis, we determined plasma thrombopoietin (TPO) and glycocalicin levels, platelet counts reticulated platelets (RP) of fetuses compared them with the respective values their mothers. Percutaneous umbilical vein sampling in abnormal pregnancies revealed twofold higher levels 20‐fold counts, but lower mothers ( P < 0·05). Neither expression glycoprotein Ib IIb on nor were different between fetuses. These data indicate enhanced thrombopoiesis and/or increased turnover
Abstract Background and Objectives: Glycocalicin (GC) is a proteolytic fragment of Gplb can conveniently be measured in supernatants platelet concentrates (PCs) by means sandwich ELISA. Because the convenience assay easy sample storage, we tested its suitability as sensitive activation parameter during PC storage. Material Methods : Filtered PCs plasma or additive solution were made from 5 pooled buffy coats subsequently stored 8 days at 22±2°C. Correlation coefficients (r) calculated after...
Thrombocytopenia with absent radii (TAR) syndrome is a rare congenital defect severe hypomegakaryocytic thrombocytopenia and bilateral radial aplasia. To elucidate possible relationship between in TAR defects the thrombopoietin (TPO)/c-Mpl system, we examined TPO activity sera from six patients vitro reactivity of patients' platelets to recombinant human TPO. We found elevated serum levels all patients, excluding production as pathomechanism for thrombocytopenia. In contrast healthy...