A5014743881- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Immune cells in cancer
- DNA Repair Mechanisms
- Renal and related cancers
- Biochemical and Molecular Research
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Adenosine and Purinergic Signaling
- Cytomegalovirus and herpesvirus research
- Genomic variations and chromosomal abnormalities
- Reproductive Biology and Fertility
- Congenital gastrointestinal and neural anomalies
- Genetic and rare skin diseases.
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Platelet Disorders and Treatments
- Renal Diseases and Glomerulopathies
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Infant Nutrition and Health
- Tumors and Oncological Cases
- Viral-associated cancers and disorders
Shanghai Children's Medical Center
2017-2025
Shanghai Jiao Tong University
2017-2025
Hirschsprung's disease-associated enterocolitis (HAEC) is the most common complication of disease (HSCR). The microbiome pattern intestinal flora in HAEC patients was significantly abnormal compared to that HSCR patients. overabundance V. parvula detected gut To elucidate pathological mechanisms parvula, we established and analyzed inflammatory models induced by LPS or single-bacterial strain transplantation vivo. response colon mice. Besides, found from can impair barrier function colonic...
Ovarian cancer is a fatal gynecologic malignancy that found worldwide and exhibits an insidious onset lack of early warning symptoms. Despite ongoing studies, the mechanistic basis aggressive phenotypes ovarian remains unclear. Lysine acetyltransferase 6A (KAT6A) MYST-type histone (HAT) enzyme identified as oncogene in breast cancer, glioblastoma leukemia. However, specific functions KAT6A remain
Most clinical PARP inhibitors (PARPis) trap PARP1 in a chromatin-bound state, leading to PARPi-mediated cytotoxicity. PARPi resistance impedes the treatment of ovarian cancer practice. However, mechanism by which cells overcome trapping develop remains unclear. Here, it is shown that high levels KAT6A promote cancer, regardless its catalytic activity. Mechanistically, liquid-liquid phase separation (LLPS) KAT6A, facilitated APEX1, inhibits cytotoxic effects during treatment. The stable...
Abstract The mechanisms via which inflammatory macrophages mediate intestinal inflammation are not completely understood. Herein, using merged analysis of RNA sequencing and mass spectrometry-based quantitative proteomics, we detected differences between proteomic transcriptomic data in activated macrophages. Dipeptidase-2 (DPEP2), a member the DPEP family, was highly expressed then downregulated sharply at protein level but mRNA response to stimulation. Suppression DPEP2 only enhanced...
Abstract Maintenance of genetic stability via proper DNA repair in stem and progenitor cells is essential for the tissue regeneration, while preventing cell transformation after damage. Loss PUMA dramatically increases survival mice exposure to a lethal dose ionizing radiation (IR), without promoting tumorigenesis long-term survivors. This finding suggests that (p53 upregulated modulator apoptosis) may have function other than regulates apoptosis. Here, we identify novel role regulation...
Langerhans cell histiocytosis (LCH) is the most common with constitutive activation of RAS–RAF–MEK–ERK (MAPKinase) signaling pathway. We analyzed 89 cases BRAF and MAP2K1 mutations by Sanger sequencing, which 18 showed that these two gene are negative. Whole genome sequencing suitable specimens in negative revealed a translocation from 3 intron PLEKHA6 to 13 NTRK3 one case. identified this could cause novel fusion mutation, PLEKHA6‐NTRK3. Overexpression PLEKHA6‐NTRK3 mutant NIH 3T3 cells...
Mechanisms underlying the generation of induced pluripotent stem cells (iPSC) and keeping iPSC stability remain to be further defined. Accumulated evidences showed that reprogramming may controlled by cell-division-rate-dependent model. Here we reported effects absence mouse p27 or p18 on efficiency genomic stability. Expression levels cyclin-dependent kinases inhibitors (CDKIs), p21, p27, decreased during reprogramming. Like p21 loss, deficiency significantly promoted generation, whereas...
Pluripotent stem cells (PSCs) have a unique energetic and biosynthetic metabolism compared with typically differentiated cells. However, the profiling of PSCs its underlying mechanism are still unclear. Here, we report identify purine synthesis enzymes, phosphoribosyl pyrophosphate synthetase 1/2 (PRPS1/2), critical for stemness survival. Ultra-high performance liquid chromatography/mass spectroscopy (UHPLC-MS) analysis revealed that intermediate metabolite levels in higher than somatic...
Macrophage is a very promising cell type for cancer immunotherapy, yet it difficult to obtain enough functional macrophages clinical therapy. Herein, we descibe reliable method produce through the differentiation of human induced pluripotent stem cells (hiPSCs). By optimizing size control embryoid bodies (EBs), accelerated process and increased production without attenuating macrophage functions. Our final yield was close 50-fold starting iPSCs. The showed phagocytic capacity in vitro...
Abstract Patients with relapsed/refractory Burkitt's lymphoma (BL) have a dismal prognosis. Current research efforts aim to increase cure rates by identifying high‐risk patients in need of more intensive or novel therapy. The 8q24 chromosomal translocation the c‐Myc gene, main molecular marker BL, is related metabolism regulating phosphoribosyl pyrophosphate synthetase 2 (PRPS2). In our study, BL showed significant resistance thiopurines. PRPS2 homologous isoenzyme, PRPS1, was demonstrated...
Polydactyly and syndactyly are congenital limb malformations that may occur either as non-syndromic or syndromic forms. In the present study, massively parallel sequencing was performed on a proband in four-generation family with polydactyly to identify disease-causing variant(s). A pathogenic variant c.739C>T (p.Gln247*) glioma-associated oncogene zinc finger 3 (GLI3) gene identified co-segregated affected members of family. Firstly, we examined GLI3 mRNA protein levels peripheral blood...
In the clinic, supply of platelets is frequently insufficient to meet transfusion needs. To address this issue, many scientists have established derivation functional from CD34+ cells or human pluripotent stem (PSCs). However, yield still far below what required. Here we found that plant hormone abscisic acid (ABA) could increase generation megakaryocytes (MKs) and induced PSCs (hiPSCs). During platelet derivation, ABA treatment promoted CD34+/CD45+ HPCs CD41+ MKs on day 14 then increased...
Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and components. However, the mechanisms by which are regulated still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors rabbit oocytes recipients, we show that Oct4 other pluripotency related genes were reactivated in some mouse-rabbit iSCNT embryos, could also activate promoter-driven GFP reporter gene expression. Series...
p53 is one of the most extensively studied proteins in cancer research. Mutations generally abolish normal function, and some mutants can gain new oncogenic functions. However, mechanisms underlying mutation-driven remains to be elucidated. Our study investigated function a heterozygous mutation (p.Asn268Glufs*4) Li-Fraumeni syndrome (LFS) patient. We used episomal technology perform somatic reprogramming, molecular cell biology methods determine levels patient-originated induced pluripotent...