A5055431201- Adipose Tissue and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Diet and metabolism studies
- Adipokines, Inflammation, and Metabolic Diseases
- Dietary Effects on Health
- Regulation of Appetite and Obesity
- Birth, Development, and Health
- Growth Hormone and Insulin-like Growth Factors
- Autophagy in Disease and Therapy
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Sirtuins and Resveratrol in Medicine
- Cell death mechanisms and regulation
- Pancreatic function and diabetes
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Nutrition and Health in Aging
- Circadian rhythm and melatonin
- Calcium signaling and nucleotide metabolism
- Cancer, Hypoxia, and Metabolism
- Digestive system and related health
- Pituitary Gland Disorders and Treatments
- Pain Mechanisms and Treatments
- Liver physiology and pathology
- Ion Channels and Receptors
Tokyo University of Science
2015-2025
The University of Tokyo
2020-2021
Hoshi University
2021
Nagasaki University
2001-2013
Kikkoman (Japan)
2011
University of Wisconsin–Madison
2003-2006
UW Health University Hospital
2003
The University of Texas Health Science Center at San Antonio
1993-1997
University of Otago
1995
Dysfunction of autophagy, which regulates cellular homeostasis by degrading organelles and proteins, is associated with pathogenesis various diseases such as cancer, neurodegeneration metabolic disease. Trehalose, a naturally occurring nontoxic disaccharide found in plants, insects, microorganisms invertebrates, but not mammals, was reported to function mechanistic target the rapamycin (mTOR)-independent inducer autophagy. In addition, trehalose functions an antioxidant though its underlying...
Whether obesity accelerates or suppresses autophagy in adipose tissue is still debatable. To clarify dysregulation of and its role pathologies obese tissue, we focused on lysosomal function, protease maturation activity, both vivo vitro. First, showed that autophagosome formation was accelerated, but autophagic clearance impaired tissue. We also found protein activity levels CTSL (cathepsin L) were suppressed while the CTSB B) significantly enhanced. Moreover, cellular senescence...
We investigated the influences of short-term and lifespan-prolonging long-term caloric restriction (LCR) on gene expression in white adipose tissue (WAT). Over 11,000 genes were examined using high-density oligonucleotide microarrays four groups 10- to 11-month-old male C57Bl6 mice that either fasted for 18 h before death (F), subjected 23 days (SCR), or LCR 9 months compared with nonfasted control (CO) mice. Only a few transcripts F SCR differentially expressed CO In contrast, 345 6,266...
A reduced growth hormone (GH)-insulin-like factor (IGF)-1 axis is associated with an extension of lifespan in laboratory rodents. Several phenotypes such animal models resemble those induced by caloric restriction (CR). Using a transgenic male Wistar rat model whose GH-IGF-1 was moderately suppressed overexpression the antisense GH transgene (tg), we elucidated relationship between effects and CR for some biomarkers aging, lifespan, pathologies. Heterozygous (tg/-) rats fed ad libitum (AL)...
Abstract In C. elegans, insulin‐like hormone signal pathway plays a significant role in longevity. particular, daf‐16 gene product is indispensable factor for this lifespan‐extension. This critical dauer formation, which similar state to hibernation mammals. We examined the expression level of mammalian homologues, Foxo 1,3, and 4 (FKHR, FKHRL1, AFX) mRNAs rat skeletal muscles during aging 30% caloric restricted ad libitum fed. The AFX mRNA was significantly higher at 6 12 months than 3 26...
There has been concern about the suitability of male Fischer 344 (F344) rat as a model for aging research because high prevalence single disease, severe nephropathy, at advanced ages which confounds interpretation an study. In publication from our laboratory, Iwasaki et al. (1988) reported that replacing casein in standard semisynthetic diet with soy protein markedly decreases progression nephropathy advancing age ad libitum fed F344 rats. present study, it is shown lactalbumin does not...
Caloric restriction (CR) can delay onset of several age-related pathophysiologies and extend lifespan in various species, including rodents. CR also induces metabolic remodeling involved activation lipid metabolism, enhancement mitochondrial biogenesis, reduction oxidative stress white adipose tissue (WAT). In studies using genetically modified mice with extended lifespans, WAT characteristics influenced mammalian lifespans. However, molecular mechanisms underlying CR-associated remain...
Cancer cachexia (CC) is a multifactorial disease characterized by decreased food intake and loss of body weight due to reduced musculature with or without fat mass. Patients gastric cancer have high incidence cachexia. We previously established novel CC rat model induced human cancer-derived 85As2 cells in order examine the pathophysiology identify potential therapeutics. In patients CC, anorexia often observed, despite elevation ghrelin, suggesting that ghrelin resistance may develop these...
Abstract Obesity, caused by the dysfunction of white adipose tissue (WAT), is reportedly accompanied exacerbation lipolysis. Perilipin 1 (PLIN1), which forms a coat around lipid droplets, interacts with several lipolysis proteins to regulate While it known that perilipin family are degraded in lysosomes, underlying molecular mechanisms related downregulated expression PLIN1 obese WAT remain unknown. Recently, we found lysosomal originating from an abnormality cathepsin B (CTSB),...
Mesenchymal stem cells (MSCs), which are isolated from adipose tissue (AD-MSCs), umbilical cord (UC-MSCs), or bone marrow, have therapeutic potential including anti-inflammatory and immunomodulatory activities. It was recently reported that MSCs also effective as a treatment for neuropathic pain, although the underlying mechanisms yet to be resolved. Therefore, in this study, we investigated effects of human AD- UC-MSCs on pain its using rat models partial sciatic nerve ligation (PSNL). were...
Cancer cachexia (CC), a syndrome characterized by anorexia and body weight loss due to low fat-free mass levels, including reduced musculature, markedly worsens patient quality of life. Although stomach cancer patients have the highest incidence cachexia, few experimental models for study CC been established. Herein, we developed animal using nude rats subcutaneously implanted with two novel cell lines, i.e., MKN45c185, established from human line MKN-45, 85As2, derived peritoneal...
CHK1 phosphorylates by protein kinase assay (View Interaction: 1, 2, 3)
Now, the quantification of proinsulin/insulin contents within organisms tends to be evaluated only by enzyme-linked immunosorbent assay (ELISA), although assessing adequacy results some method is important. Remarkably, few scientific papers use detection Western blotting (WB), another immunological assay, proinsulin/insulin. We found two problems with insulin and proinsulin general WB: shape an band in gel electrophoresis distorted, retention potency a membrane peptide hormones (mainly...