A5032704177- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Spaceflight effects on biology
- Stress Responses and Cortisol
- Neuroscience of respiration and sleep
- Biochemical Analysis and Sensing Techniques
- Planetary Science and Exploration
- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Receptor Mechanisms and Signaling
- Magnetic and Electromagnetic Effects
- Infant Health and Development
- Biochemical effects in animals
- Adipose Tissue and Metabolism
- Amino Acid Enzymes and Metabolism
- Hypothalamic control of reproductive hormones
- Epigenetics and DNA Methylation
- Photoreceptor and optogenetics research
- Astro and Planetary Science
- Spacecraft and Cryogenic Technologies
- Drug Transport and Resistance Mechanisms
- Dialysis and Renal Disease Management
- Hormonal Regulation and Hypertension
- Growth Hormone and Insulin-like Growth Factors
Chiba University
2021-2025
Institute of Space and Astronautical Science
2009-2024
Japan Aerospace Exploration Agency
2010-2024
Dokkyo Medical University
2018-2023
Oita Medical Center
2017-2021
National Defense Medical College
2021
University of Occupational and Environmental Health Japan
2010-2020
University of Edinburgh
2009-2014
GlaxoSmithKline (Japan)
2014
GlaxoSmithKline (United Kingdom)
2014
The hypothesis called "panspermia" proposes an interplanetary transfer of life. Experiments have exposed extremophilic organisms to outer space test microbe survivability and the panspermia hypothesis. Microbes inside shielding material with sufficient thickness protect them from UV-irradiation can survive in space. This process has been "lithopanspermia," meaning rocky panspermia. We previously proposed sub-millimeter cell pellets (aggregates) could harsh environment based on on-ground...
Ghrelin is known as a potent orexigenic hormone through its action on the brain. In this study, we examined effects of intracerebroventricular (icv) and iv injection ghrelin water intake, food urine volume in rats deprived for 24 h. Water intake that occurred after deprivation was significantly inhibited by icv (0.1, 1, 10 nmol/rat) dose-related manner, although stimulated hormone. The antidipsogenic effect effect. Similarly, inhibited, whereas dose dependently nmol/kg). inhibition drinking...
Cancer cachexia (CC) is a multifactorial disease characterized by decreased food intake and loss of body weight due to reduced musculature with or without fat mass. Patients gastric cancer have high incidence cachexia. We previously established novel CC rat model induced human cancer-derived 85As2 cells in order examine the pathophysiology identify potential therapeutics. In patients CC, anorexia often observed, despite elevation ghrelin, suggesting that ghrelin resistance may develop these...
Here we present a detailed statistical analysis of the discharge characteristics mitral cells main olfactory bulb urethane-anesthetized rats. Neurons were recorded from cell layer, and antidromically identified by stimuli applied to lateral tract. All displayed repeated, prolonged bursts action potentials typically lasting >100 sec separated similarly long intervals; about half completely silent between bursts. No such bursting was observed in nonmitral close proximity cells. Bursts...
Nociceptive stimulation elicits neuroendocrine responses such as arginine vasopressin (AVP) release well activation of the hypothalamo-pituitary-adrenal axis. We have generated novel transgenic rats expressing an AVP–enhanced green fluorescent protein (eGFP) fusion gene, and we examined effects nociceptive on transgene expression in hypothalamus after subcutaneous injection saline or formalin into bilateral hindpaws these rats. assessed (1) AVP levels plasma changes eGFP mRNA heteronuclear...
Cancer cachexia (CC), a syndrome characterized by anorexia and body weight loss due to low fat-free mass levels, including reduced musculature, markedly worsens patient quality of life. Although stomach cancer patients have the highest incidence cachexia, few experimental models for study CC been established. Herein, we developed animal using nude rats subcutaneously implanted with two novel cell lines, i.e., MKN45c185, established from human line MKN-45, 85As2, derived peritoneal...
L-type amino acid transporter 1 (LAT1) is specifically expressed in many malignancies, contributes to the transport of essential acids, such as leucine, and regulates mammalian target rapamycin (mTOR) signaling pathway. We investigated expression profile functional role LAT1 prostate cancer using JPH203, a specific inhibitor LAT1. was highly castration-resistant (CRPC) cells, including C4-2 PC-3 but its level low castration-sensitive LNCaP cells. JPH203 significantly inhibited [
Abstract Monitoring the expression of immediate early genes (IEGs) is useful for following stress‐induced cellular responses in neuroendocrine system. We have examined transcriptional activities four IEGs (c‐ fos , jun B, NGFI‐ A and B) arginine vasopressin (AVP) gene hypothalamic paraventicular (PVN) supraoptic nuclei (SON) rats after acute osmotic stimuli, using situ hybridization histochemistry. After intraperitoneal (i.p.) administration hypertonic saline (2% body weight, 900 mOsm/kg),...
Oxytocin (OXT) is a well-known neurohypophysial hormone that synthesised in the paraventricular (PVN) and supraoptic nuclei (SON) of hypothalamus. The projection magnocellular neurosecretory cells, which synthesise OXT arginine vasopressin PVN SON, to posterior pituitary plays an essential role mammalian labour lactation through its peripheral action. However, previous studies have shown parvocellular OXTergic cells PVN, project medulla spinal cord, are involved various physiological...
Oxytocin (OXT)-containing neurosecretory cells in the parvocellular divisions of paraventricular nucleus (PVN), which project to medulla and spinal cord, are involved various physiological functions, such as sensory modulation autonomic processes. In present study, we examined OXT expression hypothalamo-spinal pathway, well hypothalamo-neurohypophysial system, includes magnocellular PVN supraoptic (SON), after s.c. injection saline or formalin into hindpaws transgenic rats that express...
Radiation dosimetry was carried out at the exposure facility (EF) and pressurized module (PM) of Japanese Kibo installed in International Space Station as one study on environmental monitoring for Tanpopo mission. Three panels three references including biological organic samples luminescence dosimeters were launched to obtain data different durations during 3 years from May 2015 July 2018. The equipped with additional shielding materials (0.55, 2.95, 6.23 g/cm2 mass thickness). relative...
Arginine vasopressin (AVP) and corticotrophin-releasing hormone (CRH) in the parvocellular neurosecretory cells of paraventricular nucleus (PVN) play a major role activating hypothalamic-pituitary-adrenal axis, which is main neuroendocrine response against many kinds stress. We examined effects chronic inflammatory/nociceptive stress on expression AVP-enhanced green fluorescent protein (eGFP) fusion gene hypothalamus, using adjuvant arthritis (AA) model. To induce AA, AVP-eGFP rats were...
Arginine vasopressin (AVP) and corticotrophin‐releasing hormone (CRH) in the parvocellular neurosecretory cells of paraventricular nucleus (PVN) play a major role activating hypothalamic‐pituitary‐adrenal axis, which is main neuroendocrine response against many kinds stress. We examined effects chronic inflammatory/nociceptive stress on expression AVP‐enhanced green fluorescent protein (eGFP) fusion gene hypothalamus, using adjuvant arthritis (AA) model. To induce AA, AVP‐eGFP rats were...
The up-regulation of c-fos gene expression is widely used as a marker neuronal activation elicited by various stimuli. Anatomically precise observation products can be achieved at the RNA level in situ hybridisation or protein immunocytochemistry. Both these methods are time and labour intensive. We have developed novel transgenic rat system that enables trivial visualisation using an enhanced green fluorescent (eGFP) tag. These rats express transgene consisting regulatory sequences drive...
We examined the effects of intracerebroventricular (i.c.v.) administration adrenomedullin 2 (AM2) on plasma oxytocin (OXT) and arginine vasopressin (AVP) levels in conscious rats. Plasma OXT were markedly increased 5 min after i.c.v. AM2 (1 nmol/rat) compared with vehicle remained elevated samples taken at 10, 15, 30, 60 min. By contrast, AVP not significantly between 180 except 30-min time point. Fos-like immunoreactivity (Fos-LI) was observed various brain areas, including paraventricular...
Abstract Purpose: Parathyroid hormone-related protein (PTHrP) is a causative factor of humoral hypercalcemia malignancy (HHM) and concurrent anorexia wasting. Because changes in the expression hypothalamic feeding-regulating peptides can directly affect appetites thereby cause wasting, we addressed whether cachectic syndromes induced by PTHrP rely on action peptides. Experimental Design: Rats were inoculated with LC-6 human cancer xenograft that secreted PTHrP, mRNA levels peptide genes...
Peripheral anorectic hormones, such as glucagon-like peptide (GLP)-1, cholecystokinin (CCK)-8 and leptin, suppress food intake. The newly-identified neuropeptide, nesfatin-1, is synthesised in both peripheral tissues the central nervous system, particularly by various nuclei hypothalamus brainstem. In present study, we examined effects of i.p. administration GLP-1 CCK-8 co-administrations leptin at subthreshold doses confirmed measurement intake, on nesfatin-1-immunoreactive (-IR) neurones...