A5048665689- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Salivary Gland Disorders and Functions
- Oral microbiology and periodontitis research
- Innovative Microfluidic and Catalytic Techniques Innovation
- COVID-19 diagnosis using AI
- Genetic factors in colorectal cancer
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Bacterial Identification and Susceptibility Testing
- Effects of Environmental Stressors on Livestock
- Ruminant Nutrition and Digestive Physiology
- Reproductive Physiology in Livestock
- Respiratory viral infections research
- Oral Health Pathology and Treatment
- Barrier Structure and Function Studies
- Glycosylation and Glycoproteins Research
- Neonatal and Maternal Infections
- Neonatal and fetal brain pathology
- Advanced Sensor and Energy Harvesting Materials
- Drug Transport and Resistance Mechanisms
- Molecular Biology Techniques and Applications
- Advanced Materials and Mechanics
- Microbial Inactivation Methods
Austrian Institute of Technology
2011-2024
Institute of Biosciences and Bioresources
2020
Amsterdam UMC Location Vrije Universiteit Amsterdam
2009
The purpose of the current study was to establish an in vitro model for osteoarthritis (OA) by co-culture osteochondral and synovial membrane explants. Osteochondral explants were cultured alone (control-1) or with (control-2) standard culture medium interleukin-1β (IL1β) tumor necrosis factor (TNFα) added (OA-model-1 = explant; OA-model-2 osteochondroal-synovial explant). In addition, OA-model groups a 2-mm partial-thickness defect created centre cartilage explant. Changes expression...
The RespiDisk platform for automated detection of multiple viral and bacterial respiratory tract infection pathogens.
This study investigated the potential of salivary bacterial and protein markers for evaluating disease status in healthy individuals or patients with gingivitis caries. Saliva samples from caries- gingivitis-free (n = 18), 17), deep caries lesions 38) were collected analyzed 44 candidate biomarkers (cytokines, chemokines, growth factors, matrix metalloproteinases, a metallopeptidase inhibitor, proteolytic enzymes, selected oral bacteria). The resulting data subjected to principal component...
Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients' general health. Early, precise diagnosis personalized monitoring essential for efficient prevention management these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible chair-side use enables analysis non-invasively collected whole saliva samples molecular-based detection ten bacteria: seven...
Goblet cell depletion and down-regulation of MUC2 expression are observed in a significant percentage human non-mucinous colorectal adenocarcinomas. Direct evidence for the role gastrointestinal tumor formation was demonstrated by knockout Muc2 mice that resulted development adenocarcinomas small large intestine. The secretory phospholipase Pla2g2a is protein confers resistance to Apc(Min/+)-induced intestinal tumorigenesis. Like Muc2, intestine exclusively expressed goblet cells Pla2g2a's...
Oral health is maintained by a healthy microbiome, which can be monitored state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (P. gingivalis, T. forsythia, denticola, F. nucleatum, C. rectus, P. intermedia, A. actinomycetemcomitans, S. mutans, sobrinus, associated Lactobacilli) in saliva their clinical status association 214 individuals. Upon examination, subjects were grouped into healthy, caries periodontitis was...
C-reactive protein (CRP) is a commonly used serum biomarker for detecting sepsis in neonates. After the onset of sepsis, serial measurements are necessary to monitor disease progression; therefore, non-invasive detection method beneficial neonatal well-being. While some studies have shown correlation between and salivary CRP levels septic neonates, causal link behind this remains unclear. To investigate relationship, was examined saliva samples from 18 neonates compared with 22 healthy...
The group IIA secretory phospholipase A2 gene, Pla2g2a, confers resistance to intestinal tumorigenesis in the Apc(Min/+) mouse model. However, it is unclear how Pla2g2a exerts its tumor-suppressive effects and whether mode of action depends on Apc-germline mutations.We tested expression a transgene provides protection against carcinogen-induced colon tumors, examined normal microenvironment modulated by expression.Pla2g2a strongly inhibited mice following treatment with DNA alkylating agent...
Background : The group IIA secretory phospholipase A2 gene, Pla2g2a , confers resistance to intestinal tumorigenesis in the Apc Min/+ mouse model. However, it is unclear how exerts its tumor-suppressive effects and whether mode of action depends on Apc-germline mutations. Methods We tested expression a transgene provides protection against carcinogen-induced colon tumors, examined normal microenvironment modulated by expression. Results strongly inhibited mice following treatment with DNA...
This Article presents an automated, compact, and self-contained system for sensitive quantitative detection of blood biomarkers. A disposable microfluidic chip, prefilled with biomarker-specific reagents magnetic beads, can be processed fully automatically by a readout platform, enabling immunoassay-based analysis processing time from sample incubation to signal 20 min. Novel concepts on-chip vortexing the beads reagent storage actuation were developed. lens-free photodiode represents...
As lab-on-a-chip devices aim to incorporate complex and multi-step microfluidic workflows, active fluid control within these platforms is critical. We use a splay aligned liquid crystal network (LCN) photoactuator, capable of deformation upon blue light illumination, as stimuli responsive material create integrated fluidic elements. After optimization the manufacturing parameters, well-defined actuator attained. Illumination with 455 nm emitting diode (LED) at 41 mW cm-2 achieves deflection...
Global healthcare systems are struggling with the enormous burden associated infectious diseases, as well incessant rise of antimicrobial resistance. In order to adequately address these issues, there is an urgent need for rapid and accurate disease diagnostics. The H2020 project DIAGORAS aims at diagnosing oral respiratory tract infections using a fully integrated, automated user-friendly platform physicians' offices, schools, elderly care units, community settings, etc. Oral diseases...
We present an ultrasonic streaming mixer for disposable and on-chip magnetic bead ELISAs. The transducer is placed at system-level to keep cost per chip as low possible, coupled the by means of a solid horn. system provides mixing liquids, well dispersion superparamagnetic beads in ELISA. Additionally it can be used clean chamber surface from nonspecifically bound proteins during washing steps ELISA protocol. Using our time protocol has been greatly reduced down 30 min.
Currently about 30% to 50% of all dairy cows are affected by a metabolic or infectious disease during the transition period. A key factor for preventive actions is ability precisely predict diseases at an early stage. We report longitudinal profile non-esterified fatty acids, beta-hydroxybutyrate (BHB), total bilirubin, and aspartate aminotransferase in hyperketonemic cows. Aiming novel measurement regime improve health cows, we evaluated prognostic classifiers hyperketonemia. In...
A better understanding of the electrodynamic behavior cells interacting with electric fields would allow for novel scientific insights and lead to next generation cell manipulation, diagnostics, treatment. Here, we introduce a promising electrode design by using metal oxide high-k dielectric passivation. The thermally generated passivation layer enables efficient field coupling fluid sample comprising while simultaneously decoupling ohmically from electrolyte, allowing control adjustability...
Capacitive coupling of electric fields diminishes energy dissipation and offers superior control over field parameters, resulting in predictable biological outcomes.
The blood–saliva barrier (BSB) consists of the sum epithelial cell layers oral mucosa and salivary glands. In vitro models BSB are inevitable to investigate understand transport biomarkers from blood saliva. Up now, standardized, line-based epithelium submandibular gland still missing for this purpose. Therefore, we established derived human line HTB-41 (A-253). Single clone isolation resulted in five different clones (B2, B4, B9, D3, F11). Clones were compared parental using measurements...
A microfluidic system for rapid and quantitative detection of plasma biomarkers from patients at intensive care units is presented. The integrates a magnetic bead based immunoassay onto disposable chip. Biomarker concentrations down to 10 pg/mL were detected within 40 minutes with the presented system. Chemiluminescence used as method, which enables signal readout lens-less photodiode optical unit. Due combination an efficient simple chip fabrication, our fulfills requirements potential...
Circulating cell free DNA in serum as well serum-autoantibodies and the proteome have great potential to contribute early cancer diagnostics via non invasive blood tests. However, most preparation protocols destroy protein fraction therefore do not allow subsequent analyses. In this study a novel approach based on methyl binding domain (MBD) is described overcome technical difficulties of combining analysis out one single sample.Serum or plasma samples from 98 control individuals 54 breast...
Currently, subclinical metabolic imbalances at the individual cow and herd level are detected by measuring biomarkers in single blood samples. However, diurnal variations have not been fully described yet but need to be considered when sampling for a robust ad consistent analysis. The study describes influence of lactation phases on circadian rhythms non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHB), total bilirubin (tBIL) aspartate aminotransferase (AST) dairy cows. In an...
We present an endpoint PCR product sensing system for online detection of double-stranded DNA (dsDNA) down to 1 ng/μl in a flow-through design with throughput up 144 samples per hour. The device is assembled from off- the-shelf microfluidic and optical components chamber volume 520 nl without any lenses. Fluorescence realized LED-Photodiode combination, which makes this practicable every lab. direct combination continuous-flow realises integrated diagnostic pathogen detection.
We present a novel method for removing nonspecifically bound proteins on-chip ELISAs (Enzyme-Linked-Immuno-Sorbent-Assays) by integrated ultrasonic washing of the reaction chamber. Low power ultrasound is concentrated into chamber with an horn contained in base measurement system. Application ultrasonication during last step ELISA protocol removes adsorbed from wall while not affecting specifically analytes. This reduces cost disposable assay-chips, delivering comparable results standard...