A5051189521- Invertebrate Immune Response Mechanisms
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- vaccines and immunoinformatics approaches
- Signaling Pathways in Disease
- Enzyme Production and Characterization
- Microfluidic and Bio-sensing Technologies
- Studies on Chitinases and Chitosanases
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
Yale University
2019-2024
Oklahoma State University
2014-2023
China Agricultural University
2016
University of Science and Technology of China
2009-2010
Chinese Academy of Sciences
2010
Hefei National Center for Physical Sciences at Nanoscale
2009
Increasing evidence has suggested that the HIV-1 capsid enters nucleus in a largely assembled, intact form. However, not much is known about how cone-shaped interacts with nucleoporins (NUPs) nuclear pore for crossing complex. Here, we elucidate NUP153 binds by engaging assembled protein (CA) lattice. A bipartite motif containing both canonical and noncanonical interaction modules was identified at C-terminal tail region of NUP153. The cargo-targeting phenylalanine-glycine (FG) engaged CA...
Phenoloxidase (PO)-catalyzed melanization is a universal defense mechanism of insects against pathogenic and parasitic infections. In mosquitos such as Anopheles gambiae, melanotic encapsulation resistance certain parasites that cause malaria filariasis. PO initially synthesized by hemocytes released into hemolymph inactive prophenoloxidase (PPO), which activated serine protease cascade upon recognition foreign invaders. The mechanisms PPO activation catalysis have been elusive. Herein, we...
Abstract COVID-19 pathogen SARS-CoV-2 has infected hundreds of millions and caused over 5 million deaths to date. Although multiple vaccines are available, breakthrough infections occur especially by emerging variants. Effective therapeutic options such as monoclonal antibodies (mAbs) still critical. Here, we report the development, cryo-EM structures, functional analyses mAbs that potently neutralize variants concern. By high-throughput single cell sequencing B cells from spike receptor...
COVID-19 pathogen SARS-CoV-2 has infected hundreds of millions and caused over 5 million deaths to date. Although multiple vaccines are available, breakthrough infections occur especially by emerging variants. Effective therapeutic options such as monoclonal antibodies (mAbs) still critical. Here, we report the development, cryo-EM structures, functional analyses mAbs that potently neutralize variants concern. By high-throughput single cell sequencing B cells from spike receptor binding...
Rtt106p is a Saccharomyces cerevisiae histone chaperone with roles in heterochromatin silencing and nucleosome assembly. The molecular mechanism by which engages chromatin dynamics remains unclear. Here, we report the 2.5 A crystal structure of core domain Rtt106p, adopts an unusual "double pleckstrin homology" architecture that represents novel structural mode for chaperones. H3-H4-binding region double-stranded DNA-binding have been identified. Mutagenesis studies reveal DNA binding...
In archaea and eukaryotes, the nascent polypeptide-associated complex (NAC) is one of cytosolic chaperones that contact polypeptide chains as they emerge from ribosome assist in post-translational processes. The eukaryotic NAC a heterodimer, its two subunits form stable through dimerizing domain called domain. addition to acting protein translation chaperone, also function individually transcriptional regulation. Here we report crystal structure human domain, which reveals manner...
COVID-19 is a global crisis of unimagined dimensions. Currently, Remedesivir only fully licensed FDA therapeutic. A major target the vaccine effort SARS-CoV-2 spike-hACE2 interaction, and assessment efficacy relies on time consuming neutralization assay. Here, we developed cell fusion assay based upon interaction. The system was tested by transient co-transfection 293T cells, which demonstrated good correlation with standard spike pseudotyping for inhibition sera biologics. Then established...
APOBEC3 (A3) proteins are a family of host antiviral restriction factors that potently inhibit various retroviral infections, including human immunodeficiency virus (HIV)-1. To overcome this restriction, HIV-1 virion infectivity factor (Vif) recruits the cellular cofactor CBFβ to assist in targeting A3 E3 ligase complex for polyubiquitination and subsequent proteasomal degradation. Intervention Vif-A3 interactions could be promising therapeutic strategy facilitate A3-mediated suppression...
The apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3 (APOBEC3 or A3) family of proteins functions in the innate immune system. A3 are interferon inducible and hypermutate deoxycytidine to deoxyuridine foreign single-stranded DNA (ssDNA). However, this deaminase activity cannot discriminate between host ssDNA at biochemical level, which presents a significant danger when gain access nucleus. Interestingly, capability can be harnessed coupled with novel CRISPR-Cas9 create...
Summary The capsid of human immunodeficiency virus 1 (HIV-1) plays a pivotal role in viral nuclear import, but the mechanism by which core passages pore complex (NPC) is poorly understood. Here, we use DNA-origami mimics NPC, termed NuPODs (NucleoPorins Organized DNA), to reveal mechanistic underpinnings HIV-1 entry. We found that trimeric interface formed via three protein hexamers targeted triple-arginine (RRR) motif not canonical phenylalanine-glycine (FG) NUP153. As NUP153 located on...
Summary The COVID-19 pandemic affects millions of people worldwide with a rising death toll. causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), uses its nonstructural protein 1 (Nsp1) to redirect host translation machinery the viral RNA by binding ribosome and suppressing cellular, but not viral, synthesis through yet unknown mechanisms. We show here that among all proteins, Nsp1 has largest impact on viability in cells human lung origin. Differential expression...
The SARS-CoV-2 variant, Omicron (B.1.1.529), rapidly swept the world since its emergence. Compared with previous variants, has a high number of mutations, especially those in spike glycoprotein that drastically dampen or abolish efficacy currently available vaccines and therapeutic antibodies. Several major sublineages evolved, including BA.1, BA.1.1, BA.2, BA.2.12.1, BA.3, BA.4/5, BA.2.75, which changing global regional landscape pandemic. Although are available, antibodies remain critical...