A5051102919- Nitric Oxide and Endothelin Effects
- Neuroscience of respiration and sleep
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Renin-Angiotensin System Studies
- Electron Spin Resonance Studies
- Eicosanoids and Hypertension Pharmacology
- Sulfur Compounds in Biology
- Neonatal Respiratory Health Research
- Neonatal and fetal brain pathology
- Adolescent Sexual and Reproductive Health
- Cardiac Ischemia and Reperfusion
- Neonatal Health and Biochemistry
- Adipose Tissue and Metabolism
- Hemoglobin structure and function
- HIV, Drug Use, Sexual Risk
- Hemoglobinopathies and Related Disorders
- HIV/AIDS Research and Interventions
- Gallbladder and Bile Duct Disorders
- Metabolism and Genetic Disorders
- Physiological and biochemical adaptations
- Cancer therapeutics and mechanisms
- Phytochemistry and Bioactive Compounds
- Botanical Studies and Applications
- Abdominal Trauma and Injuries
Loma Linda University
2014-2025
University of Alabama at Birmingham
2018
University of California, Riverside
2018
Harbin Medical University
2017
Second Affiliated Hospital of Harbin Medical University
2017
Sichuan University
2007-2009
Objectives: To find and compare the levels of acceptance barriers to voluntary counselling testing (VCT) among adults in two different counties Guizhou province, China, one which China CARES project was operating other it not. Design: A longitudinal design with two-stage cluster sampling employed. Methods: total 1012 participants were recruited counties. All interviewed, then given a coupon for free VCT after interview. Participants paid returning within 2 months, whether tested or The...
L-NG-Nitro arginine methyl ester (L-NAME) has been widely applied for several decades in both basic and clinical research as an antagonist of nitric oxide synthase (NOS). Herein, we show that L-NAME slowly releases NO from its guanidino nitro group. Daily pretreatment rats with potentiated mesenteric vasodilation induced by nitrodilators such nitroglycerin, but not NO. Release also occurred the NOS-inactive enantiomer D-NAME, L-arginine or another NOS inhibitor L-NMMA, consistent presence...
Nitrite can be converted to nitric oxide (NO) by a number of different biochemical pathways. In newborn lambs, an aerosol inhaled nitrite has been found reduce pulmonary blood pressure, possibly acting via conversion NO reaction with intraerythrocytic deoxyhemoglobin. If so, the vasodilating effects would attenuated free hemoglobin in plasma that rapidly scavenge NO.Pulmonary vascular pressures and resistances flow were measured anesthetized lambs. Plasma concentrations then elevated,...
Glutathione-liganded binuclear dinitrosyl iron complex (glut-BDNIC) has been proposed to be a donor of nitric oxide (NO). This study was undertaken investigate the mechanisms vasoactivity, systemic hemodynamic effects, and pharmacokinetics glut-BDNIC. To test hypothesis that glut-BDNICs vasodilate by releasing NO in its reduced [nitroxyl (HNO)] state, bioassay method isolated, preconstricted ovine mesenteric arterial rings used presence selective scavengers HNO or free radical...
Abstract Although the gasotransmitter hydrogen sulfide (H 2 S) is well known for its vasodilatory effects, H S also exhibits vasoconstricting properties. Herein, it demonstrated that administration of as intravenous sodium (Na increased blood pressure in sheep and rats, this effect persisted after has disappeared from blood. Inhibition L‐type calcium channel (LTCC) diminished hypertensive effects. Incubation Na with whole blood, red cells, methemoglobin, or oxyhemoglobin produced a product...
Fetal cerebral blood flow increases in response to acute hypoxia, mediated part by an adrenergic α1 receptor (α1-R)-mediated increase peripheral vascular resistance that redirects cardiac output the brain. Activation of α1-R may attenuate during and this effect be even greater fetuses exposed chronic high-altitude previously shown contractile function artery α1-Rs. We hypothesized activation fetal sheep brain attenuates hypoxia would accentuated hypoxia. Near-term gestated at low or high...
BACKGROUND: Pulmonary hypertension of the newborn is a life-threatening disorder characterized by elevated pulmonary vascular resistance due to maladaptation circulation after birth. The etiology and mechanisms underlying remain unclear, hindering development effective treatment. We hypothesize that perinatal chronic hypoxia upregulates microRNA-210, which essential for suppression arterial spontaneous transient outward currents (STOCs), resulting in newborn. METHODS: tested this hypothesis...
Nitrosyl heme (heme-NO) has recently emerged as a surrogate signaling entity for nitric oxide (NO). However, questions remain about how heme-NO signals across the cell membrane. Herein, we test hypothesis that nitrodilator vasodilates by mobilizing NO moiety from nitrodilator-activated intracellular store (NANOS) in vasculature. We identify novel mechanism glutathione-catalyzed formation of model compound, alb-heme-NO, and determine glutathione (GSH) ligand its structure. Heme-NO complexes...
Nitric oxide (NO) is a gasotransmitter with important physiological and pathophysiological roles in pregnancy. There limited information available about the sources metabolism of NO its bioactive metabolites (NOx) both normal complicated pregnancies. The present study characterized quantified endogenous NOx human mouse placenta following determination stability exogenous placental homogenates. have differential iron nitrosyl species (FeNOs), are relatively unstable homogenates from...
Background There is evidence from various models of hypoxic-ischemic injury (HII) that nitric oxide (NO) protective. We hypothesized either inhaled NO (iNO) or nitrite would alleviate brain in neonatal HII via modulation mitochondrial function. Methods tested the effects iNO and on Rice-Vannucci model 7-day-old rats. Brain mitochondria were isolated for flow cytometry, aconitase activity, electron paramagnetic resonance, Seahorse assays. Results Pretreatment pups with decreased survival HII,...
Recent evidence from humans and rats indicates that nitrite is a vasodilator under hypoxic conditions by reacting with metal-containing proteins to produce nitric oxide (NO). We tested the hypothesis near-physiological concentrations of would vasodilation in hypoxia- concentration-dependent manner hind limb sheep. Anesthetized sheep were instrumented measure arterial blood pressure femoral flows continuously both limbs. Nitrite was infused into one artery raise concentration vein 10 15-fold...
Placental nitric oxide (NO) is critical for maintaining perfusion in the maternal-fetal-placental circulation during normal pregnancy. NO and its many metabolites are also increased pregnancies complicated by maternal inflammation such as preeclampsia, fetal growth restriction, gestational diabetes, bacterial infection. However, it unclear how levels of or affect placental function placenta deals with excessive metabolites. Since there uncertainty over direction change plasma we measured...
Key points Recent evidence in adult humans demonstrates that nitrite, at physiological concentrations, can be converted into vasodilating amounts of NO, thus constituting an alternative to NO production by synthases. Nitrite reacts with deoxyhaemoglobin produce a reaction proposed mediate the effects nitrite. We have demonstrated previously rate this is ∼2‐fold faster fetal haemoglobin than haemoglobin. Thus, we hypothesized nitrite would potent vasodilator cephalic vasculature served...
We recently developed a combination of four chemiluminescence-based assays for selective detection different nitric oxide (NO) metabolites, including nitrite, S-nitrosothiols (SNOs), heme-nitrosyl (heme-NO), and dinitrosyl iron complexes (DNICs). However, these NO species (NOx) may be under dynamic equilibria during sample handling, which affects the final determination made from readout assays. Using fetal maternal sheep low high altitudes (300 3801 m, respectively) as models NOx levels...
A cross-sectional study employing two-stage cluster sampling was conducted between December 2005 and March 2006 to compare adults' knowledge of HIV/AIDS voluntary counseling testing (VCT) the attitudes toward acceptance VCT a county in which comprehensive program, China CARES (CC), available, where it not. Information on knowledge, awareness VCT, collected. All participants were given coupon for free after interview. Uptake measured within 2 months More CC knew someone infected with HIV, had...
Circulating metabolites of nitric oxide, such as nitrite, iron nitrosyls (FeNO), and nitrosothiols, have vasodilatory bioactivity. In both human sheep neonates, plasma concentrations these NO metabolite (NOx) fall >50% within minutes after birth, raising the possibility that circulating NOx plays a role in maintaining low fetal vascular resistance cardiovascular transition at birth. To test whether birth is due to either ligation umbilical cord or oxygenation fetus newborn levels, were...