A5075195393- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Lipid metabolism and biosynthesis
- Gut microbiota and health
- Genetic and phenotypic traits in livestock
- Fatty Acid Research and Health
- Genetic Syndromes and Imprinting
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Clostridium difficile and Clostridium perfringens research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Molecular Biology Techniques and Applications
- Peroxisome Proliferator-Activated Receptors
- Cancer-related gene regulation
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Pancreatic function and diabetes
- Milk Quality and Mastitis in Dairy Cows
- Wnt/β-catenin signaling in development and cancer
Jiangsu University
2020-2024
Nanjing Agricultural University
2013-2020
National Bureau of Statistics of China
2002
The aim of this study was to determine the effects long-term Nicotinamide mononucleotide (NMN) treatment on modulating gut microbiota diversity and composition, as well its association with intestinal barrier function. In study, C57BL/6J mice were fed different concentrations NMN, their feces collected for detection 16S rDNA non-targeted metabolites explore NMN metabolites. results revealed that increased abundance butyric acid-producing bacteria (Ruminococcae_UCG-014...
Reproductive ability, especially prolificacy, impacts sheep profitability. Hu sheep, a unique Chinese breed, is recognized for its high prolificacy (HP), early sexual maturity, and year-round estrus. However, little known about the molecular mechanisms underlying HP in sheep. To explore potential mRNAs long non-coding RNAs (lncRNAs) involved we performed an ovarian genome-wide analysis of lncRNAs during follicular stage using (litter size = 3; three consecutive lambings) low (LP, litter 1;...
Long noncoding RNAs (LncRNAs) have been identified as important regulators of testis development; however, their expression patterns and roles in sheep are not yet clear. Thus, we used stranded specific RNA-seq to profile the transcriptome (lncRNAs mRNAs) premature mature sheep. Hormone levels index were examined, histological analyses performed at five stages development, 5-day-old (D5), 3-month-old (3M), 6-month-old (6M), 9-month-old (9M), 2-year-old (2Y), results which indicate a...
Long non-coding RNA (LncRNA) have been identified as important regulators in the hypothalamic-pituitary-ovarian axis associated with sheep prolificacy. However, their expression pattern and potential roles pituitary are yet unclear. To explore mRNAs lncRNAs that regulate of genes involved prolificacy, we used stranded specific RNA-seq to profile transcriptome (lncRNA mRNA) high prolificacy (genotype FecB BB, litter size = 3; H) low B+; 1; L). Our results showed 57 differentially expressed...
Nicotinamide mononucleotide (NMN) exerts physiological effects in mammals through its conversion to nicotinamide adenine dinucleotide (NAD+). In this study, we established experimental models of colitis by mixing drinking water C57BL/6J mice with dextran sodium sulphate (DSS), and then fed them the same concentration NMN or at time. After treatment, observed improved morphology inflamed intestines, slightly restored length colon, barrier function reduced proinflammatory factors expression...
Abstract Spermatogenesis can be affected by nutrition, which operates through normal physiological processes changing the testicular mass and hormone levels profoundly. However, little is known regarding how testis development regulated long noncoding RNA (lncRNA). In this study, we investigated effects of high-grain (HG) feeding on during sexual maturation mediated lncRNA. The HG diet group showed an increase in growth (GH), insulin-like factor-1 (IGF-1) testosterone (T) levels, number...
In mammals, their proper development during the early cleavage stages strongly relies on gene products newly transcribed by zygotic genome activation (ZGA). Long noncoding RNAs (lncRNAs) have been characterized as key regulators of ZGA process in mice and human. However, stage has not yet identified epigenetic regulations remain largely unknown goats. Here, we show that occurred at 8-cell During ZGA, trimethylation H3K9 was dynamically changed but maintained strong staining arrested embryos....
As an important type of noncoding RNA molecules, long non-coding RNAs (lncRNAs) act as versatile players in various biological processes. However, little is known about lncRNA regulators during sheep muscle growth. To explore functional lncRNAs growth, we systematically investigated using strand-specific Ribo-Zero sequencing at three key developmental stages Hu sheep. A total 6924 were obtained, and the differentially expressed genes screened from (control vs. experiment) fetus lamb, lamb...
Preventive effects of Lactobacillus plantarum CBT against colorectal tumorigenesis by regulating arginine metabolism in gut microbiota.
Abstract Adipose tissue development is regulated by a serial of developmental signaling pathways. The Hippo pathway novel cascade closely associated with adipogenesis. While most components had been verified that have vital role in preadipocytes proliferation and differentiation, little known about the function Yes‐associated protein 1 (YAP1) mammalian adipose development. Therefore, we investigated YAP1 ovine vitro vivo experiments. We observed adipocyte size subcutaneous increased...
The present study aimed to evaluate the impact of hydroxychloroquine (HCQ) on mucosal barrier and gut microbiota during healing mice colitis.
Ythdf2 is known to mediate mRNA degradation in an m6A-dependent manner, and it has been shown play a role skeletal muscle differentiation. Recently, was also found bind m6A-modified precursor miRNAs regulate their maturation. However, remains unknown whether this mechanism related the regulation of myogenesis by Ythdf2. Here, we observed that knockdown significantly suppressed myotube formation impacted expression during myogenic Through integrated analysis miRNA sequencing data, miR-378...
Runt-related transcription factor 1 translocation partner (RUNX1T1), a potential novel regulator of adipogenesis, exists in two splice variants: long (RUNX1T1-L) and short (RUNX1T1-S) isoform. However, there is no data showing the existence RUNX1T1 ovine subcutaneous fat at different stages developmental its role on adipogenesis. Therefore, objectives this study were to evaluate presence five-day-old 24-month-old sheep investigate In study, we detected 1829 bp cDNA fragment which contains...
AlkB homolog 5, RNA demethylase (ALKBH5) is abnormally highly expressed in glioblastoma multiforme (GBM) and negatively correlated with overall survival GBM patients.In this study, we found a new mechanism that ALKBH5 pyrroline-5-carboxylate reductase 2 (PYCR2) formed positive feedback loop involved proline synthesis GBM.ALKBH5 promoted PYCR2 expression PYCR2-mediated synthesis; while through the AMPK/mTOR pathway cells.In addition, cell proliferation, migration, invasion, as well...
The objective of this study was to investigate the dose-dependent effect 1α,25-(OH)2VD3 (Vit D3) on invitro proliferation goat luteinised granulosa cells (LGCs) and determine underlying mechanisms its action by overexpressing silencing vitamin D receptor (VDR) in LGCs. Results showed that VDR prominently localised GCs theca (TCs) expression increased with follicle diameter, but lower atretic follicles than healthy follicles. rate LGCs significantly higher Vit D3-treated groups control group,...
Mitophagy, the cellular process that removes damaged mitochondria, plays a crucial role in maintaining normal cell functions. It is deeply involved entire of follicle development and associated with various ovarian diseases. This review aims to provide comprehensive overview mitophagy regulation, emphasizing its at different stages follicular development. Additionally, study illuminates relationship between diseases, including ovary aging (OA), primary insufficiency (POI), polycystic...
Somatic cell nuclear transfer (SCNT) is a useful way to produce cloned animals. However, SCNT animals exhibit DNA methylation and genomic imprinting abnormalities. These abnormalities may be due the faulty epigenetic reprogramming of donor cells. To investigate consequence on global in cells, growth patterns apoptosis goat fibroblast cells (CGFCs) at passage 7 were determined. Growth CGFCs similar controls; however, rate log phase was lower significantly higher (P < 0.01). In addition,...
Sterol‐CoA desaturase 1 ( Scd1 ) plays a pivotal role in the synthesis and metabolism of fatty acids (FA). However, whether contributes to lipid accumulation dairy goat mammary epithelial cells (GMEC), how it affects FA remains unexplored. The aim this study was detect effect on droplets (LD) formation uncover its regulatory mechanism GMEC. In study, novel fluorescence reporter system developed confirm expression SCD1 protein GMEC plasma. addition, mammalian vector (pCAG‐SCD1) siRNA...