Lipid bilayers immobilized in planar geometries, such as solid-supported or "floating" bilayers, have enabled detailed studies of biological membranes with numerous experimental techniques, notably X-ray and neutron reflectometry. However, the presence a solid support also has disadvantages it complicates use spectroscopic techniques well surface rheological measurements that would require deformations. Here, order to overcome these limitations, we investigate lipid adsorbed inherently soft...

Many organisms rely on mineral nutrients taken directly from the soil or aquatic environment, and therefore, developed mechanisms to cope with limitation of a given essential nutrient. For example, photosynthetic cells have well-defined responses phosphate limitation, including replacement cellular membrane phospholipids non-phosphorous lipids. Under starvation, in extraplastidial membranes are replaced by betaine lipids microalgae. In higher plants, synthesis lipid is lost, driving plants...

Glycolipids are known to stabilize biomembrane multilayers through preferential sugar–sugar interactions that act as weak transient membrane cross-links. Here, we use small-angle and quasi-elastic neutron scattering on oligolamellar phospholipid vesicles containing defined glycolipid fractions in order elucidate the influence of glycolipids mechanics dynamics. Small-angle (SANS) reveals (OLVs) obtained by extrusion polydisperse with regard number lamellae, n, which renders interpretation...

We investigate the interaction between highly charged lipid bilayers in presence of monovalent counterions. Neutron and X-ray reflectivity experiments show that water layer like-charged is thinner than for zwitterionic lipids, demonstrating existence counterintuitive electrostatic attractive them. Such attraction can be explained by taking into account correlations counterions within Strong Coupling limit, which falls beyond classical Poisson–Boltzmann theory electrostatics. Our results...

Glycolipids affect the inter-bilayer water layer thickness and contribute to stability of double bilayer architecture through formation “sugar bonds”.

Theoretical simulations have predicted that a lipid bilayer forms stable superstructure when sheet of graphene is inserted in its hydrophobic core. We experimentally produced for the first time lipid–graphene–lipid assembly by combining Langmuir–Blodgett and Langmuir–Schaefer methods. Graphene sandwiched remains flat within core bilayer. Using infrared spectroscopy, ellipsometry, neutron reflectometry, we characterized at every fabrication step. The hybrid mechanically does not disturb...

The stratum corneum (SC) is the largest physical barrier of human body. It protects against physical, chemical and biological damages, avoids evaporation water from deepest skin layers. For its correct functioning, homeostasis SC lipid matrix fundamental. An alteration composition in particular ceramide (CER) fraction can lead to development pathologies such as atopic dermatitis psoriasis. Different studies showed that direct replenishment lipids on damaged had positive effects recovery...

Interactions of anions with hydrophobic surfaces proteins and water-soluble polymers depend on the ability ions to shed their hydration shells. At positively charged surfactant monolayers, interactions are less well understood. Due interplay electrostatic surface forces, effects, ion–ion in double layer, a comprehensive microscopic picture remains elusive. Herein, we study chloride, bromide, mixture these two at aqueous interface dihexadecyldimethylammonium (DHDA+)...

In certain bacteria, phosphatidylethanolamine lipids (PEL) get largely replaced by phosphate-free ornithine (OL) under conditions of phosphate starvation. It has so far been unknown how much these two lipid types deviate in their physicochemical properties, and strongly bacteria thus have to adapt order compensate for the difference. Here, we use differential scanning calorimetry, X-ray scattering, fluorescence investigate properties OL with saturated C14 alkyl chains mono- bilayers. is...

The stratum corneum represents the first skin barrier against chemical and physical damage. These unique properties are based on its peculiar lipid composition with ceramides (CERs) as main protagonists. In this study, structural of α-OH phytosphingosine [AP] CER class have been investigated. CERs present in their d-forms; however, most model systems diastereomer mixture synthetically produced l-form is used. d-form well-known to form a hydrogen bonding network that helps reduce permeability...

Glycolipids are known to stabilize biomembrane multilayers through preferential sugar-sugar interactions that act as weak transient membrane cross-links. Here, we use small-angle and quasi-elastic neutron scattering on oligolamellar phospholipid vesicles containing defined glycolipid fractions in order elucidate the influence of glycolipids mechanics dynamics. Small-angle (SANS) reveals (OLVs) obtained by extrusion polydisperse with regard number lamellae, n, which renders interpretation...

Langmuir monolayers of chiral amphiphiles are well-controlled model systems for the investigation phenomena related to stereochemistry. Here, we have investigated mixed one pair enantiomers (l and d) amino-acid-based amphiphile N-stearoyl-threonine. The monolayer characteristics were studied by pressure–area isotherm measurements grazing incidence X-ray diffraction (GIXD) over a wide range mixing ratios defined d-enantiomer mole fraction xD. While isotherms provide insights into...

Recent studies on Escherichia coli bacteria cultivation, in which DNA thymine was replaced with 5-chlorouracil have refreshed the problem of understanding changes to physical properties monomers resultant from chemical modifications. These shown that replacement did not affect normal activities and division bacteria, but has significantly reduced its life span. In this paper a comparative analysis carried out by methods computational experiment set 687 possible conformers natural monomeric...

Lipid bilayers immobilized in planar geometries, such as solid-supported or "floating" bilayers, have enabled detailed studies of biological membranes with numerous experimental techniques, notably x-ray and neutron reflectometry. However, the presence a solid support also has disadvantages it complicates use spectroscopic techniques well surface rheological measurements that would require deformations. Here, order to overcome these limitations, we investigate lipid adsorbed inherently soft...

This article shows how to change the color of metal and glass. Both these materials are self–sufficient, but sometimes used together. For example, enameling. In this case, adhesion between substrate stekloobraznae enamel layer, which was conducted on a stretching bend, tested.

ABSTRACT Phosphate is vital for plant and algae growth, yield, survival, but in most environments, it poorly available. To cope with phosphate starvation, photosynthetic organisms used their phospholipids as a reserve. In microalgae, betaine lipids replace whereas, higher plants, lipid synthesis lost, driving plants to other strategies. The aim of this work was evaluate what extent PC share physicochemical properties could thus substitute each other. Using neutron diffraction molecular...

Glycolipids are known to be involved in the formation of ordered functional domains biological membranes. Since structural characterization such is difficult, most studies have so far dealt with lipid mixtures containing only one glycolipid component at a time, although membranes usually contain several species, which can result more complex structures and phase behavior. Here, we combine classical isotherm measurements surface-sensitive grazing-incidence X-ray diffraction investigate...