A5083167844- Animal testing and alternatives
- Effects and risks of endocrine disrupting chemicals
- Immunotoxicology and immune responses
- Agricultural safety and regulations
- Health Systems, Economic Evaluations, Quality of Life
- Contact Dermatitis and Allergies
- 3D Printing in Biomedical Research
- Pesticide Exposure and Toxicity
- Computational Drug Discovery Methods
- Environmental Toxicology and Ecotoxicology
- Carcinogens and Genotoxicity Assessment
- Biomedical Ethics and Regulation
- Meta-analysis and systematic reviews
- Anesthesia and Neurotoxicity Research
- Pluripotent Stem Cells Research
- Glaucoma and retinal disorders
- Chemical Analysis and Environmental Impact
- Marine Biology and Environmental Chemistry
- Advancements in Transdermal Drug Delivery
- Risk and Safety Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Ocular and Laser Science Research
- Phytochemistry Medicinal Plant Applications
- Radioactive Decay and Measurement Techniques
European Food Safety Authority
2022-2025
FORS – Swiss Centre of Expertise in the Social Sciences
2019
European Commission
2013
Joint Research Centre
2002-2009
University of Lausanne
1999-2002
ECVAM sponsored a formal validation study on three in vitro tests for skin irritation, of which two employ reconstituted human epidermis models (EPISKIN™, EpiDerm™), and one, the integrity function test (SIFT), employs ex vivo mouse skin. The goal was to assess whether would correctly predict classifications according EU classification scheme, “R38” “no label” (i.e. non-irritant). 58 chemicals (25 irritants 33 non-irritants) were tested, having been selected give broad coverage...
Integrated testing strategies (ITS), as opposed to a single definitive test or fixed batteries of tests, are expected efficiently combine different information sources in quantifiable fashion satisfy an need, this case for regulatory safety assessments. With increasing awareness the limitations each individual tool and development highly targeted tests predictions, need combining pieces evidence increases. The discussions that took place during workshop, which brought together group experts...
For more than two decades, scientists have been trying to replace the regulatory in vivo Draize eye test by vitro methods, but so far only partial replacement has achieved. In order better understand reasons for this, historical rabbit data were analysed detail and resampled with purpose of (1) revealing which endpoints are most important driving United Nations Globally Harmonized System/European Union Regulation on Classification, Labelling Packaging (UN GHS/EU CLP) classification serious...
Abstract EFSA was asked for a scientific opinion on the risks animal and human health related to presence of polychlorinated naphthalenes (PCNs) in feed food. The assessment focused hexaCNs due very limited data other PCN congeners. For feed, 217 analytical results were used estimate dietary exposures food-producing non-food-producing animals; however, risk characterisation could not be performed because none toxicological studies allowed identification reference points. oral repeated dose...
Abstract Microglial cells react early to a neurotoxic insult. However, the bioactive factors and cell‐cell interactions leading microglial activation finally neuroprotective or neurodegenerative outcome remain be elucidated. Therefore, we analyzed reaction induced by methylmercury (MeHgCl) using cell cultures of different complexity. Isolated miroglia were found directly activated MeHgCl (10 −10 10 −6 M), as indicated process retraction, enhanced lectin staining, cluster formation. An...
Abstract Trimethyltin (TMT) is a neurotoxicant known to induce early microglial activation. The present study was undertaken investigate the role played by these cells in TMT‐induced neurotoxicity. effects of TMT were investigated monolayer cultures isolated microglia or neuron‐enriched and neuron–microglia astrocyte–microglia cocultures. end points used morphological criteria; evaluation cell death proliferation; measurements tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), nitric...
Abstract The European Commission asked EFSA to update its 2014 risk assessment on perchlorate in food. Perchlorate is a contaminant of both natural and anthropogenic sources present food drinking water. It substrate for the sodium iodide symporter (NIS) competitively inhibits uptake into thyroid. Experimental animal studies show that exposure during pregnancy can result neurodevelopmental toxicity. CONTAM Panel established tolerable daily intake 1.4 μg/kg body weight per day, based...
Abstract Despite a wealth of data on the neurotoxic effects lead at cellular and molecular levels, reasons for its development‐dependent neurotoxicity are still unclear. Here, maturation‐dependent acetate were analyzed in immature differentiated brain cells cultured aggregates. Markers general cytotoxicity as well cell‐type‐specific markers glial neuronal showed that more sensitive to than counterparts, demonstrating can be reproduced aggregating cell cultures. After 10 days treatment,...
The ECVAM-funded skin irritation validation study (SIVS) was initiated in 2003, with the aim to evaluate whether EpiDerm, EPISKIN and SIFT alternative methods were able reliably identify irritant non-irritant chemicals, could therefore be candidates for replacing rabbit Draize test irritation. primary goal of predictive capacity assays regard EU classification system, which employs risk phrases, "R38", irritants, "no label" non-irritants. A secondary objective retrospective analysis data,...