A5034490340- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Mitochondrial Function and Pathology
- interferon and immune responses
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Chronic Lymphocytic Leukemia Research
- Telomeres, Telomerase, and Senescence
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Selenium in Biological Systems
- Osteoarthritis Treatment and Mechanisms
- Advanced Breast Cancer Therapies
- Ferroptosis and cancer prognosis
- Connective Tissue Growth Factor Research
- Solar-Powered Water Purification Methods
- DNA Repair Mechanisms
- Amino Acid Enzymes and Metabolism
- Thermal properties of materials
- Retinoids in leukemia and cellular processes
- Nanopore and Nanochannel Transport Studies
- Inflammasome and immune disorders
Instituto de Investigación Biomédica de A Coruña
2015-2025
Servicio Gallego de Salud
2015-2025
Complexo Hospitalario Universitario A Coruña
2015-2025
Universidade da Coruña
2015-2025
Queen Mary University of London
2017-2022
Newark Hospital
2018-2022
Centro de Investigación Biomédica en Red
2014-2017
Radboud University Medical Center
2017
Radboud University Nijmegen
2017
Senescence is a cellular phenotype present in health and disease, characterized by stable cell-cycle arrest an inflammatory response called senescence-associated secretory (SASP). The SASP important influencing the behavior of neighboring cells altering microenvironment; yet, this role has been mainly attributed to soluble factors. Here, we show that both factors small extracellular vesicles (sEVs) are capable transmitting paracrine senescence nearby cells. Analysis individual internalizing...
Aging is a process of cellular and tissue dysfunction characterized by different hallmarks, including senescence. However, there proof that certain features aging senescence can be ameliorated. Here, we provide evidence small extracellular vesicles (sEVs) isolated from primary fibroblasts young human donors ameliorate biomarkers in cells derived old Hutchinson-Gilford progeria syndrome donors. Importantly, sEVs variety tissues mice. Mechanistically, identified to have intrinsic...
Abstract Stem cells possess significant age-dependent differences in their immune-response profile. These were analysed by Next-Generation Sequencing of six age groups from bone marrow mesenchymal stem cells. A total 9,628 genes presenting differential expression between grouped into metabolic pathways. We focused our research on young, pre-pubertal and adult groups, which presented the highest amount differentially expressed related to inflammation mediated chemokine cytokine signalling...
Senescent cells accumulate in several tissues during ageing and contribute to pathological processes such as cancer. Senescence induction is a complex process not well defined yet characterized by series of molecular changes acquired after an initial growth arrest. We found that fatty acid synthase (FASN) levels increase the senescence mouse hepatic stellate human primary fibroblasts. Importantly, we also observed significant FASN liver tissues. To probe central role induction, used...
Abstract Mesenchymal stem cells promising role in cell-based therapies and tissue engineering appears to be limited due a decline of their regenerative potential with increasing donor age. Six age groups from bone marrow mesenchymal Wistar rats were studied (newborn, infant, young, pre-pubertal, pubertal adult). Quantitative proteomic assay was performance by iTRAQ using an 8-plex labeling the proteins differentially expressed grouped pluripotency, proliferative metabolism processes....
Abstract Introduction This study assessed whether mesenchymal stem cell (MSC)-derived extracellular vesicles influenced ageing and pluripotency markers in cultures where they are added. Methods MSC-derived from old young rat bone marrows were isolated by ultracentrifugation characterised western blotting, nanoparticle tracking analysis (NTA) transmission electron microscopy (TEM). They added to MSC cultures. Real-time quantitative reverse transcription polymerase chain reactions blot...
Abstract Cellular senescence plays an important role in different biological and pathological conditions. Senescent cells communicate with their microenvironment through a plethora of soluble factors, metalloproteases extracellular vesicles (EV). Although much is known about the that factors play senescence, downstream signalling pathways activated by EV unknown. To address this, we performed small molecule inhibitor screen have identified IκB kinases IKKε, IKKα IKKβ as essential for...
Mesenchymal stem cells have an important potential in the treatment of age-related diseases. In last years, small extracellular vesicles derived from these been proposed as cell-free therapies. Cellular senescence and proinflammatory activation are involved loss therapeutic capacity phenomenon called inflamm-aging. The regulators two biological processes mesenchymal not well-known. this study, we found that p65 is activated during cellular inflammatory human umbilical cord-derived cell. To...
Epithelial ovarian cancer (EOC) is the most lethal gynecological cancer. The current treatment for EOC involves surgical debulking of tumors followed by a combination chemotherapy. While patients achieve complete remission, many EOCs will recur and develop chemo-resistance. cells can adapt to several stress stimuli, becoming resistant. Because this, new ways fight resistant during disease are being studied. However, clinical outcomes remain unsatisfactory. Recently, ferroptosis, novel form...
Nuclear accumulation of a mutant form the nuclear protein Lamin-A, called Progerin (PG) or Lamin AΔ50, occurs in Hutchinson-Gilford Progeria Syndrome (HGPS) Progeria, an accelerated aging disease. One main symptoms this genetic disorder is loss sub-cutaneous fat due to dramatic lipodystrophy.We stably induced expression human PG and GFP -Green Fluorescent Protein- as control 3T3L1 cells using lentiviral system study effect differentiation capacity cell line, one most used adipogenic models....
Abstract During this last decade, the development of prosenescence therapies has become an attractive strategy as cellular senescence acts a barrier against tumour progression. In context, CDK4/6 inhibitors induce and reduce growth in breast cancer patients. However, even though cells are arrested after inhibitor treatment, genes regulating context still unknown limiting their antitumour activity. Here, using functional genome-wide CRISPR/Cas9 genetic screen we found several that participate...
Osteoarthritis (OA) is closely linked to the increase in number of senescent cells joint tissues, and senescence-associated secretory phenotype (SASP) implicated cartilage degradation. In last decade, extracellular vesicles (EV) combination with use miRNAs modify post-transcriptional expressions multiple genes have shown their utility new therapies treat inflammatory diseases. This work delves into anti-inflammatory effect derived from mesenchymal stem (MSC) previously modified inhibit...
Senescence is a process characterized by prolonged irreversible cell-cycle arrest. The accumulation of senescent cells in tissues related to aging and the development age-related diseases. Recently, gene therapy has emerged as powerful tool for treating age-associated diseases transference specific genes into target cell population. However, high sensitivity significantly precludes their genetic modification via classical viral non-viral systems. Niosomes are self-assembled nanocarriers that...
Hutchinson-Gilford progeria syndrome (HGPS) is a very rare fatal disease characterized for accelerated aging. Although the causal agent, point mutation in LMNA gene, was identified more than decade ago, molecular mechanisms underlying HGPS are still not fully understood and, currently, there no cure patients, which die at mean age of thirteen. With aim unraveling non-previously altered pathways premature aging process, human cell lines from patients and healthy parental controls were studied...
Xenogeneic chondrocytes and allogeneic mesenchymal stem cells (MSC) are considered a potential source of for articular cartilage repair. We here assessed the immune response triggered by xenogeneic when injected intraarticularly, as well immunoregulatory effect bone marrow-derived MSC after systemic administration. To this end, discordant xenotransplantation model was established injecting three million porcine (PAC) into femorotibial joint Lewis rats monitoring response. First, fate using...
Our group focuses on the study of mesenchymal stem cells (MSCs) from human umbilical cord stroma or Warthońs jelly and their directed differentiation toward chondrocyte-like capable regenerating damaged cartilage when transplanted into an injured joint. This aimed to determine whether lactogenic hormone prolactin (PRL) 3, 3', 5-triiodo-L-thyronine (T3), active thyroid hormone, modulates chondrogenesis in our vitro model chondrogenic differentiation, Wnt signalling is involved this...
Hutchinson–Gilford progeria syndrome (HGPS) is a deadly childhood disorder, which considered very rare disease. It caused by an autosomal dominant mutation on the LMNA gene, and it characterized accelerated aging. Human cell lines from HGPS patients healthy parental controls were studied in parallel using next-generation sequencing (NGS) to unravel new non-previously altered molecular pathways. Nine hundred eleven transcripts differentially expressed when comparing versus total of 21,872...
Small extracellular vesicles released by fibroblasts from young human donors diminish lipid peroxidation in senescent cells and different old mice organs due to their enrichment Glutathione-S-transferase Mu antioxidant activity.