A5067184965- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Eicosanoids and Hypertension Pharmacology
- Cancer, Hypoxia, and Metabolism
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Fatty Acid Research and Health
- Diet and metabolism studies
- Analytical chemistry methods development
- Sulfur Compounds in Biology
- Lipid metabolism and biosynthesis
- Food Quality and Safety Studies
- Reproductive Biology and Fertility
- Pesticide Residue Analysis and Safety
- Advanced Proteomics Techniques and Applications
- Cancer, Lipids, and Metabolism
- Alcohol Consumption and Health Effects
- Viral Infections and Vectors
- Maternal Mental Health During Pregnancy and Postpartum
- Enzyme Catalysis and Immobilization
- Pharmacological Effects and Toxicity Studies
- Ovarian function and disorders
Wuhan Textile University
2023-2025
Wuhan University
2015-2024
Macau University of Science and Technology
2022
Hubei University
2013-2014
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class lipid mediators with promising anti-diabetic and anti-inflammatory properties. Comprehensive screening identification FAHFAs in biological samples would be beneficial to the discovery enable greater understanding their functions. Here, we report comprehensive rice Arabidopsis thaliana by chemical isotope labeling-assisted liquid chromatography–mass spectrometry (CIL-LC–MS). Multiple reaction monitoring (MRM) was used for...
Gut microbiota plays important roles in the host health. The and symbiotic gut coproduce a large number of metabolites during metabolism food xenobiotics. analysis fecal can provide noninvasive manner to study outcome host-gut interaction. Herein, we reported comprehensive profiling metabolome mice by an integrated chemical isotope labeling combined with liquid chromatography-mass spectrometry (CIL-LC-MS) analysis. are categorized into several submetabolomes based on functional moieties...
Flow injection mass spectrometry (FI-MS) is widely employed for high-throughput metabolome analysis, yet the absence of prior separation leads to significant matrix effects, thereby limiting coverage. In this study, we introduce a novel photosensitive MS probe, iTASO-ONH2, integrated with FI-MS establish strategy submetabolome analyses. The iTASO probe features conjugated-imino sulfonate moiety efficient photolysis under 365 nm irradiation and reactive group selective metabolite labeling....
Chemical labeling (CL) in combination with liquid chromatography-mass spectrometry (LC-MS) analysis has been demonstrated to be a promising technology metabolomic analysis. However, identification of chemically labeled metabolites remains challenging. Retention time (RT) is one the most important parameters for metabolites, but it could vary greatly LC-MS In this work, we developed chemical labeling-based HPLC retention index (CL-HPLC RI) strategy facilitate metabolites. CL-HPLC RI strategy,...
Branched fatty acid ester of hydroxy (FAHFA) is a class natural lipid with important biological functions. In this study, we first profiled natural-origin FAHFAs in different teas using the chemical labeling-assisted liquid chromatography-mass spectrometry method. Consequently, observed rich molecular diversity multiple regioisomers teas. Additionally, FAHFA contents had positive relationship tea fermentation degree and negative homologous acids. Moreover, highly accumulated (e.g., 3-MAHMA)...
The gut microbiota may affect mood through the microbiota-gut-brain axis. purpose of this study was to examine effect and its metabolites, such as short-chain fatty acids (SCFAs), on prenatal depression determine role 5-hydroxytryptamine (5-HT) in association with metabolites (i.e. SCFAs). Eighty-six pregnant women third trimester were recruited. Prenatal determined by a score 10 via Edinburgh Postpartum Depression Scale. Demographic data, stool, blood samples collected. SCFAs 16S rRNA gene...
Hydroxymethanesulfonate (HMS), the product of HSO3– and SO32– reacting with dissolved formaldehyde in fog, cloud, aerosol liquid water, has been suggested to be an important source particulate sulfur during haze events. However, there have limited studies high concentrations HMS atmospheric aerosol. We report here two feasible analytical methods for identification using ion chromatography UHPLC-LTQ-Orbitrap mass spectrometry. The fine particles wintertime Beijing ranged from below detection...
Although chemoselective labeling strategies show great potential in in-depth description of metabolomics, the associated time and expense limit applications high-throughput routine analysis. We report a fast effective strategy based on multifunctionalized monolithic probes. A rapid pH-responsive boronate ester reaction was employed to immobilize release probe molecules from substrate 5 min. The mesoporous surface hierarchically porous channels allowed for accelerated reactions. Moreover,...
Gut microbiota–host co-metabolites serve as essential mediators of communication between the host and gut microbiota. They provide nutrient sources for cells regulate microenvironment, which are associated with a variety diseases. Analysis is great significance to explore host–gut microbiota interaction. In this study, we integrated chemical derivatization, liquid chromatography–mass spectrometry, molecular networking (MN) establish novel CD-MN strategy analysis carboxylated metabolites in...
In vitro fertilization (IVF) is a highly effective treatment for infertility; however, it poses challenges women with decreased ovarian reserve (DOR). Despite the importance of understanding impact DOR on IVF outcomes, limited research has explored this relationship, particularly using omics approaches. Hence, we conducted study to investigate association between and employing metabolomic approach. We analyzed serum samples from 207 undergoing treatment, including 89 118 normal (NOR). Our...
Hydrophilic interaction liquid chromatography–mass spectrometry (HILIC-MS) is a complementary technique to reversed-phase (RPLC-MS) and has been widely used expand the coverage of metabolome in MS-based metabolomics. However, use HILIC retention time (HILIC RT) metabolites annotation quite limited because its poor reproducibility. Here, we developed method calculate index RI) for calibration RT. In this method, mixture 2-dimethylaminoethylamine (DMED)-labeled fatty acid standards with carbon...
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a family recently discovered lipids with important physiological functions in mammals and plants. However, low detection sensitivity negative ionization mode mass spectrometry makes low-abundance FAHFA challenging to analyze. A 2-dimethylaminoethylamine (DMED) based chemical derivatization strategy was reported improve the MS FAHFAs by labeling positively ionizable tertiary amine group. To facilitate reliable, high-throughput, automatic...
Bile acids (BAs) are a type of gut microbiota–host cometabolites with abundant structural diversity, and they play critical roles in maintaining host–microbiota homeostasis. In this study, we developed new N-(4-aminomethylphenyl) pyridinium (AMPP) derivatization-assisted alternating dual-collision energy scanning mass spectrometry (AMPP-dual-CE MS) method for the profiling BAs derived from host–gut microbiota cometabolism mice. Using proposed method, discovered two types amino acid...
Severe fever with thrombocytopenia syndrome (SFTS) is a viral infection primarily found in Asia, case fatality rate of about 10%. Despite its increasing prevalence, the underlying pathogenic mechanisms remain poorly understood, limiting development effective therapeutic interventions. We employed an untargeted metabolomics approach using liquid chromatography-mass spectrometry (LC-MS) to analyze serum samples from 78 SFTS patients during acute phase their illness. Differential metabolic...
Background/Objectives: Prenatal depression (PND) poses a significant threat to the health of both mother and developing fetus. Despite its increasing prevalence, pathophysiology PND is not yet fully elucidated. Methods: In this study, we aimed investigate fecal metabolites gut microbiota in patients compared healthy controls explore potential correlations between these factors. Results: Through untargeted metabolomics analysis, identified 75 significantly altered patients, which 27 were...