A5100379555- Advanced Photocatalysis Techniques
- Microbial Metabolic Engineering and Bioproduction
- Vitamin D Research Studies
- Gas Sensing Nanomaterials and Sensors
- Cyclopropane Reaction Mechanisms
- Vitamin C and Antioxidants Research
- Cardiac Ischemia and Reperfusion
- Copper-based nanomaterials and applications
- Bone health and osteoporosis research
- Catalytic C–H Functionalization Methods
- Enzyme Catalysis and Immobilization
- Monoclonal and Polyclonal Antibodies Research
- Dermatology and Skin Diseases
- Catalytic Cross-Coupling Reactions
- Molecular Sensors and Ion Detection
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Soft tissue tumors and treatment
- Alcohol Consumption and Health Effects
- Diet and metabolism studies
- Urticaria and Related Conditions
- MicroRNA in disease regulation
- Catalytic Alkyne Reactions
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
Liaocheng University
2024
Xi'an Jiaotong University
2024
Nanyang Normal University
2015-2023
Huaian First People’s Hospital
2023
Tianjin Children's Hospital
2022
Zhengzhou University
2017-2019
Harbin Medical University
2017
Second Affiliated Hospital of Harbin Medical University
2017
Sichuan University
2016
Anyang Normal University
2015
Nicotinamide riboside kinase (NRK) plays an important role in the synthesis of β -nicotinamide nucleotide (NMN). NMN is a key intermediate NAD + synthesis, and it actually contribute to well-being our health. In this study, gene mining technology was used clone nicotinamide nucleoside fragments from S. cerevisiae , Sc NRK1 achieved high level soluble expression E. coli BL21. Then, reScNRK1 immobilized by metal affinity label optimize enzyme performance. The results showed that activity...
Previous studies have suggested that vitamin D is associated with cardiovascular disease (CVD), however, the relationship between levels and CVD risk still unclear. The purpose of this study was to assess serum concentration 25-hydroxyvitamin (25(OH)D) in rural residents Henan province China. Basic information medical history were gathered through face-to-face surveys from July 2013 August 2015, biochemical indicators a laboratory setting. Logistic restricted cubic splines regression...
Vitamin D deficiency is implicated in some diseases, including cardiovascular. Few studies have assessed the correlation between 25-hydroxyvitamin D3 [25(OH) D3] and serum lipids. In this study, we explored 25(OH) concentrations lipids with a typical sample of rural population China.Face-to-face research was used to gather basic information. Fasting 25-(OH) D3, total cholesterol (TC), triglyceride (TG), HDL (HDL-C) and, LDL (LDL-C) tested laboratory.The mean level 28.71 ± 29.29 ng/mL. The...
Riboflavin (RF) and cysteamine (Cys) are vital in clinical other fields, but their detection is often intricate reliant on costly equipment materials. Plasmon-enhanced fluorescence (PEF) using plasmonic nanoparticles offers a solution to enhance the optical properties of fluorophores. Additionally, quenching becomes apparent when distance between fluorophores nanostructures shrinks few nanometers. Harnessing these effects holds tremendous promise for improved performance. In this study, PEF...
Acetaldehyde dehydrogenase (ALDH) is a common oxidoreductase and one of the aldehyde dehydrogenases, which can convert toxic acetaldehyde to harmless acetyl CoA. A novel (LpALDH) from Lactiplantibacillus plantarum was obtained by gene mining then analyzed series bioinformatics software. Phylogenetic tree results showed that LpALDH highly homologous with derived Pediococcus sp., their sequence similarity 62.9%. Moreover, it successfully expressed in Escherichia coli BL21, its expression level...
Adenylate kinase (AK) from Escherichia coli was used as both solubility and affinity tag for recombinant protein production. When fused to the N-terminus of a target protein, an AK fusion could be expressed in soluble form purified near homogeneity single step Blue-Sepherose via elution with micromolar concentration P1, P5- di (adenosine-5') pentaphosphate (Ap5A), transition-state substrate analog AK. Unlike any other tags, level expression its yield recovery during each purification readily...
Visible-light-induced palladium-catalyzed formal [3 + 3] cyclization of 2-iodophenols and cyclopropenes for the synthesis 2,2-disubstituted 2 H -chromenes with a quaternary carbon center in moderate to good yields.
In the pharmaceutical and chemical industry, L-o-chlorophenylglycine its derivatives are very important building blocks. Nevertheless, catalytic activity of natural D-mandelate dehydrogenase (DMDH) toward D-o-chloromandelic acid (D-o-CMA) was low, which greatly limits industrial application. Taking Lactobacillus harbinensis (LhDMDH) as parent, laboratory evolution performed to improve performance acid. An A59G/N252G mutant LhDMDH with a higher obtained, approximately 8.4 times than that...
摘要： 新生儿男，出生23 d，因出生即发现右肘部肿物于2021年2月就诊。患儿系第1胎，第1产，足月剖宫产。否认局部外伤史，既往史及家族史无特殊……