A5020526599- Dietetics, Nutrition, and Education
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Nutrition and Health in Aging
- Astro and Planetary Science
- Machine Learning in Bioinformatics
- CRISPR and Genetic Engineering
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Protein Structure and Dynamics
- Protein Kinase Regulation and GTPase Signaling
- Extracellular vesicles in disease
- Planetary Science and Exploration
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
- Neuropeptides and Animal Physiology
- Endoplasmic Reticulum Stress and Disease
- Bioinformatics and Genomic Networks
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Protein Hydrolysis and Bioactive Peptides
- Genomics and Phylogenetic Studies
University of Nevada, Las Vegas
2014-2024
Baylor Scott & White Health
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2002-2023
University of Copenhagen
2020-2023
Nevada Division of Public and Behavioral Health
2018-2023
University Hospital Heidelberg
2009-2020
Heidelberg University
2010-2020
National Center for Tumor Diseases
2015
Deutsches Diabetes-Zentrum e.V.
2013
Heinrich Heine University Düsseldorf
2013
To analyze clinical manifestations, serum ferritin, and cytokine levels in patients with adult-onset Still's disease (AOSD) or bacterial sepsis to evaluate their potential use for differential diagnosis.Twenty-two consecutive the first flare of AOSD 6 an established diagnosis under immunosuppressive therapy were compared 14 sepsis. Clinical manifestations scored a Pouchot activity score including elevated ferritin obtain modified score. Serum profiles analyzed from each patient.The scores...
Cellular functions require specific protein-protein interactions that are often mediated by modular domains use binding pockets to engage particular sequence motifs in their partners. Yet, how different members of a domain family select for distinct is not fully understood. The human genome encodes 120 Src homology 2 (SH2) (in 110 proteins), which mediate proteins with diverse phosphotyrosine (pTyr)-containing sequences. structure the SH2 BRDG1 bound peptide revealed pocket was blocked loop...
Extracellular vesicles (EVs) are released from nearly all mammalian cells and different EV populations have been described. Microvesicles represent large EVs (LEVs) the cellular surface, while exosomes small (SEVs) an intracellular compartment. As it is likely that stimuli promote release of distinct populations, we analyzed human lymphocytes considering respective (activation vs. apoptosis induction). We could clearly separate two namely SEVs (average diameter <200nm) LEVs (diameter range...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and fat bone marrow.In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, adipocyte crucial metabolism.While high cell-to-cell heterogeneity gene expression, cell subtypes contribute this vivo...
A decline in diagnostic testing for SARS-CoV-2 is expected to delay the tracking of COVID-19 variants concern and interest United States. We hypothesize that wastewater surveillance programs provide an effective alternative detecting emerging assessing incidence, particularly when clinical limited. Here, we analyzed RNA from eight locations across Southern Nevada between March 2020 April 2021. Trends concentrations (ranging 4.3 log
The silent clearance of apoptotic cells is essential for cellular homeostasis in multicellular organisms, and several mediators cell recognition have been identified. However, the distinct mechanisms involved are not fully deciphered yet. We analyzed alterations glycocalyx on surfaces its impact engulfment. After apoptosis induction lymphocytes, a decrease α2,6-terminal sialic acids α2,3-linkage with galactose was observed. Similar changes were to be found surface membrane blebs released...
Inappropriate clearance of apoptotic remnants is considered to be the primary cause systemic autoimmune diseases, like lupus erythematosus. Here we demonstrate that cells release distinct types subcellular membranous particles (scMP) derived from endoplasmic reticulum (ER) or plasma membrane. Both scMP exhibit desialylated glycotopes resulting surface exposure immature ER-derived glycoproteins surface-borne sialidase activity, respectively. Sialidase activity activated by caspase-dependent...
We present a series of papers dedicated to modelling the accretion and differentiation rocky planets that form by pebble within lifetime protoplanetary disc. In this first paper, we focus on how accreted ice determines distribution iron between mantle (oxidized FeO FeO$_{1.5}$) core (metallic Fe FeS). find an initial primitive composition ice-rich material leads, upon heating decay $^{26}$Al, extensive water flow formation clay minerals inside planetesimals. Metallic dissolves in liquid...
Astrocytic tumors occasionally arise in the central nervous system following radiotherapy. It is not clear if these gliomas represent a unique molecular genetic subset. We identified nine cases which an astrocytoma arose within ports of previous radiation therapy, with total doses ranging from 2400 to 5500 cGy. Irradiated primary lesions included craniopharyngioma, pituitary adenoma, Hodgkin's lymphoma, ependymoma, pineal neoplasm, rhabdomyosarcoma, and three lymphoblastic malignancies....
Lipid rafts and the formation of an immunological synapse are crucial for T‐cell activation. Binding cholera toxin B subunit (CTB) to ganglioside GM1 is a marker identify lipid rafts. Primary human T cells were isolated from healthy donors stimulated with superantigen staphylococcus enterotoxin (SEB) stained B‐fluorescein isothiocyanate (CTB‐FITC). An optimized staining procedure required stain exclusively on cell surface. Unstimulated show few CTB binding spots The size number CTB‐binding...
Minimotif Miner (MnM available at http://minimotifminer.org or http://mnm.engr.uconn.edu) is an online database for identifying new minimotifs in protein queries. Minimotifs are short contiguous peptide sequences that have a known function least one protein. Here we report the third release of MnM which has now grown 60-fold to approximately 300 000 minimotifs. Since by their nature not very complex also summarize set false-positive filters and linear regression scoring vastly enhance...
Phosphotyrosine (pTyr) signaling, which plays a central role in cell-cell and cell-environment interactions, has been considered to be an evolutionary innovation multicellular metazoans. However, neither the emergence nor evolution of human pTyr signaling system is currently understood. Tyrosine kinase (TK) circuits, each consists TK writer, substrate, related reader, such as Src homology (SH) 2 domains pTyr-binding (PTB) domains, comprise core machinery network. In this study, we analyzed...
Clinical studies failed to provide clear evidence for a proatherogenic role of hypercoagulability. This is in contrast the well-established detrimental hypercoagulability and thrombin during acute atherosclerotic complications. These seemingly opposing data suggest that might exert both antiatherogenic effects. We therefore investigated whether mediates beneficial effect de novo atherogenesis.De atherogenesis was evaluated 2 mouse models with hyperlipidemia genetically imposed...
The amino acid arginine is fundamentally involved in the regulation of immune response during infection, inflammatory diseases and tumor growth. Arginine deficiency (e.g. due to myeloid cell enzyme arginase) inhibits proliferation effector functions activated T lymphocytes. Here, we studied intracellular mechanisms mediating this suppression human Our proteomic analysis revealed an impaired dephosphorylation actin-binding protein cofilin upon T-cell activation absence arginine. We show that...
Membrane microparticles (MMP) released from apoptotic cells deliver signals that secure the anti-inflammatory response beyond nearest proximity of cell. Plasmacytoid dendritic (pDC) are sentinels prepared to detect cellular processes endanger organism. They play a key role in regulation both pro- and immune responses. Based on assumption pDC could participate initiation cells, we investigated effects cell-derived MMP human pDC. The results obtained our experiments confirmed trigger IFN-α...