A5100319654- Microbial Natural Products and Biosynthesis
- RNA Research and Splicing
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Amyotrophic Lateral Sclerosis Research
- Herpesvirus Infections and Treatments
- Electrochemical sensors and biosensors
- Plant Molecular Biology Research
- Cancer-related gene regulation
- Neurogenetic and Muscular Disorders Research
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Sensors
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Soil and Water Nutrient Dynamics
- Synthetic Organic Chemistry Methods
- Immune Cell Function and Interaction
- Gut microbiota and health
- Acute Ischemic Stroke Management
- Drug Transport and Resistance Mechanisms
- Soil Carbon and Nitrogen Dynamics
Shandong First Medical University
2018-2025
Nanjing Medical University
2012-2025
Peking University
2012-2025
National Health and Family Planning Commission
2019-2025
Academy of Medical Sciences
2023-2025
Bengbu Medical College
2020-2025
Xiangya Hospital Central South University
2022-2024
Central South University
2022-2024
Hunan Provincial Center for Disease Control and Prevention
2024
Suzhou Traditional Chinese Medicine Hospital
2024
To improve the quality control of drugs, we predicted absorption, distribution, metabolism, excretion, and toxicity (ADMET) ceftazidime (CAZ) its impurities via in silico methods. We used three types quantitative structure-activity relationship docking software for precise prediction: Discovery Studio 4.0, OECD QSAR Toolbox 4.1, Toxtree, pkCSM approach. The pharmacokinetics impurity A (Δ-2-CAZ) are similar. biological properties B (CAZ E-isomer) different from CAZ. Therefore, focused on drug...
Stress granules are dynamic, reversible condensates composed of RNA and protein that assemble in eukaryotic cells response to a variety stressors normally disassembled after stress is removed. The composition assembly well understood, but little known about the mechanisms govern disassembly. Impaired disassembly has been implicated some diseases including amyotrophic lateral sclerosis, frontotemporal dementia, multisystem proteinopathy. Using cultured human cells, we found granule was...
Stress granules (SGs) are non-membrane-bound RNA-protein that assemble through phase separation in response to cellular stress. Disturbances SG dynamics have been implicated as a primary driver of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), suggesting the hypothesis these diseases reflect an underlying disturbance material properties SGs. However, this concept has remained largely untestable available models assembly, which...
DL-3-n-butylphthalide (NBP) is a drug for treating acute ischemic stroke and may play neuroprotective role by acting on multiple active targets. The efficacy of NBP in patients with receiving reperfusion therapy remains unknown. To assess the safety intravenous thrombolysis and/or endovascular treatment. This multicenter, double-blind, placebo-controlled, parallel randomized clinical trial was conducted 59 centers China 90-day follow-up. Of 1236 stroke, 1216 18 years older diagnosed National...
Malignant gliomas are largely refractory to immune checkpoint blockade (ICB) therapy. To explore the underlying regulators, we examine microenvironment in glioma and find that tumor-infiltrating T cells mainly confined perivascular cuffs express high levels of CCR5, CXCR3, programmed cell death protein 1 (PD-1). Combined analysis clustering with receptor (TCR) clone expansion shows potential tumor-killing categorized into pre-exhausted/exhausted effector CD8+ subsets, as well cytotoxic CD4+...
G3BP1/2 are paralogous proteins that promote stress granule formation in response to cellular stresses, including viral infection. The nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inhibits assembly and interacts with via an ITFG motif, residue F17, the N protein. Prior studies examining impact G3PB1-N interaction on SARS-CoV-2 replication have produced inconsistent findings, role this pathogenesis is unknown. Here, we use structural biochemical...
In this letter, gate leakage mechanisms in different contact normally off p-GaN/AlGaN/GaN high electron mobility transistors (HEMTs) have been studied by the temperature-dependent current-voltage (IG-VG) measurement. It is found that two-dimensional variable range hopping responsible for current at reverse bias and low forward both high-leakage low-leakage Schottky devices. At bias, case of contact, dominant conduction mechanism to be thermionic field emission while it Poole–Frenkle (PFE)...
Severe neurological symptoms are associated with Coronavirus disease 2019 (COVID-19). However, the morphologic features, pathological nature and their potential mechanisms in patient brains have not been revealed despite evidence of neurotropic infection. In this study, neuropathological damages infiltrating inflammatory cells were quantitatively evaluated by immunohistochemical staining, ultrastructural examination under electron microscopy, an image threshold method, postmortem from nine...
Human metapneumovirus (HMPV) is one of the main pathogens causing severe respiratory infections in children, as a common cause immunodeficiency-related deaths children and elderly individuals, prevalence HMPV has been showing an increasing trend during last years. However, no vaccines or effective treatment plans are available currently. In this present, based on candidate proteins highly associated with viral virulence promising protective potential, we screened for immunodominant cytotoxic...
The benefits of improving coverage and timeliness varicella vaccination need to be quantified in countries where vaccine (VarV) has not yet been included national immunization programs. This longitudinal study analyzed the effectiveness (VE) program implemented Changzhou City during transitional period (2017-2022). Using Immunization Information System National Notifiable Infectious Disease Surveillance registry data, this retrospective case-cohort assessed VEs for children born from 2016...
Sample relatedness is a major confounder in genome-wide association studies (GWAS), potentially leading to inflated type I error rates if not appropriately controlled. A common strategy incorporate random effect related genetic matrix (GRM) into regression models. However, this approach challenging for large-scale GWAS of complex traits, such as longitudinal traits. Here we propose scalable and accurate analysis framework, SPAGRM, which controls sample via precise approximation the joint...
Ischemic stroke causes blood–brain barrier (BBB) breakdown due to significant damage the integrity of BBB components. Recent studies have highlighted importance pericytes in repair process functions triggered by PDGFRβ up-regulation. Here, we show that perlecan, a major heparan sulfate proteoglycan basement membranes, aids maintenance and through pericyte interactions. Using transient middle cerebral artery occlusion model, found larger infarct volumes more leakage conditional perlecan...
Abstract Background The prevalence of carbapenem-resistant Klebsiella pneumoniae bloodstream infection (CRKP-BSI) is increasing worldwide. CRKP-BSI associated with high rates morbidity and mortality due to limited antibiotic choices. Here, we aim identify the risk factors for CRKP BSI. Methods This was a retrospective study past data from January 1st, 2012 December 31st, 2019 adult patients KP-BSI in Xiangya Hospital, China. Results Among 706 incidences included this study, 27.4% them...
Root exudation fulfills fundamental roles in regulating carbon (C)-nutrient cycling forest ecosystems, yet the main ecological drivers of root and underlying mechanisms forests under natural gradients remain poorly understood. Here, we investigated intraspecific variation rates two alpine coniferous (Abies faxoniana Rehder et Wilson Abies georgei Orr) along elevation on eastern Tibetan Plateau. Meanwhile, fine traits associated climate soil parameters were assessed to examine effects...