A5005516079- Diphtheria, Corynebacterium, and Tetanus
- Mycobacterium research and diagnosis
- Veterinary medicine and infectious diseases
- Parasitic Infections and Diagnostics
- Tuberculosis Research and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Toxoplasma gondii Research Studies
- Research on Leishmaniasis Studies
- Reproductive tract infections research
- Coccidia and coccidiosis research
- Herpesvirus Infections and Treatments
- Bee Products Chemical Analysis
- Essential Oils and Antimicrobial Activity
- Insects and Parasite Interactions
- Immunodeficiency and Autoimmune Disorders
- Seaweed-derived Bioactive Compounds
- Parasites and Host Interactions
- Trypanosoma species research and implications
- Reproductive System and Pregnancy
- vaccines and immunoinformatics approaches
- Insect and Pesticide Research
- Immune responses and vaccinations
- Phytochemistry Medicinal Plant Applications
- Parasite Biology and Host Interactions
- Synthesis and biological activity
Universidade Federal de Pelotas
2016-2025
National Council for Scientific and Technological Development
2017
Universidade Tiradentes
2014
Universidade Federal do Rio Grande
2013
Natural products continue to be an invaluable resource of anticancer drug discovery in recent years. Propolis is known for its biological activities such as antimicrobial and antitumor effects. This study assessed the effects Brazilian red propolis (BRP) on apoptosis migration potential human bladder cancer cells. The effect BRP ethanolic extract (25, 50, 100 μg/mL) 5637 cells was determined by MTT, LIVE/DEAD, (scratch assay) assays. Apoptosis induction investigated through flow cytometry...
The seroprevalence of Toxocara canis antibodies in children aged from 1 to 12 yr old was evaluated Pelotas City, Rio Grande do Sul, Brazil. Human toxocariasis or visceral larva migrans (VLM) diagnosed with the use an ELISA based on T. excretory–secretory (TES) antigens; Western blotting used confirm ELISA-positive results. From 427 samples, 50.6% were positive for presence anti-TES antibodies. A confirmatory test (Western blot) carried out a sample sera (n = 70), and all positive. blots had...
Most studies of Brazilian red propolis have explored the composition and biological properties its ethanolic extracts. In this work, we chemically extracted characterized essential oil (EOP) assessed adjuvant, antiparasitic cytotoxic activities. The chemical EOP was analyzed using gas chromatography with mass spectrometry (GC-MS). tested for in vitro activity against Trichomonas vaginalis (ATCC 30236 isolate); trophozoites were treated different concentrations (ranging from 25 to 500 μg/mL)...
SUMMARY Trichomonas vaginalis infections have been associated with other diseases so that epidemiological studies of the parasite are important and help to prevent spread disease. This study aimed determine prevalence T. in female patients 19 counties southwestern Rio Grande do Sul, Brazil. For diagnosis, was used direct examination, followed by applying a socio-epidemiological questionnaire. We analyzed 300 women 9% were infected vaginalis. The highest frequency occurred between 18 39 years...
Trichomoniasis is a parasitic infection caused by Trichomonas vaginalis and it considered to be the most common non-viral sexually transmitted in world. Since 1960s, nitroimidazoles such as metronidazole are drugs of choice for treatment trichomoniasis, but many adverse effects allergic reactions may result from their use. Reports metronidazole-resistant infections also highlight importance search new anti-T. agents. Considering this, herein we report evaluation 21 synthetic monocarbonyl...
Caseous lymphadenitis (CLA) is an infectious disease that affects small ruminants and caused by Corynebacterium pseudotuberculosis. This responsible for high economic losses due to condemnation trim of infected carcasses, decreased leather wool yield, loss sales breeding stock deaths from internal involvement. Treatment costly ineffective; the most cost-effective strategy timely immunisation. Various vaccine strategies have been tested, recombinant vaccines are a promising alternative. Thus,...
Caseous lymphadenitis (CLA) is a disease caused by Corynebacterium pseudotuberculosis. It affects mainly small ruminants and causes significant economic losses worldwide. Because symptoms are not immediately noticeable, CLA clinical diagnosis effective. Numerous serological tests being developed to detect the in asymptomatic animals, but currently available immunoassays have problems with sensitivity. Current ELISA formats use native bacterial antigens, recombinant proteins could be useful...
Toxocariasis is a neglected disease, and its main etiological agent the nematode Toxocara canis. Serological diagnosis performed by an enzyme-linked immunosorbent assay using T. canis excretory secretory (TES) antigens produced in vitro cultivation of larvae. Identification TES proteins can be useful for development new diagnostic strategies since few components have been described so far. Herein, we report results obtained proteomic analysis liquid chromatography-tandem mass spectrometry...
Caseous lymphadenitis is a chronic contagious disease that causes economic losses worldwide. Treatments are ineffective, thus demonstrating the importance of vaccination. In this study, rNanH and rPknG proteins from Corynebacterium pseudotuberculosis were associated with saponin or aluminum hydroxide adjuvants. Three experimental groups (10 animals each) immunized sterile 0.9% saline solution (G1), + Saponin (G2), Al(OH)3 (G3). The mice received two vaccine doses 21 days apart. Animals...
Chagas’ disease (CD) is an infectious attacking estimated 8 million people, mainly in rural areas of Latin America countries. CD has no effective treatment, evidencing the vaccination schedule as best control strategy. Although some medicaments are available, none them provides a solution for infection nor capable inducing protection. They also have questionable safety levels and side effects. In light this, several experimental vaccines development order to improve safety, reproducibility,...
<title>Abstract</title> Recently, new immunomodulatory compounds have benn sought as effective adjuvants in vaccine development. In this context, the bioactive substances from macroalgae stand out, they can satisfactorily activate an immune response against infectious diseases, such caseous lymphadenitis (CLA), caused by <italic>Corynebacterium pseudotuberculosis</italic>. This study aimed to evaluate adjuvant activity of <italic>Iridea cordata </italic>and <italic>Sacorpletis...
The increasing resistance of Trichomonas vaginalis to the only approved chemical family drugs for treatment, 5-nitroimidazoles, has prompted exploration new therapeutic agents against this prevalent non-viral sexually transmitted infection. Natural products have emerged as a significant source novel treatments trichomoniasis. aim study was evaluate anti-T. activity citral (3,7-dimethyl-2,6-octadienal), main constituent essential oil Cymbopogon species, commonly known lemongrass. Our findings...
Due to the growing population of pets, especially homeless dogs and cats, zoonoses still represent a significant public health problem. Toxoplasma gondii Toxocara spp. are epidemiologically important zoonotic agents as they etiological factors human toxoplasmosis toxocariasis, respectively. These parasites remain neglected even though substantially prevalent in rural areas. The aim this study was investigate T. canis seroprevalence risk seropositivity Pelotas municipality, Brazil....
Caseous lymphadenitis (CLA) is a small ruminant disease characterized by the development of granulomatous lesions in superficial and internal lymph nodes, as well some organs, causes significant economic losses worldwide. The aetiological agent CLA bacterium Corynebacterium pseudotuberculosis; however, commercially available diagnostic tools present problems with regard to specificity, which can lead false-negative results. This study aimed develop an indirect enzyme-linked immunosorbent...