A5101763809- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Plant Virus Research Studies
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Cancer Immunotherapy and Biomarkers
- Inflammatory Biomarkers in Disease Prognosis
- Muscle and Compartmental Disorders
- Herpesvirus Infections and Treatments
- Bioinformatics and Genomic Networks
- Gene Regulatory Network Analysis
- Immune cells in cancer
- Acute Kidney Injury Research
- Influenza Virus Research Studies
- Islamic Finance and Banking Studies
- interferon and immune responses
- Leech Biology and Applications
- Cytomegalovirus and herpesvirus research
- Plant Genetic and Mutation Studies
Chinese PLA General Hospital
2021-2025
St. Jude Children's Research Hospital
2023-2025
Xuzhou Central Hospital
2025
Xuzhou Medical College
2024-2025
ShanghaiTech University
2018-2025
Beijing University of Chinese Medicine
2024
Air Force Medical University
2021-2022
Xi'an Medical University
2022
Affiliated Hospital of North Sichuan Medical College
2021
North Sichuan Medical University
2021
EK1 is a broad-spectrum human coronavirus fusion inhibitor for combating infection of current and emerging coronaviruses.
SARS-CoV-2 causing the worldwide pandemic has changed people's life in multiple aspects dramatically since it's first identified Wuhan, China at end of 2019. While numbers infected patients and death toll keep vigorous increasing, curbing progression is an urgent goal. Efforts have been made to search for prophylactic therapeutic approaches including neutralizing antibodies development. By reviewing dozens studies on anti-spike identification, we concluded that (1) promising are being fished...
Using an event study approach, this paper investigates the impact of coronavirus pandemic (COVID-19) on consumer industry in Chinese stock market. We find that stocks belonging to was adversely impacted by first three trading days after incident. However, is transitory. With implementation government policies, returns maintain steady growth.
The study aimed to assess the value of pretreatment peripheral blood neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte (PLR) and systemic immune-inflammation index/albumin (SII/ALB) for predicting immunotherapy prognosis efficacy in Non-small cell lung carcinoma (NSCLC) treated with Immune checkpoint inhibitors (ICIs) opioids. A total 78 NSCLC patients received ICIs opioids were retrospectively collected. optimal cut-off values determined by receiver operating characteristic curves....
The majority of neutralizing antibodies (NAbs) against SARS-CoV-2 recognize the receptor-binding domain (RBD) spike (S) protein. As an escaping strategy, RBD virus is highly variable, evolving mutations to thwart a natural immune response or vaccination. Targeting non-RBD regions S protein thus provides viable alternative generating potential, robust NAbs. Using pre-pandemic combinatorial antibody library 1011, through alternate negative and positive screening 11 non-RBD-targeting are...
<title>Abstract</title> Cross-reactive antibodies that bind to diverse epitopes have been identified in Sarbecoviruses, but the precise molecular mechanisms remain poorly understood. This study describes isolation of a broadly neutralizing monoclonal antibody, 3D1, derived from fully human combinatorial antibody library targets highly conserved HR1 domain Sarbecoviruses. 3D1 uniquely recognizes type 1 β-turn fold consisting 6-mer epitopic peptide, pepDVVNQN/Q, which forms during pre-hairpin...
Human coronavirus OC43 (HCoV-OC43) is usually associated with common colds, but also related to severe disease in the frail. Its envelope glycoproteins spike (S) responsible for host-cell attachment and membrane fusion. To understand molecular basis of fusion HCoV-OC43, we solved 3.34 Å crystal structure post-fusion state formed by two heptad repeat domains (HR1P HR2P) OC43-S. This core comprises a parallel trimeric coiled coil three HR1 helices 61 at length, around which HR2 are entwined an...
Abstract Single-cell transcriptomics data present challenges due to their inherent stochasticity and sparsity, complicating both cell clustering type-specific network inference. To address these challenges, we introduce scMINER (single-cell Mutual Information-based Network Engineering Ranger), an integrative framework for unsupervised clustering, transcription factor signaling protein inference, identification of hidden drivers from single-cell transcriptomic data. demonstrates superior...
The use of medication in the neonatal intensive care unit (NICU) is a complex field that requires special attention, as patients may have different sensitivities and responses to drugs than adults older children. administration NICU must consider various factors, including dosage medication, route administration, monitoring, potential drug interactions. In this study, we conducted retrospective analysis 122 preterm infants treated our hospital from 2020 2023. Correlation revealed among...
Abstract Receptors and their ligands are important therapeutic targets for about one third of marketed drugs. Here, we describe an epitope-guided approach selection antibodies that modulate cellular signaling targeted receptors. We chose CXC chemokine receptor 2 (CXCR2) in the G-protein coupled superfamily as a CXCR2 N-terminal peptide antibody selection. obtain highly selective, tight-binding from 10 11 -member library using combinatorial enrichment. Structural Hydrogen-Deuterium-Exchange...
Human coronavirus 229E (HCoV-229E) usually causes mild upper respiratory infections in heathy adults, but may lead to severe complications or mortality individuals with weakened immune systems. Virus entry of HCoV-229E is mediated by its spike (S) protein, where the S1 domain facilitates attachment host cells and S2 involved subsequent fusion virus membranes. During process, two heptad repeats, HR1 HR2, assemble into a six-helix membrane-fusion structure termed core. Here, complete...
Objective This study evaluated the effect of systemic immune-inflammation index/albumin ratio (SII/ALB) on prognosis immunotherapy-treated patients receiving opioids. Methods A retrospective analysis was conducted 185 who received opioids at Xuzhou Central Hospital from 01/09/2021 to 01/09/2023. The results related clinical data were collected during week before cancer immunotherapy. SII/ALB cut-off value determined, and relationship between pathological parameters analyzed using chi-square...
The sparse nature of single-cell omics data makes it challenging to dissect the wiring and rewiring transcriptional signaling drivers that regulate cellular states. Many drivers, referred as "hidden drivers", are difficult identify via conventional expression analysis due low inconsistency between RNA protein activity caused by post-translational other modifications. To address this issue, we developed scMINER, a mutual information (MI)-based computational framework for unsupervised...
Abstract Potato tubers exhibiting necrotic rings/arcs were found in a winter potato crop ‘Favorita’ the subtropical area Huidong county, Guangdong province, China, 2012. When symptomatic cut crosswise, light to dark brown arcs observed tuber flesh. A mini-survey of 8% an 10 meters2. To reveal causal agent disease, reverse transcription–polymerase chain reactions (RT-PCR) targeting 9 common viruses including those (e.g, mop-top virus (PMTV), Tobacco rattle virus, Tomato spotted wilt and Y...
From the end of 2019, an ongoing outbreak a new type unexplained pneumonia caused by novel coronavirus, Severe Acute Respiratory Syndrome coronavirus 2 (SARS‑CoV‑2), was first identified in Wuhan, China. Since then, it has spread to most parts China and worldwide, thus affecting health individuals worldwide. Until August 2020, >25 million cases SARS‑CoV‑2 infection had been confirmed causing >800,000 deaths. This disease named World Health Organization as 2019 (COVID‑19). Similar SARS Middle...
Cross-species spillover to humans of coronaviruses (CoVs) from wildlife animal reservoirs poses marked and global threats human health. Recently, sporadic infection canine coronavirus–human pneumonia-2018 (CCoV-HuPn-2018) in hospitalized patients with pneumonia genetically related feline coronavirus were identified. In addition, swine acute diarrhea syndrome (SADS-CoV) had the capability broad tropism cultured cells including humans. Together, transmission Alphacoronaviruses that originated...
The complete genome comprising three genomic RNAs of Canadian and two Chinese isolates Potato mop-top virus were sequenced analysed. Two open reading frames (ORFs) found in RNA1 6.1 kb, encoding a readthrough RNA-dependent RNA polymerase (RdRp). A coat protein (CP)-readthrough was encoded by RNA2 3.1 kb. Four ORFs that the triple gene block proteins (TGBps) cysteine-rich RNA3 (2.9 kb) isolate 'Yunnan'; whereas remaining (three 'Guangdong'), only TGBps observed RNA3. single nucleotide...
In this study, a new SYBR Green qPCR (qRT-PCR) and nested RT-PCR (nRT-PCR) were developed to detect Potato mop-top virus (PMTV) in potato tuber tissues. The qRT-PCR nRT-PCR assays approximately 104- 103- fold more sensitive than the conventional assay. progeny tubers derived from PMTV-infected tested by RT-PCR, assays. Of 17 samples, 9 (52.9%) positive for PMTV 11 (64.7%) nRT-PCR, (100%) qRT-PCR. Compared was showed be sensitive. plants exhibited foliar symptoms including chlorosis reduction...
A preliminary screening for garlic viruses in plants Hunan, China, using existing monoplex (simplex) reverse transcription-polymerase chain reaction (RT-PCR) procedures detected four viruses/virus groups. These groups were Onion yellow dwarf virus (OYDV), Leek stripe (LYSV), Shallot latent (SLV) and allexiviruses (e.g., A, B, C, D, E, X). Sequence analysis of the projected allexivirus amplicons revealed infected was Garlic D (GarV-D), which shared 92–97% sequence identities with various...
In this paper, we report a case of coronavirus disease 2019 (COVID-19) complicated with human immunodeficiency virus (HIV) infection. The 50-year-old male patient had unexplained "intermittent fever sore throat" on February 9th, 2020. Chest computed tomography (CT) showed bilateral multiple patchy opacities and ground-glass opacities. A COVID-19 RNA test was positive. After admission, additional laboratory tests revealed the following: white blood cell (WBC) count, 4.7×109/L; neutrophil...
As a traditional Chinese medicine, leech has obvious pharmacological activities in anticoagulantion and antithrombosis.
Rotavirus (family Reoviridae) is the leading cause of severe gastroenteritis in human and animals worldwide. The genome rotavirus comprises 11 segments dsRNA encodes six structural proteins (VP1 to VP4, VP6 VP7) non (NSP1 NSP6). a group antigen (according disparation VP6, classified into seven groups: A G), major protein inner capsid particles (ICP), also specific mucosa immunization that mediate immunological reaction. In this report, sequence analysis gene giant panda was carried out....