A5082708983- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Nanoplatforms for cancer theranostics
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Photoacoustic and Ultrasonic Imaging
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- Inhalation and Respiratory Drug Delivery
- Lysosomal Storage Disorders Research
- Tissue Engineering and Regenerative Medicine
- Ultrasound and Hyperthermia Applications
- interferon and immune responses
- Neuroblastoma Research and Treatments
- Virus-based gene therapy research
- Cancer Research and Treatments
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Viral Infections and Outbreaks Research
- CAR-T cell therapy research
- Radiation Therapy and Dosimetry
- Ultrasound Imaging and Elastography
Vanderbilt University
2019-2024
Background Neuroblastoma (NB) is a childhood cancer for which new treatment options are needed. The success of immune checkpoint blockade in the adult solid tumors has prompted exploration immunotherapy NB; however, clinical evidence indicates that vast majority NB patients do not respond to single-agent inhibitors. This motivates need therapeutic strategies increase tumor immunogenicity. goal this study was evaluate immunotherapeutic strategy based on potent activation stimulator interferon...
PURPOSE We investigated quality and efficacy criteria of an autologous, physically immunologically purified, Newcastle disease virus (NDV)-modified, irradiated tumor-cell vaccine (ATV-NDV) by analyzing three independent cohorts (a through c) patients vaccinated between 1991 1995. MATERIALS AND METHODS Included were 63 with primary breast cancer (a), 27 metastatic pretreated (b), 31 ovarian (c). In addition to vaccine, b c received nonspecific immunotherapy as supportive treatment. After...
Radiation therapy (RT) is essential for triple negative breast cancer (TNBC) treatment. However, patients with TNBC continue to experience recurrence after RT. The role of the extracellular matrix (ECM) irradiated tissue in tumor still unknown. In this study, we evaluated structure, molecular composition, and mechanical properties murine mammary fat pads (MFPs) developed ECM hydrogels from decellularized tissues (dECM) assess effects RT-induced changes on cell behavior. Irradiated MFPs were...
Organoids derived from the digested tissue are multicellular three-dimensional (3D) constructs that better recapitulate in vivo conditions than cell monolayers. Although they cannot completely model complexity, retain some functionality of original organ. In cancer models, organoids commonly used to study tumor invasion. This protocol aims develop and characterize normal irradiated mouse mammary gland evaluate radiation response tissues. These can be applied future vitro studies interactions...
<ns4:p>The rising prevalence of high throughput screening and the general inability (1) two dimensional (2D) cell culture (2)<ns4:italic>in vitro</ns4:italic>release studies to predict<ns4:italic>in vivo</ns4:italic>neurobiological pharmacokinetic responses in humans has led greater interest more realistic three (3D) benchtop platforms. Advantages 3D human over its 2D analogue, or even animal models, include taking effects microgeometry long-range topological features into consideration. In...
Abstract Background While immunotherapy shows great promise in patients with triple negative breast cancer, many will not respond to treatment. Radiotherapy has the potential prime tumor-immune microenvironment for immunotherapy. However, predicting response is difficult due tumor heterogeneity across patients, which necessitates personalized medicine strategies that incorporate tracking into therapeutic approach. Here, we investigated use of ultrasound (US) imaging vasculature monitor...
While immunotherapy shows great promise in patients with triple negative breast cancer, many will not respond to treatment. Radiotherapy has the potential prime tumor-immune microenvironment for immunotherapy. However, predicting response is difficult due tumor heterogeneity across patients, which necessitates personalized medicine strategies that incorporate tracking into therapeutic approach. Here, we investigated use of ultrasound (US) imaging vasculature monitor
Organoids derived from the digested tissue are multicellular three-dimensional (3D) constructs that better recapitulate in vivo conditions than cell monolayers. Although they cannot completely model complexity, retain some functionality of original organ. In cancer models, organoids commonly used to study tumor invasion. This protocol aims develop and characterize normal irradiated mouse mammary gland evaluate radiation response tissues. These can be applied future vitro studies interactions...
<ns4:p>The rising prevalence of high throughput screening and the general inability (1) two dimensional (2D) cell culture (2)<ns4:italic>in vitro</ns4:italic>release studies to predict<ns4:italic>in vivo</ns4:italic>neurobiological pharmacokinetic responses in humans has led greater interest more realistic three (3D) benchtop platforms. Advantages 3D human over its 2D analogue, or even animal models, include taking effects microgeometry long-range topological features into consideration. In...
Abstract Tumor-associated macrophages (TAMs) represent more than 50% of the immune cells in breast tumors, even dysregulated, ‘cold’ tumors. TAMs that express an M2-like phenotype promote tumor growth and create immunosuppressive microenvironment (TME). High levels correlate with a poor prognosis. However, display phenotypic plasticity can be repolarized from one to another through signaling molecules, such as cytokines. Macrophages M1-like are inflammatory induce anti-tumor immunity....
Abstract Triple negative breast cancer (TNBC) recurrence rates remain high despite aggressive therapeutic intervention, including surgery, chemotherapy, and radiotherapy (RT). Recent studies suggest that circulating tumor cell re-seeding of primary tumors may facilitate rather than persistent cells in the irradiated surgical bed. However, role microenvironment is not well understood. An emerging risk factor for patients lymphopenia or abnormally low systemic lymphocyte counts following...
Triple negative breast cancer (TNBC) recurrence rates remain high despite aggressive therapeutic intervention, including surgery, chemotherapy, and radiotherapy (RT). Recent studies suggest that circulating tumor cell re-seeding of primary tumors may facilitate rather than persistent cells in the irradiated surgical bed. However, role microenvironment is not well understood. An emerging risk factor for patients lymphopenia or abnormally low systemic lymphocyte counts following therapy. In...
Abstract Radiation therapy (RT) is essential for triple negative breast cancer (TNBC) treatment. However, patients with TNBC continue to experience recurrence after RT. The role of the extracellular matrix (ECM) irradiated tissue in tumor still unknown. In this study, we evaluated structure, molecular composition, and mechanical properties murine mammary fat pads (MFPs) developed ECM hydrogels from decellularized tissues (dECM) assess effects RT-induced changes on cell behavior. Irradiated...
Abstract While most patients with triple negative breast cancer receive radiotherapy to improve outcomes, a significant subset of continue experience recurrence. Macrophage infiltration into radiation-damaged sites has been shown promote recurrence in pre-clinical models. However, the mechanisms that drive are unknown. Here, we developed novel spheroid model evaluate macrophage-mediated tumor cell recruitment. We first characterized infiltrating macrophage phenotypes irradiated mammary...