A5022460653- Osteoarthritis Treatment and Mechanisms
- Birth, Development, and Health
- Orthopedic Infections and Treatments
- Knee injuries and reconstruction techniques
- Pregnancy and preeclampsia studies
- Natural product bioactivities and synthesis
- Bone Metabolism and Diseases
- Adipose Tissue and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Total Knee Arthroplasty Outcomes
- Reproductive System and Pregnancy
- Orthopaedic implants and arthroplasty
- Proteoglycans and glycosaminoglycans research
- Inflammatory mediators and NSAID effects
- Fatty Acid Research and Health
- Surgical site infection prevention
- Chemokine receptors and signaling
- Bone and Joint Diseases
- Stress Responses and Cortisol
- Hip disorders and treatments
- Nanoplatforms for cancer theranostics
- Antimicrobial Resistance in Staphylococcus
- RNA Interference and Gene Delivery
- Gestational Diabetes Research and Management
- Neonatal Respiratory Health Research
Wuhan University
2015-2025
Zhongnan Hospital of Wuhan University
2015-2025
Hubei Provincial Center for Disease Control and Prevention
2018-2025
Wuhan Donghu University
2024
Wuhan Center for Disease Control and Prevention
2021-2022
Fourth Affiliated Hospital of Guangxi Medical University
2021
Abstract Osteoarthritis (OA) is significantly associated with diabetes, but how hyperglycemia induces or aggravates OA has not been shown. The synovium plays a critical role in cartilage metabolism and substance exchange. Herein, we intended to investigate whether affects the occurrence progression of by influencing synovium. In patients knee diabetes (DM OA), found more severe inflammatory response, higher endoplasmic reticulum stress (ERS) levels, advanced glycosylation end products (AGEs)...
Osteoarthritis (OA) is a degenerative joint disease characterized by low-grade inflammation and cartilage degradation. Dendrobine (DEN) reported to inhibit oxidative stress in some diseases, but its role chondrocyte senescence OA progress has not yet been elucidated. Our study aimed explore the protective effects of DEN on both vitro vivo. We found that inhibited extracellular matrix (ECM) degradation promoted ECM synthesis. Meanwhile, senescence-associated secretory phenotype (SASP) factors...
Prenatal dexamethasone exposure (PDE) induces developmental toxicities of multiple organs in offspring. Here, we verified the intergenerational effect low peak bone mass induced by PDE and investigated its intrauterine programming mechanism. Pregnant rats were injected subcutaneously with 0.2 mg/kg/d from gestation day (GD) 9 to 20. Some pregnant killed for fetuses on GD20, rest went spontaneous labor produce first-generation (F1) The adult F1 male offspring mated normal females F2 In vivo,...
Osteoporosis (OP) is a metabolic bone disease due to the imbalance of osteogenesis and resorption, in which, marrow mesenchymal stem cells (BMSCs) have significant effect as seed cells. Recent research has shown function Morusin on inhibiting osteoclast differentiation vitro. However, whether can regulate osteogenic addition proliferation BMSCs remains unclear. were isolated from 4-week-old Wistar rats then treated with different concentrations for 3, 5, 7, 14 days. The was detected by MTT...
Postmenopausal osteoporosis is associated with bone formation inhibition mediated by the impaired osteogenic differentiation potential of marrow mesenchymal stem cells (BMSCs). However, identifying and confirming essential genes in BMSCs remain challenging. The study aimed at revealing key gene that regulated led to osteoporosis, thus exploring its therapeutic effect osteoporosis. In present study, six related were identified, namely, fibrillin 2 (Fbn2), leucine-rich repeat-containing 17...
We previously showed that prenatal caffeine exposure (PCE) induces intrauterine growth retardation (IUGR) and high susceptibility to nonalcoholic fatty liver disease in offspring rats, the underlying mechanisms are associated with fetal overexposure maternal glucocorticoids. Herein, we aimed verify whether PCE disrupts development before after birth explore its possible programming mechanism. In vivo, reduced weights increased IUGR rates were accompanied by developmental dysfunction rats....
Osteoarthritis (OA) is a common degenerative disease that leads to pain, disability, and reduced quality of life. Orientin exhibits considerable anti-inflammatory antioxidative properties, but its role in chondrocyte senescence OA progress has not yet been fully characterized. The aim this study was evaluate the protective effects orientin on OA. extracellular matrix (ECM) degradation, mitochondrial homeostasis, investigated vitro. Meanwhile, we used molecular docking, small inhibitors, RNA...
Abstract Prenatal exposure to glucocorticoids is linked long‐term health risks in offspring, but the role of maternal gut microbiota mediating these effects remains unclear. Here, we demonstrate that prenatal prednisone therapy (PPT) humans and (PPE) rats result sex‐specific long bone dysplasia including reduced peak mass (PBM) heightened osteoporosis risk female offspring. Multi‐omics profiling fecal transplantation show PPE alters composition depletes microbial metabolite daidzein (DAI)....
Background Osteoarthritis (OA) is a chronic joints disease characterized by progressive degeneration of articular cartilage due to the loss matrix. Previously, we found, for first time, that an acidic glycan from Angelica Sinensis Polysaccharides (APSs), namely APS-3c, could protect rat OA promoting glycosaminoglycan (GAG) synthesis in chondrocytes. In present work, tried further understanding ASP-3c’s anti-OA activity. Methodology/Principal Findings Human primary chondrocytes were treated...
Steroid-induced osteonecrosis of the femoral head (SONFH) is a chronic and crippling bone disease. This study aims to reveal novel diagnostic biomarkers SONFH.The GSE123568 dataset based on peripheral blood samples from 10 healthy individuals 30 SONFH patients was used for weighted gene co-expression network analysis (WGCNA) differentially expressed genes (DEGs) screening. The in module related DEGs were extracted Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment...
The rate of eradication periprosthetic joint infection (PJI) caused by methicillin-resistant Staphylococcus aureus (MRSA) is still not satisfactory with systemic vancomycin administration after one-stage revision arthroplasty. This study aimed to explore the effectiveness and safety intraarticular (IA) injection in control MRSA PJI surgery a rat model. Two weeks intraperitoneal (IP) and/or IA was used surgery. rats treated showed better outcomes skin temperature, bacterial counts, biofilm on...
Treatment outcomes for methicillin-resistant Staphylococcus aureus (MRSA) periprosthetic joint infection (PJI) using systemic vancomycin and antibacterial cement spacers during two-stage revision arthroplasty remain unsatisfactory. This study explored the efficacy safety of intra-articular injections PJI control after debridement spacer implantation in a rat model.Total knee (TKA), MRSA inoculation, debridement, vancomycin-spacer were performed successively rats to mimic first-stage...
Prenatal nicotine exposure (PNE) induces developmental toxicity in offspring. However, the long‐term harmful effects on bone development and intrauterine programming mechanism attributed to PNE remain unclear. In present research, pregnant Wistar rats were injected subcutaneously with (2 mg/kg/d) obtain analyze samples from fetal adult Bone marrow mesenchymal stem cells (BMSCs) treated during osteogenic differentiation clarify related molecular mechanisms. The results indicated that led...
Abstract Background Aseptic necrosis of the femoral head (ANFH) has a high incidence in community and causes substantial problems with health as well economic social stress. Core decompression is most commonly used treatment for early ANFH. Although many studies have reported on efficacy core surgery ANFH, there are still some shortcomings assessing severity necrosis, location distribution, changes necrotic lesions before after surgery. Magnetic resonance imaging (MRI) equivalent sphere...
The objective of this study was to investigate the possible role UDP-glucose dehydrogenase (UGDH) in osteoarthritis (OA) and uncover whether, furthermore how interleukin-1beta (IL-1β) affects UGDH gene expression.UGDH specific siRNAs were applied determine proteoglycan (PG) synthesis human articular chondrocytes. Protein levels Sp1 rat OA cartilage detected. Then, primary chondrocytes treated with IL-1β find out whether could regulate expression its trans-regulators, that is Sp1, Sp3 c-Krox....
Although intra-articular vancomycin powder (VP) is sometimes applied before the closure of incision to prevent periprosthetic joint infection (PJI) after replacement, dosage, efficacy, and safety remain controversial. This study aimed explore VP in prophylaxis total knee arthroplasty (TKA) a rat model. Sixty male rats were randomly divided into five groups receiving TKA: control (no antibiotics); systemic (SV) (intraperitoneal injection, 88 mg/kg body weight, equal 1 g patient weighing 70...