A5059415863- Glycosylation and Glycoproteins Research
- Trace Elements in Health
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Advanced Proteomics Techniques and Applications
- Plant Micronutrient Interactions and Effects
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Chemical Reactions and Isotopes
- Advanced NMR Techniques and Applications
- Folate and B Vitamins Research
- Cancer-related gene regulation
- Fluorine in Organic Chemistry
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Potassium and Related Disorders
- RNA regulation and disease
- Selenium in Biological Systems
- Cannabis and Cannabinoid Research
- NMR spectroscopy and applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Methods in Pharmaceuticals
- Magnesium in Health and Disease
University of Massachusetts Amherst
2022-2024
University of Georgia
2019-2022
Los Alamos National Laboratory
2010
Creighton University
1969-1981
CHI Health Creighton University Medical Center - Bergan Mercy
1981
United States Department of Veterans Affairs
1975-1979
The enzyme UDP-glucose: glycoprotein glucosyltransferase (UGGT) is the gatekeeper of protein folding within endoplasmic reticulum (ER). One-third human proteome traverses ER where and maturation are facilitated by a complex homeostasis network. Both glycan modifications disulfide bonds key importance in these proteins. actions UGGT intimately linked to code for secretory proteins ER. selectively glucosylates N-linked misfolded so that they can reenter lectin-folding chaperone cycle be...
Zinc deficiency is associated with poor growth and development. It has been postulated that a of the various zinc metalloenzymes results from lack dietary zinc. From present study, an examination some aspects this postulate, we conclude following about in pigs: (1) Of tissues studied, it im diminished content only liver, kidney, pancreas. (2) tissue abnormalities trace elements other than (3) Histochemical enzyme assays are satisfactory for qualitative but not quantitative determination...
Abstract Methanogens and anaerobic methane-oxidizing archaea (ANME) are important players in the global carbon cycle. Methyl-coenzyme M reductase (MCR) is a key enzyme methane metabolism, catalyzing last step methanogenesis first oxidation. Divergent mcr -like genes have recently been identified uncultured archaeal lineages. However, assembly biochemistry of MCRs from remain largely unknown. Here we present an approach to study by heterologous expression methanogen, Methanococcus maripaludis...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-13 NMR spectroscopy of [20%-1,2-13C2-Gly6]-bradykinin. Role serine in reducing structural heterogeneityRobert E. London, John M. Stewart, Robert Williams, R. Cann, and N. A. MatwiyoffCite this: J. Am. Chem. Soc. 1979, 101, 9, 2455–2462Publication Date (Print):April 1, 1979Publication History Published online1 May 2002Published inissue 1 April...
The content of zinc, magnesium, calcium, bilirubin, and bile acids was determined in the hepatic zinc-deficient control swine. Zinc deficiency produced by dietary zinc restriction while pair fed supplemented animals served as controls for observations hepatobiliary functions. Pigs weighing 5 kg were placed two groups respective diets 6 weeks; hair skin abnormalities well decreased weight gain present group this time. Hepatic pancreatic juice obtained from each animal after careful isolation...
Zinc metabolism in cirrhotic patients was studied, indicating some improvement that did not alter the abnormalities of zinc significantly and showed no evidence correlation with clinical or lack thereof studied.
Preparation of samples for nuclear magnetic resonance (NMR) characterization larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N. This is routine that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates used. However, it expensive glycosylated mammalian more costly amino acids are usually We describe a simple, relatively inexpensive procedure which standard commercial media supplemented 13C-enriched...
Not all proteins are amenable to uniform isotopic labeling with 13C and 15N, something needed for the widely used, largely deductive, triple resonance assignment process. Among them expressed in mammalian cell culture where native glycosylation can be maintained, proper formation of disulfide bonds facilitated. Uniform cells is prohibitively expensive, but sparse one or a few isotopically enriched amino acid types an option these proteins. However, then relies on accessing best match between...
Abstract Human roundabout 1 (hRobo1) is an extracellular receptor glycoprotein that plays important roles in angiogenesis, organ development, and tumor progression. Interaction between hRobo1 heparan sulfate (HS) has been shown to be essential for its biological activity. To better understand the effect of HS binding we engineered a lanthanide-binding peptide sequence (Loop) into Ig2 domain hRobo1. Native mass spectrometry was used verify loop introduction did not inhibit or conformational...
N -glycosylation is a common posttranslational modification of secreted proteins in eukaryotes. This targets asparagine residues within the consensus sequence, N–X–S/T. While this sequence required for glycosylation, initial transfer high-mannose glycan by oligosaccharyl transferases A or B (OST-A OST-B) can lead to incomplete occupancy at given site. Factors that determine extent are not well understood, and understanding them may provide insight into function these important enzymes. Here,...
Characterization of glycosylated proteins and their response to interaction with glycans is challenging for any method, because the heterogeneity glycosylation extensive conformational sampling by most glycans. For NMR there are extra challenges expression in mammalian cell cultures, which can preserve near‐native glycosylation, requires expensive substrates achieve uniform isotopic labeling 15 N or 13 C, cells do not tolerate high levels deuteration (something required improve spectral...
Glycosylation is an important protein post-translational modification (PTM) that has been implicated in cancer, immune disorders, and other disease states. Analysis of this common PTM complicated by its heterogeneous nature, both the presence or absence a glycan structure attached itself. frequently studied either at level released glycans glycopeptides using mass spectrometry (MS), approach often termed “bottom-up”. A more technically demanding known as top-down (TDMS) involves analyzing...