A5028389694- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Botulinum Toxin and Related Neurological Disorders
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine regulation and behavior
- Retinal Development and Disorders
- Congenital heart defects research
- Hearing, Cochlea, Tinnitus, Genetics
- Biochemical and Structural Characterization
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Connexins and lens biology
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
- Genomics and Phylogenetic Studies
- Nerve injury and regeneration
- Endoplasmic Reticulum Stress and Disease
- Neurological disorders and treatments
- Photoreceptor and optogenetics research
University of Oregon
2012-2023
University of California, Davis
2001-2004
University of New Mexico
2001-2002
Center for Neurosciences
2002
University of Padua
1995-1999
The trafficking of glutamate receptors in neurons is the utmost importance for synapse formation and synaptic plasticity. Recently, we demonstrated that both NMDA AMPA reside mobile transport packets are recruited rapidly independently to nascent synapses. Here, show a large proportion receptor clusters young cortical present on surface dendrites before synapses formed these surface-exposed mobile. Exocytosis dendritic occurs via SNARE [soluble n-ethylmaleimide-sensitive factor attachment...
Vesicle-associated membrane protein (VAMP) (or synaptobrevin), a type II of small synaptic vesicles, is essential for neuroexocytosis because its proteolysis by tetanus and botulinum neurotoxins types B, D, F G blocks neurotransmitter release. The addition cross-linking reagents to isolated vesicles induces the formation 30 50 kDa complexes containing isoform 2 VAMP (VAMP-2). Whereas band VAMP-2 homodimer, species results from with synaptophysin. This heterodimer also forms in...
The cell adhesion molecule pair neuroligin1 (Nlg1) and beta-neurexin (beta-NRX) is a powerful inducer of postsynaptic differentiation glutamatergic synapses in vitro. Because Nlg1 induces accumulation two essential components the density (PSD) - PSD-95 NMDA receptors (NMDARs) can physically bind NMDARs at mature synapses, it has been proposed that recruits to through its interaction with PSD-95. However, are recruited nascent independently not known if accumulates before these PSD proteins....
To understand the molecular mechanisms of development it is essential to be able turn genes on and off at will in a spatially restricted fashion. Morpholino oligonucleotides (MOs) are very common tools used several model organisms with which possible block gene expression. Recently developed photo-activated MOs allow control over onset MO activity. However, deactivation photo-cleavable activity has remained elusive. Here, we describe activate or de-activate function by UV exposure temporal...
Host-associated microbiotas guide the trajectory of developmental programs, and altered microbiota composition is linked to neurodevelopmental conditions such as autism spectrum disorder. Recent work suggests that modulate behavioral phenotypes associated with these disorders. We discovered zebrafish required for normal social behavior reveal a molecular pathway linking microbiota, microglial remodeling neural circuits, in this experimentally tractable model vertebrate. Examining neuronal...
Botulinum neurotoxins type A and E (BoNT/A BoNT/E) are metalloproteases with a unique specificity for SNAP‐25 (synaptosome‐associated protein of 25 kDa), an essential component the neuroexocytotic machinery. It has been suggested that this is directed through recognition nine residue sequence, termed SNARE motif, common to other two proteins: VAMP (vesicle‐associated membrane protein) syntaxin, only known substrates six clostridial neurotoxins. Here we analyse involvement four copies motif...
The discovery that neuroligin is a key protein involved in synapse formation offers the unprecedented opportunity to induce functional synapses between neurons and heterologous cells. We took this recording for first-time synaptic currents human embryonic kidney 293 (HEK293) cells transfected with N-methyl-d-aspartate or AMPA receptor subunits co-culture rat cerebellar granule These were similar recorded neurons, their decay kinetics was determined by postsynaptic subunit combination....
Abstract Characterizing connectivity in the spinal cord of zebrafish embryos is not only prerequisite to understanding development locomotion, but also necessary for maximizing potential genetic studies circuit formation this model system. During their first day development, show two simple motor behaviors. First, they coil trunks spontaneously, and a few hours later start responding touch with contralateral coils. These behaviors are contemporaneous until spontaneous coils become infrequent...
Usher syndrome is the most prevalent cause of hereditary deaf-blindness, characterized by congenital sensorineural hearing impairment and progressive photoreceptor degeneration beginning in childhood or adolescence. Diagnosis management this disease are complex, molecular changes underlying sensory cell remain poorly understood. Here we characterize two zebrafish models for a severe form syndrome, type 1C (USH1C): one model mutant with newly identified ush1c nonsense mutation, other...
Abstract Usher syndrome (USH), the leading cause of hereditary combined hearing and vision loss, is characterized by sensorineural deafness progressive retinal degeneration. Mutations in several different genes produce USH, but proximal sensory cell death remains mysterious. We adapted a proximity ligation assay to analyze associations among three USH proteins, Cdh23, Harmonin, Myo7aa, microtubule based transporter Ift88 zebrafish inner ear mechanosensory hair cells. found that proteins are...
Zebrafish (Danio rerio) are highly social animals that engage in a diverse variety of nonreproductive behaviors emerge as early 14 days postfertilization (dpf). However, we observe considerable behavioral variability at this stage, and comparisons across studies potentially complicated both by chronological gaps measurements inconsistencies developmental staging. To address these issues, adapted our assay for orienting cueing the adult zebrafish used it to probe behavior critical window...
The release of neurotransmitter at a synapse occurs via the regulated fusion synaptic vesicles with plasma membrane. two lipid bilayers is mediated by protein complex that includes membrane target soluble N-ethylmaleimide-sensitive (NSF) attachment (SNAP) receptors (t-SNAREs), syntaxin 1A and synaptosome-associated 25kDa (SNAP-25), vesicle SNARE (v-SNARE), vesicle-associated (VAMP). Whereas VAMP are tethered to C-terminal transmembrane domain, SNAP-25 has been suggested be anchored four...
Synapse formation is a complex process that involves the recruitment and assembly of myriad pre- postsynaptic proteins. Despite being present at every synapse in vertebrate CNS, little known about transport, recruitment, stabilization synapsin nascent synapses during development. We examined transport to presynaptic terminals vivo developing zebrafish spinal cord. Synapsin was transported packet independently two other organelles: synaptic vesicle (SV) protein vesicles (STVs)...
The release of neurotransmitter at a synapse occurs via the regulated fusion synaptic vesicles with plasma membrane. two lipid bilayers is mediated by protein complex that includes membrane target soluble N-ethylmaleimide-sensitive (NSF) attachment (SNAP) receptors (t-SNAREs), syntaxin 1A and synaptosome-associated 25kDa (SNAP-25), vesicle SNARE (v-SNARE), vesicle-associated (VAMP). Whereas VAMP are tethered to C-terminal transmembrane domain, SNAP-25 has been suggested be anchored four...
Finding the link between behaviors and their regulatory molecular pathways is a major obstacle in treating neuropsychiatric disorders. The immediate early gene (IEG) EGR1 implicated etiology of disorders, linked to associated with social behavior. Despite extensive knowledge regulation at level, it remains unclear how deficits might affect component these Here, we examined behavior zebrafish mutation homologous egr1 Mutant fish exhibited reduced approach orienting, whereas other sensorimotor...
Artificial microRNA (amiRNA) sequences embedded in natural (miRNA) backbones have proven to be useful tools for RNA interference (RNAi). amiRNAs reduced off-target and toxic effects compared other RNAi-based methods such as short-hairpin RNAs (shRNA). are often less effective knockdown, however, their shRNA counterparts. We screened a large empirically-designed amiRNA set the synthetic inhibitory BIC/miR-155 (SIBR) scaffold show common structural sequence-specific features associated with...
In vitro studies suggest that the intracellular C terminus of Neuroligin1 (NL1) could play a central role in maturation excitatory synapses. However, it is unknown how this activity affects synapses vivo , and whether may impact development complex behaviors. To determine NL1 influences state glutamatergic we compared synaptic behavioral phenotypes mice overexpressing full-length version (NL1FL) with missing part domain (NL1ΔC). We show overexpression yielded an increase proportion mature...