A5091540497- Aortic Disease and Treatment Approaches
- Connective tissue disorders research
- Aortic aneurysm repair treatments
- Genetics, Aging, and Longevity in Model Organisms
- Virus-based gene therapy research
- FOXO transcription factor regulation
- Cardiovascular Issues in Pregnancy
- Nanoparticle-Based Drug Delivery
- Sirtuins and Resveratrol in Medicine
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Graphene and Nanomaterials Applications
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Pancreatitis Pathology and Treatment
- Adenosine and Purinergic Signaling
- Advanced Proteomics Techniques and Applications
- Neurobiology and Insect Physiology Research
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Cancer Genomics and Diagnostics
- Advanced Drug Delivery Systems
- Polysaccharides and Plant Cell Walls
- RNA Interference and Gene Delivery
Oregon Health & Science University
2010-2025
Shriners Hospitals for Children - Portland
2017-2023
University of Oregon
2009
In humans, mutations in fibrillin-1 result a variety of genetic disorders with distinct clinical phenotypes. While most the known cause Marfan syndrome, number other lead to features unrelated syndrome. Pathogenesis syndrome is currently thought be driven by mechanisms due haploinsufficiency wild-type fibrillin-1. However, haploinsufficiency-driven cannot explain phenotypes found fibrillinopathies. To test hypothesis that through primary effects on microfibril structure, two different were...
Abstract Characterizing connectivity in the spinal cord of zebrafish embryos is not only prerequisite to understanding development locomotion, but also necessary for maximizing potential genetic studies circuit formation this model system. During their first day development, show two simple motor behaviors. First, they coil trunks spontaneously, and a few hours later start responding touch with contralateral coils. These behaviors are contemporaneous until spontaneous coils become infrequent...
Background Sex-related differences as well the adverse effect of pregnancy on aortic disease outcome are well-established phenomena in humans with Marfan syndrome (MFS). The underlying mechanisms these observations largely unknown. Objectives In an initial (pilot) step we aimed to confirm between male and female MFS patients females without previous pregnancy. We then sought evaluate whether findings recapitulated a pre-clinical model performed in-depth cardiovascular phenotyping mutant both...
Senescent cells accumulate in tissues over time as part of the natural ageing process and removal senescent has shown promise for alleviating many different age-related diseases mice. Cancer is an age-associated disease there are numerous mechanisms driving cellular senescence cancer that can be detrimental to recovery. Thus, it would beneficial develop a senolytic acts not only on but also prevent recurrence or progression.We used molecular modelling series rationally designed peptides...
BackgroundPancreatic ductal adenocarcinoma (PDAC) is a highly intratumorally heterogeneous disease that includes several subtypes and plastic. Effective gene delivery to all PDAC cells essential for modulating expression identifying potential gene-based therapeutic targets in PDAC. Most current systems pancreatic are optimized islet or acinar cells. Lentiviral vectors the main PDAC, but their transduction efficiencies vary depending on cell type, especially poor classical subtype of from...
Background & Aims: Pancreatitis is an inflammatory disease of the exocrine pancreas and a known risk factor for pancreatic ductal adenocarcinoma (PDAC). We previously identified HMG-box transcription 1 (HBP1) as potential master (TF) involved in early progression PDAC associated with poor patient survival. However, functional role HBP1 pathophysiological mechanism from acute to chronic pancreatitis remains unclear. Methods: examined expression human samples cerulein-induced mouse model....
Pancreatic ductal adenocarcinoma (PDAC) exhibits extensive inter- and intratumoral heterogeneity, along with a significant stromal component. This protocol outlines steps to generate patient-derived xenografts (PDXs), isolate enrich human PDAC epithelial cells, verify their identity using droplet digital PCR (ddPCR) human-specific markers. It provides robust approach for culturing cancer cells develop model system. For complete details on the use execution of this protocol, please refer...
Immunolocalization studies have shown that fibrillin-1 is distributed ubiquitously in the connective tissue space from early embryonic times through old age. When mutated, gene for (FBN1) causes Marfan syndrome, a common inherited disorder of tissue. The multiple manifestations syndrome reflect known distribution cardiovascular, musculoskeletal, ocular, and dermal tissues. In this study, mouse model which truncated tagged with green fluorescence was used to estimate relative abundance...
Some cancer cells rely heavily on non-essential biomolecules for survival, growth, and proliferation. Enzyme based therapeutics can eliminate these biomolecules, thus specifically targeting neoplastic cells; however, enzyme are susceptible to immune clearance, exhibit short half-lives, require frequent administration. Encapsulation of therapeutic cargo within biocompatible biodegradable poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) is a strategy controlled release. Unfortunately,...
Objective Tobacco smoke exposure is a major risk factor for aortic aneurysm development. However, the initial response to tobacco smoke, preceding formation, not well understood. We sought create model determine effect of solubilized (STS) on thoracic and abdominal aorta mice as cultured human smooth muscle cells (HASMCs). Methods was delivered via implanted osmotic minipumps. Twenty male C57BL/6 received STS or vehicle infusion. The descending thoracic, suprarenal abdominal, infrarenal...
Proteases are promising biomarkers for cancer early detection. Their enzymatic activity against peptide substrates allows their straightforward detection using low-cost tests. However, the complexity of human proteome makes it challenging to develop sensitive and selective tests a specific protease biomarker. Here, we report different approach by utilizing total in plasma samples detect pancreatic cancer. Instead targeting substrate, utilized an array 360 FRET screen cleavage patterns...
Pancreatic ductal adenocarcinoma (PDAC) is highly intratumorally heterogeneous and has several subtypes. Effective gene delivery to all PDAC cells essential for modulating expression identifying potential gene-based therapeutic targets in PDAC. Most current systems pancreatic are optimized islet or acinar cells. Lentiviral vectors the main PDAC, but their transduction efficiencies vary depending on cell types, especially poor classical subtype of from both primary tumors lines. Herein, we...
Abstract Lungs are a frequent site for disease but difficult to image and probe, potentially exacerbating lung disease, delaying diagnoses impacting survival. Here, we demonstrate low cost, minimally invasive method probe the lungs using genetically engineered Lactiplantibacillus plantarum strain WCFS1. Genetically modified WCFS1 was delivered specifically of mice, where it shown remain transcriptionally active several hours then be cleared without further colonization. secrete...
Background: Senescent cells accumulate in tissues over time as part of the natural aging process and removal senescent has shown promise for alleviating many different age-related diseases mice. Cancer is an age-associated disease there are numerous mechanisms driving cellular senescence cancer that can be detrimental to recovery. Thus, it would beneficial eliminate prevent recurrence or progression. Methods: We used molecular modeling develop a series rationally designed peptides mimic...
Some cancer cells rely heavily on non-essential biomolecules for survival, growth, and proliferation. Enzyme based therapeutics can eliminate these biomolecules, thus specifically targeting neoplastic cells; however, enzyme are susceptible to immune clearance, exhibit short half-lives, require frequent administration. Encapsulation of therapeutic cargo within biocompatible biodegradable poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) is a strategy controlled release. Unfortunately,...