A5076666185- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Viral Infections and Outbreaks Research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Bacterial Infections and Vaccines
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Muscle Physiology and Disorders
- Planarian Biology and Electrostimulation
- Vaccine Coverage and Hesitancy
- Sepsis Diagnosis and Treatment
- Infection Control and Ventilation
- Immune responses and vaccinations
- Immunodeficiency and Autoimmune Disorders
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Insect and Pesticide Research
- Inhalation and Respiratory Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Viral Infections and Immunology Research
Ospedale Sacro Cuore Don Calabria
2022-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2025
University of Trento
2021-2023
A febrile man in Italy who had traveled to Cuba July 2024 was diagnosed with Oropouche fever. Reverse transcription PCR detected prolonged shedding of virus RNA whole blood, serum, urine, and semen. Sixteen days after symptom onset, replication-competent semen, suggesting risk for sexual transmission.
Currently, evaluation of the IgG antibodies specific for SARS-CoV-2 Spike protein following vaccination is used worldwide to estimate vaccine response. Limited data are available on vaccine-elicited IgM and their potential implication in immunity SARS-CoV-2.
On 27 May 2024, the Cuban Ministry of Health reported first outbreak Oropouche fever on island. The etiologic agent, virus (OROV), is a poorly understood arbovirus that has been known since 1960s and represents public health burden in Latin America. We report whole-genome characterization European OROV isolate from returning traveler Cuba with fever-like symptoms. was obtained patient's serum; sequencing performed by next-generation sequencing, followed phylogenetic analysis genetic...
Toscana virus (TOSV), a sandfly-borne virus, is an important etiological agent in human acute meningitis and meningoencephalitis the Mediterranean area during summer. However, actual number of TOSV infections underestimated. Laboratory confirmation necessary because infection has overlapping clinical features with other neuro-invasive viral infections. Nowadays, reference test for direct diagnosis phase PCR based method detecting cerebrospinal fluid and/or plasma, serum, or blood. Although...
Currently approved vaccines are highly effective in protecting against hospitalization and severe COVID-19 infections. How pre-existing immunity responds to new variants with mutated antigens is crucial information for elucidating the functional interplay between antibodies B T cell responses during infection SARS-CoV-2 variants.
Two women returning from Cuba had persistent Oropouche virus (OROV) viremia on whole-blood samples at ten and twelve weeks after symptoms onset. This finding underscores the role of specimens for OROV molecular detection, raises concern regarding risks in transplantation, blood donation timing safe conception infection.
Our study wanted to describe the kinetics of serological and adaptive immune responses in mpox patients. Methods: Fourteen patients with laboratory-confirmed were tested at different time points after symptom onset. An immunofluorescence assay was performed evaluate seroconversion specific IgA, IgM, IgG. Moreover, characterization immunological profile T- B-cells performed. Results: The antibody revealed faster more effective IgA than IgM. we detected an increase Active memory B cells CD8+...
ABSTRACT Splenectomy or congenital asplenia is associated with severe reduction of memory B cells and increased risk fulminant sepsis by encapsulated bacteria. Current guidelines recommend vaccinations against these pathogens before after splenectomy, but the longevity immunity acquired splenectomy has not been determined. The impact on innate immune unknown. We analyzed frequency, differentiation stage, function adaptive in peripheral blood adult ( n = 41) pediatric 14) patients...
The antibody response to SARS-CoV-2 mRNA vaccines in individuals with waning immunity generated by a previous infection, as well the patterns of IgA and IgM responses previously infected naïve are still poorly understood.
Introduction Assessing the response to vaccinations is one of diagnostic criteria for Common Variable Immune Deficiencies (CVIDs). Vaccination against SARS-CoV-2 offered unique opportunity analyze immune a novel antigen. We identify four CVIDs phenotype clusters by integration parameters after BTN162b2 boosters. Methods performed longitudinal study on 47 patients who received 3rd and 4th vaccine dose BNT162b2 measuring generation immunological memory. analyzed specific neutralizing...
The vaccination campaign against SARS-CoV-2 relies on the world-wide availability of effective vaccines, with a potential need 20 billion vaccine doses to fully vaccinate world population. To reach this goal, manufacturing and logistic processes should be affordable all countries, irrespective economical climatic conditions. Outer membrane vesicles (OMVs) are bacterial-derived that can engineered incorporate heterologous antigens. Given inherent adjuvanticity, such modified OMVs used as...
In 2022, an unprecedented outbreak of mpox raged in several nations. Sequences from the 2022 reveal a higher nucleotide substitution if compared with estimated rate for orthopoxviruses. Recently, intra-lesion SNVs (single variants) have been described, and these suggested as possible sources genetic variation. Until now, it has not clear presence could represents result local mutagenesis or co-infection. We investigated significance through whole-genome sequencing analysis four unrelated...
Monkeypox virus (MPXV) is a double-stranded DNA belonging to the orthopoxvirus genus in family Poxviridae. Distinct clades are identified: clade I Central African (or Congo Basin) and subclades IIa IIb West clade. Here, commercial droplet digital PCR (ddPCR) assay was evaluated for quantification of MPXV Africa clinical samples.The ddPCR reaction assessed as duplex using RPP30 an internal amplification control. A total 60 specimens were tested, 40 positives (skin lesions, n=10; rectal swabs,...
The vaccination campaign against SARS-CoV-2 relies on the world-wide availability of effective vaccines, with a potential need 20 billion vaccine doses to fully vaccinate world population. To reach this goal, manufacturing and logistic processes should be affordable all countries, irrespectively economical climatic conditions. Outer membrane vesicles (OMV) are bacterial-derived that can engineered incorporate heterologous antigens. Given inherent adjuvanticity, such modified OMV used as...
Background: Oropouche virus (OROV) is a segmented RNA belonging to the genus Orthobunyavirus in family Peribunyaviridae. Herein, an in-house droplet digital PCR (ddPCR) assay was used for detection and quantification of OROV. Methods: The ddPCR reaction assessed as duplex using human housekeeping gene RPP30. Limit (LoD) analysis performed whole blood, serum, urine. executed on total 28 clinical samples (whole blood n = 9, serum 11, urine 8), which 16 specimens were tested positive at routine...
Skeletal muscle is composed of syncytial fibers, and by various mononucleated cellular types, such as stem cells, immune interstitial stromal progenitors. These cell populations play a crucial role during regeneration, alterations their phenotypic properties have been associated with defective repair fibrosis in aging dystrophic muscle. Studies involving vivo gene modulation are valuable to investigate the mechanisms underlining function dysfunction complex pathophysiological settings....
Abstract We profiled antibody responses in a cohort of recipients the BTN162b2 mRNA vaccine who were either immunologically naïve (n=50) or had been previously infected with SARS-CoV-2 (n=51). Of infected, 25 and 26 during first second pandemic waves Italy, respectively; majority those from wave corresponding waning immunity low to undetectable levels anti-S antibodies anti-N antibodies. observed that spike-specific IgG pseudovirus neutralization titers rapidly recalled by single dose higher...
Background: IgG antibodies specific for the SARS-CoV-2 Spike protein are under intensive investigation urgent need to identify a correlate of protective immunity in individuals vaccinated with licensed and candidate COVID-19 vaccines. Limited data available on vaccine-elicited IgM their potential implication SARS-CoV-2. Methods: We performed longitudinal study quantify spike (IgG-S IgM-S) 1873 health care worker (HCW) recipients BNT162b2 (Comirnaty) vaccine. Samples were collected before...