A5061325274- Glioma Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Neuroblastoma Research and Treatments
- Chromatin Remodeling and Cancer
- Sarcoma Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Bladder and Urothelial Cancer Treatments
- Neuroendocrine Tumor Research Advances
- Ocular Oncology and Treatments
- Cancer-related molecular mechanisms research
- Neurofibromatosis and Schwannoma Cases
- Colorectal Cancer Screening and Detection
- Endoplasmic Reticulum Stress and Disease
- Medical Imaging and Pathology Studies
- Bone Tumor Diagnosis and Treatments
- Renal and related cancers
- Liver physiology and pathology
- Protein Degradation and Inhibitors
- Viral-associated cancers and disorders
- Pancreatic and Hepatic Oncology Research
- Protein Structure and Dynamics
University of California, San Francisco
2015-2025
UCSF Helen Diller Family Comprehensive Cancer Center
2006-2024
Genomics (United Kingdom)
2020
Emory University Hospital
2020
Lucas Research
2020
San Francisco General Hospital
2009-2015
University of California San Francisco Medical Center
2015
Smith-Kettlewell Eye Research Institute
2009
Massachusetts General Hospital
2008
Royal Victoria Hospital
2008
Many functions of the chaperone, heat shock protein 90 (hsp90), are inhibited by drug geldanamycin that specifically binds hsp90. We have studied an amino-terminal domain hsp90 whose crystal structure has recently been solved and determined to contain a geldanamycin-binding site. demonstrate that, in solution, binding is exclusive this domain. This also ATP linked Sepharose through γ-phosphate. Binding specific for ADP geldanamycin. Mutation four glycine residues within two proposed motifs...
Molecular profiling is revolutionizing cancer diagnostics and leading to personalized therapeutic approaches. Herein we describe our clinical experience performing targeted sequencing for 31 pediatric neuro-oncology patients.We sequenced 510 cancer-associated genes from tumor peripheral blood identify germline somatic mutations, structural variants, copy number changes.Genomic was performed on patients with tumors including 11 high-grade gliomas, 8 medulloblastomas, 6 low-grade 1 embryonal...
Matrix rigidity has important effects on cell behavior and is increased during liver fibrosis; however, its effect primary hepatocyte function unknown. We hypothesized that matrix in fibrotic livers would activate mechanotransduction hepatocytes lead to inhibition of liver-specific functions. To determine the physiologically relevant ranges stiffness at cellular level, we performed detailed atomic force microscopy analysis across lobules from normal livers. determined was around 150 Pa 1-6...
The chaperone hsp90 is capable of binding and hydrolyzing ATP. Using information on a related ATPase, DNA gyrase B, we selected three conserved residues in hsp90's ATP-binding domain for mutation. Two these mutations eliminate nucleotide binding, while the third retains but apparently deficient ATP hydrolysis. We first analyzed how affect to co-chaperones p23 Hop, hydrophobic resin, phenyl-Sepharose. These experiments showed that ATP's effects, specifically, increased affinity decreased Hop...
Ganglioglioma is the most common epilepsy-associated neoplasm that accounts for approximately 2% of all primary brain tumors. While a subset gangliogliomas are known to harbor activating p.V600E mutation in BRAF oncogene, genetic alterations responsible remainder largely unknown, as spectrum any additional cooperating gene mutations or copy number alterations. We performed targeted next-generation sequencing provides comprehensive assessment mutations, fusions, and on cohort 40...
Abstract Pleomorphic xanthoastrocytoma ( PXA ) is an astrocytic neoplasm that typically well circumscribed and can have a relatively favorable prognosis. Tumor progression to anaplastic WHO grade III ), however, associated with more aggressive biologic behavior worse The factors drive are largely unknown. We performed comprehensive genomic profiling on set of 23 s from 19 patients, including 15 . Four patients had tumor tissue multiple recurrences, two progression. find genetically defined...
<h3>Importance</h3> Papillary thyroid carcinoma (PTC) is the most common endocrine neoplasm. B-type raf kinase (<i>BRAF</i>) V600E mutation has been proposed as a negative prognostic indicator in PTC, and patients harboring it should receive more aggressive initial therapy. <h3>Objective</h3> To assess significance of<i>BRAF</i>V600E PTC largest US sample to date. <h3>Design</h3> We identified from our institution's pathology archives diagnosed having meeting criteria for<i>BRAF</i>mutation...
Abstract High‐grade neuroepithelial tumor with BCOR exon 15 internal tandem duplication (HGNET ex15 ITD) is a recently proposed entity of the central nervous system (CNS) distinct methylation profile and characteristic genetic alteration. The complete spectrum histologic features, accompanying alterations, clinical outcomes, optimal treatment for this new are largely unknown. Here, we performed comprehensive assessment 10 cases HGNET ITD. tumors mostly occurred in young children were located...
Metastatic tumors with an uncertain primary site can be a difficult clinical problem. In tens of thousands patients every year, no confident diagnosis is ever issued, making standard-of-care treatment impossible. Gene expression profiling (GEP) tests currently available to analyze these difficult-to-diagnose have never been directly compared the diagnostic standard care, immunochemistry (IHC). This prospectively conducted, blinded, multicenter study compares accuracy GEP IHC in identifying...
Mesonephric remnants of the cervix are vestiges embryonic mesonephric system which typically regresses during female development. Uncommonly, hyperplasia may occur. The differential diagnosis exuberant includes minimal deviation adenocarcinoma cervix, a tumor with deceptively bland morphology for no reliable diagnostic biomarkers currently exist. PAX2 encodes transcription factor necessary in development Wolffian duct system, and protein is expressed several tumors origin, including renal...
A subset of women with uterine cancer exhibiting defective mismatch repair (MMR) proteins and microsatellite instability (MSI) may have Lynch syndrome, which also confers a risk for colorectal other cancers in the patient her family. Screening algorithms based on clinical pathologic criteria are effective determining patients most likely to benefit from definitive genetic testing syndrome. Ovarian cancer, particularly endometrioid adenocarcinoma, is associated although much smaller than...
Abstract Chordoid glioma is a rare brain tumor thought to arise from specialized glial cells of the lamina terminalis along anterior wall third ventricle. Despite being histologically low-grade, chordoid gliomas are often associated with poor outcome, as their stereotypic location in ventricle makes resection challenging and efficacious adjuvant therapies have not been developed. Here we performed genomic profiling on 13 identified recurrent D463H missense mutation PRKCA all tumors, which...
Abstract “Myxoid glioneuronal tumor, PDGFRA p.K385‐mutant” is a recently described tumor entity of the central nervous system with predilection for origin in septum pellucidum and defining dinucleotide mutation at codon 385 oncogene replacing lysine either leucine or isoleucine (p.K385L/I). Clinical outcomes optimal treatment this new have yet to be defined. Here, we report comprehensive clinical, radiologic, histopathologic assessment eight cases. In addition its stereotypic location...
Methionine-choline-deficient (MCD) diets cause steatohepatitis in rodents and are used to study the pathophysiology of fatty liver disease human beings. The most widely commercial MCD formulas not only lack methionine choline but also contain excess sucrose fat. objective this was determine whether dietary formula plays a role pathogenesis MCD-related disease. We prepared two custom formulas, one containing as principal carbohydrate other substituting with starch. Mice fed sucrose-enriched...