A5037816950- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- Physiological and biochemical adaptations
- Insect Utilization and Effects
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- Insect-Plant Interactions and Control
- Silk-based biomaterials and applications
- Heat shock proteins research
- Animal Behavior and Reproduction
- Vector-Borne Animal Diseases
- RNA modifications and cancer
- Insect Pest Control Strategies
- Genetics, Aging, and Longevity in Model Organisms
- Animal Genetics and Reproduction
- Hemiptera Insect Studies
- Forensic Entomology and Diptera Studies
- Study of Mite Species
- Plant Molecular Biology Research
- Orthoptera Research and Taxonomy
- Silkworms and Sericulture Research
- Protein Tyrosine Phosphatases
Institute of Agrobiological Sciences
2014-2025
National Agriculture and Food Research Organization
2017-2025
The University of Tokyo
2005-2010
The Krüppel homolog 1 gene ( Kr-h1 ) has been proposed to play a key role in the repression of insect metamorphosis. is assumed be induced by juvenile hormone (JH) via JH receptor, methoprene-tolerant (Met), but mechanism induction unclear. To elucidate molecular induction, we first cloned cDNAs encoding BmKr-h1 and Met BmMet1 BmMet2 homologs from Bombyx mori . In B. cell line, was rapidly subnanomolar levels natural JHs. Reporter assays identified response element k JHRE), comprising 141...
In insects, the precise timing of molting and metamorphosis is strictly guided by a principal steroid hormone, ecdysone. Among multiple conversion steps for synthesizing ecdysone from dietary cholesterol, 7-dehydrocholesterol to 5beta-ketodiol, so-called 'Black Box', thought be important rate-limiting step. Although number genes essential synthesis have recently been revealed, much less known about that are crucial functioning in Black Box. Here we report on novel ecdysteroidgenic gene,...
Juvenile hormone (JH) represses precocious metamorphosis of larval to pupal and adult transitions in holometabolous insects. The early JH-inducible gene Krüppel homolog 1 (Kr-h1) plays a key role the repression as mediator JH action. Previous studies demonstrated that Kr-h1 inhibits larval-pupal transition immature larva via direct transcriptional specifier Broad-Complex (BR-C). was recently reported repress Ecdysone-induced protein 93F (E93); however, its mechanism action remains unclear....
The red flour beetle, Tribolium castaneum, has been widely used as a laboratory model for analyzing gene function. In this study, we established novel cell line (Tc81) from T. castaneum embryos and validated the utility of by juvenile hormone (JH) signaling pathway. Tc81 cells, Krüppel homolog 1 (Kr-h1), which is JH-dependent repressor insect metamorphosis, was rapidly induced subnanomolar levels JHs. Bioinformatics analysis reporter assays identified 2 JH response elements (kJHREs) located...
Recent studies in vitro have reported that the Methoprene-tolerant (Met) and Taiman (Tai) complex is functional receptor of juvenile hormone (JH). Experiments vivo Met depletion confirmed this factor's role JH signal transduction, however, there no equivalent data regarding Tai because its larval or nymphal stages beetle Tribolium castaneum bug Pyrrhocoris apterus results 100% mortality. We discovered cockroach Blattella germanica possesses four isoforms resulting from combination two indels...
Abstract The full‐length Hsp90 cDNA in Delia antiqua was cloned and sequenced. deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer‐ winter‐diapauses both elevated HSP90 transcript levels D. pupae. Levels gradually increased time summer diapausing pupae whereas fluctuated winter Cold‐ heat‐stressing summer‐ winter‐diapausing individuals further expression. mRNA decreased after an initial increase expression also up‐regulated following cold‐...
The cytosolic members of the HSP70 family proteins play key roles in molecular chaperone machinery cell. In study we cloned and sequenced fulllength cDNA Delia antiqua gene, which is 2461 bp long encodes 643 a.a. with a calculated mass 70,787 Da. We investigated gene copies 4 insect species complete genome available, found that they are quite variable species. order to characterize this protein carried out an alignment phylogenetic analysis 41 sequences from insects. divided into two...
Winter-diapause and cold-acclimated non-diapause pupae of the onion maggot, Delia antiqua (Diptera: Anthomyiidae), show strong cold hardiness. To obtain insights into mechanisms involved in enhancement hardiness, we investigated expression patterns genes encoding subunits chaperonin (CCT) morphology actin, a substrate CCT, at low temperatures. Quantitative real-time PCR analyses showed mRNA levels CCT pupal tissues to be highly correlated with hardiness pupae. While actin Malpighian tubules...
Abstract Transgenic techniques offer a valuable tool for determining gene functions. Although various promoters are available use in overexpression, knockdown, and identification of transgenic individuals, there is nevertheless lack versatile such studies, this dearth acts as bottleneck, especially with regard to nonmodel organisms. Here, we succeeded identifying novel strong ubiquitous promoter/enhancer the silkworm. We identified unique silkworm strain whose reporter showed expression...
Insecticide resistance has recently become a serious problem in the agricultural field. Development of insecticides with new mechanisms action is essential to overcome this limitation. Juvenile hormone (JH) an insect-specific that plays key roles maintaining larval stage insects. Hence, JH signaling pathway considered suitable target development novel insecticides; however, only few inhibitors (JHSIs) have been reported, and no practical JHSIs developed. Here, we established high-throughput...
Patanga japonica (Bolívar) shows various body colors in the field. Most nymphs are green summer, but some develop non-green colors, such as yellow, white, brown, reddish, and black, fall. Nymphs individually reared yellow-green, black containers showed green, light-green, reddish substrate color significantly influenced proportions of nymphs. A few individuals developed spots patterns, were most frequently observed containers. with distinct patterns when a group five per container,...