A5023701978- Periodontal Regeneration and Treatments
- Tendon Structure and Treatment
- Fuel Cells and Related Materials
- Membrane Separation and Gas Transport
- Laser Applications in Dentistry and Medicine
- Mechanical Circulatory Support Devices
- Knee injuries and reconstruction techniques
- Corneal Surgery and Treatments
- Blood properties and coagulation
- COVID-19 Clinical Research Studies
- Corneal surgery and disorders
- Inflammasome and immune disorders
- Vitamin C and Antioxidants Research
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Orthopedic Surgery and Rehabilitation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Wound Healing and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer Risks and Factors
- Tissue Engineering and Regenerative Medicine
- Hemophilia Treatment and Research
- Regional Economic Development and Innovation
- Laser Material Processing Techniques
- Blood transfusion and management
Research Institute of Emergency Care
2013-2025
Background. In recent decades, the frequency of intrauterine pathology (IUP) has increased: endometrial hyperplasia and polyps, submucous uterine fibroids (SMUF), other conditions that affect 38–72% women reproductive age. An imbalance in cell proliferation, differentiation, death plays an important role pathogenesis IUP. Aim. To study assess proteins regulating apoptosis proliferation patients age with various IUPs. Materials methods. We analyzed comprehensive examination data 87 (mean...
Introduction. The thromboelastography method potentially can be used to assess the hemostatic potential of platelet concentrates (PC). Currently, in vitro thromboelastographic study PC is not standardized. aim work. To evaluate quality by a medium, similar whole blood. Materials and methods. We examined blood samples from donors, PC, harvested apheresis, obtained pooling fractions, isolated donor compare data clinical efficacy, PC-doses were transfused 11 patients for relief or prevention...
During the last two years, treatment of patients with novel coronavirus infection COVID-19 remains an urgent health problem. Interferon proteins are known to play a significant role in antiviral immunity. Some pathological conditions accompanied by production neutralizing autologous immunoglobulins against own host interferons (auto-IFN-Abs). There is evidence that autoantibodies alpha and omega detected life-threatening course pneumonia. The aim our study was analyze prevalence interferon...
The aim of the investigation was to study possibilities evaluating biological properties various tissue grafts analyzing autofluorescence level their collagen fibers.Materials and Methods.The involved types standard-produced grafts, some them being exposed additional treatment stimulate changes in fiber structure.Analysis preparations made using light, fluorescent confocal microscopy.To evaluate human cell adhesion on with different levels, M-22 fibroblasts blood platelets were...
<h3></h3> Тяжесть течения новой коронавирусной инфекции (COVID-19), вызванной вирусом SARS-CoV-2, во многом обусловлена развитием у больных иммунопатологического состояния. У 85% пациентов с тяжелым течением COVID-19 отмечается лимфопения, развитие которой может быть связано повышенным апоптозом лимфоцитов. <h3>ЦЕЛЬ ИССЛЕДОВАНИЯ</h3> Провести анализ апоптотической гибели лимфоцитов и изменений белков — регуляторов программируемой смерти клеток COVID-19. <h3>МАТЕРИАЛ И МЕТОДЫ</h3> Обследованы...
The research objective was to study the reparative effects of collagen type I bio-construct loaded with buccal epithelial cells, on rabbit cornea after experimental keratectomy at various stages treatment (on 3rd, 7th, 14th, 3 0th days). Material and methods . experiments were conducted 20 rabbits Chinchilla breed that operated both eyes aiming inflict stromal defects. collagen-based bearing cells placed over eyes. control covered smooth contact lens. After surgery, a temporal...
Purpose: To study the biological effect of a combination platelet lysate and amniotic membrane, preserved by various techniques, on human buccal epithelium culture. Materials methods. Human amnion transplants were using 3 methods: silicate drying, lyophilization, cryopreservation. The blood healthy volunteers was used as source platelets. Platelet-rich plasma (PRP) with content over 1000 thousand/mcl more isolated from donors blood, frozen at -80 ° С defrosted 0–4 to prepare lysate....
Background. The improvement of treatment tactics for nosocomial pneumonia in patients with burn and inhalation trauma is a relevant issue due to the frequency this complication, which develops at any stage aggravates course underlying disease. Aim study. To evaluate effectiveness extremely high therapy (EHFT) complex injury. Material methods. results high-frequency was compared, studying 23 EHFT 30 without EHFT. Results . reduces signs endogenous toxemia, as evidenced by statistically...
Structural and functional properties of the human tendon tissue complicate process tendon-based grafts preserving. In preservation, it is necessary to maintain mechanical parameters as well provide sterility biological safety graft. One could conclude effectiveness techniques, combining conservation at low temperatures sterilization with supercritical carbon dioxide. Aim work : find optimal conditions for allogeneic Materials methods . Allogeneic tendons were prepared from donors, following...
Introduction . Biological grafts, including bone chips and collagen, are supposed to be promising in the treatment of tissue treatment. Nevertheless, manufacturing these grafts still needs standardized. Aim To optimize methodology osteoplastic material production, based on allogenic 1 type collagen chips. Material methods Osteoplastic were produced, using with allogeneic 180-800 µm solution acidic acid. We studied total integrity graft, quality, morphofunctional properties line M-22 human...
Purpose : to compare the effectiveness of conventional preserved amnion and saturated with platelet-rich (PRP) plasma lysate for treatment persistent corneal erosions (PCE) after high-risk keratoplasty. Materials methods . 40 patients penetrating keratoplasty, followed up 12 months, were divided into two clinical groups 20 people each. The main group patients, aged 34 84, received Flexamer amniotic membrane + PRP lysate, while comparison group, 41 80, only. Amniotic coating was used in...
Allogeneic tendon grafts are seriously demand in knee joint plastic surgery. The novel method of cryopreservation, including sterilization with supercritical carbon dioxide, was developed N.V. Sklifosovsky Research Institute for Emergency Cryopreserved tendons retain their normal fiber structure without significant loss mechanical properties. At the next stage it necessary to evaluate cryopreserved tendons′ integration inside bone canal experimental animals. aim study. To morphologic changes...
Introduction. Harvesting of pooled platelet concentrates (PC) significantly expands the volume transfusion support and could be used to create strategic reserve. The aim work . To compare quality apheresis concentrates. Materials methods. We studied fractions (PF), isolated from whole blood cadre donors with Reveos system, concentrates, obtained by pooling PF (Pooled PC), harvested automatic apheresis. In all cases 2 days passed moment donation examination. PC samples we cellular...
Experiments on 39 male Wistar rats were performed. A metal-organic coordination polymer modified with iron oxide and ascorbic acid (composite) was administered intravenously to healthy at doses of 25 50 mg/kg. The anti/prooxidant activity the composite assessed 3 24 hours after administration. in both shown have no significant effect homeostasis prooxidants/antioxidants blood serum animals. In high doses, enhances apoptosis lymphocytes, which indicates its possible use as a catalyst for free...
Purpose: to evaluate the content of tissue inhibitor matrix metalloproteinase type 1 (TIMP-1) in platelet preparations obtained different methods. Material and methods . Platelet-rich plasma (PRP), platelet-poor plasma, a suspension platelets washed from (WP) were isolated blood 10 volunteer donors, morphofunctional analysis was performed. After that, lysate prepared PRP, WP, plasma. The concentration TIMP-1 lysates determined using enzyme immunoassay. Results. level PRP WP did not differ...
Background : Controlled generation of active forms oxygen and nitrogen stimulates wound healing processes. One the sources controlled is low-temperature argon plasma. Low-temperature plasma activates various cellular activity. However, subtle mechanisms such stimulation remain unclear. Thus, there a need for detailed study biological effect on human cells. Objective to evaluate structure proliferative activity fibroblasts in vitro. Materials methods . Human fibroblast culture (line M-21,...