A5019224823- Cellular Mechanics and Interactions
- Mechanisms of cancer metastasis
- Microtubule and mitosis dynamics
- Axon Guidance and Neuronal Signaling
- Hippo pathway signaling and YAP/TAZ
- Lymphoma Diagnosis and Treatment
- Ion channel regulation and function
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Histone Deacetylase Inhibitors Research
- Viral-associated cancers and disorders
- Cancer Research and Treatments
- Hedgehog Signaling Pathway Studies
- Cell Adhesion Molecules Research
- Ferrocene Chemistry and Applications
- Cancer-related Molecular Pathways
- Cancer Mechanisms and Therapy
- Retinal Development and Disorders
- Angiogenesis and VEGF in Cancer
- Ion Transport and Channel Regulation
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Genetic and Kidney Cyst Diseases
University of Denver
2016-2025
Yale University
2025
University of Chinese Academy of Sciences
2019
Institute of Modern Physics
2019
Chinese Academy of Sciences
2019
First People's Hospital of Chongqing
2018
University of California San Francisco Medical Center
2017
Lanzhou University
2017
Lanzhou University Second Hospital
2017
Duke University Hospital
2016
Primary cilia (PC) are solitary, sensory organelles that critical for several signaling pathways. PC were detected by immunofluorescence of cultured cells and breast tissues. After growth 7 days in vitro, ∼70% fibroblasts 7–19% epithelial derived from benign (184A1 MCF10A). In 11 cancer cell lines, present at a low frequency four (from 0.3% to 4% cells), but absent the remainder. The lines with all basal B subtype, which is analogous clinical triple-negative subtype. Furthermore, decreased...
Oriented cell intercalation is an essential developmental process that shapes tissue morphologies through the directional insertion of cells between their neighbors. Previous research has focused on properties cell–cell interfaces, while function tricellular vertices remained unaddressed. Here, we identify a highly novel mechanism in which demonstrate independent sliding behaviors along peripheries to produce topological deformations responsible for intercalation. Through systematic...
The coordination between membrane trafficking and actomyosin networks is essential to the regulation of cell tissue shape. Here, we examine Rab protein distributions during Drosophila epithelial remodeling show that Rab35 dynamically planar polarized. compartments are enriched at contractile interfaces intercalating cells provide first evidence interfacial monopolarity. When function disrupted, apical area oscillations still occur steps observed. However, contractions followed by reversals...
Osteoclast activation is important for bone remodeling and altered in multiple disorders. This process requires cell adhesion extensive actin cytoskeletal reorganization. Proline-rich tyrosine kinase 2 (PYK2), a major adhesion–activated osteoclasts, plays an role regulating this event. The mechanisms by which PYK2 regulates organization osteoclastic remain largely unknown. In paper, we provide evidence that directly interacts with gelsolin, binding, severing, capping protein essential...
Recent genetic analysis has identified a pivotal role of primary cilia in the pathogenesis polycystic kidney disease (PKD). However, little is known regarding how loss/dysfunction contributes to cyst development. In epithelial cells, changes apical fluid flow induce cilia-mediated Ca2+ entry via polycystin-2 (PC2), cation channel. The Oak Ridge Polycystic Kidney (orpk) mouse contains mutated Tg737 gene that disrupts expression polaris, protein required for ciliogenesis. These studies examine...
One of the essential functions dendritic cells is to take up Ags in peripheral tissues and migrate into secondary lymphoid organs present lymphocytes for induction immune responses. Although many studies have demonstrated that migration closely associated with development responses, little known about factors inhibit cell control extent responses Ag stimulation. We show Slit2, a neuronal repellent factor, up-regulated skin by allergen sensitization down-regulates Langerhans cells. The effect...
Abstract Several molecules that suppress metastasis without suppressing tumorigenicity have been identified, but their mechanisms of action not yet determined. Many block growth at the secondary site, suggesting involvement in how cells respond to signals from extracellular milieu. Breast cancer suppressor 1 (BRMS1)–transfected MDA-MB-435 were examined for modifications phosphoinositide signaling as a potential mechanism suppression. 435/BRMS1 expressed <10% phosphatidylinositol-4,...
The BRMS1 metastasis suppressor interacts with the protein AT-rich interactive domain 4A (ARID4A, RBBP1) as part of SIN3·histone deacetylase chromatin remodeling complexes. These transcriptional co-repressors regulate diverse cell phenotypes depending upon complex composition. To define complexes and their roles in suppression, we generated mutants (BRMS1<sup>mut</sup>) mapped ARID4A interactions. BRMS1<sup>L174D</sup> disrupted direct interaction yeast two-hybrid genetic screens but...
Abstract Objectives: To examine interactions among clinical factors and pathologic biomarkers in predicting the outcome of patients with diffuse large B-cell lymphoma (DLBCL) treated rituximab-based immunochemotherapy. Methods: In 85 at a single institution, clinicopathologic variables were analyzed, including International Prognostic Index (IPI); germinal/nongerminal center phenotype; MYC, p53, BCL2, Ki-67, Epstein-Barr virus (EBV) expression; MYC translocation status. Results: univariate...
Focal adhesion kinase (FAK) and proline-rich tyrosine 2 (PYK2) are structurally related kinases. They implicated in regulating actin cytoskeleton organization, a process critical for cell migration, mitosis tumor metastasis. In this paper, we demonstrate that, although both PYK2 FAK were expressed colocalized at focal adhesions fibroblasts,microinjection of PYK2, but not FAK, Swiss 3T3 fibroblastic cells led to reorganization rounding. PYK2-mediated required the N terminus, targeting (FAT)...
One of the most fundamental changes in cell morphology is ingression a plasma membrane furrow. The Drosophila embryo undergoes several cycles rapid furrow during early development that culminates formation an epithelial sheet. Previous studies have demonstrated requirement for intracellular trafficking pathways ingression; however, link compartmental behaviors with cortical events remained unclear. Here, we show Rab8 has striking dynamic vivo. As furrows ingress, cytoplasmic puncta are...
Isolated MYC rearrangement without other recurrent genetic abnormalities is rare in B lymphoblastic leukemia/lymphoma (B-ALL/LBL), with most cases reported pediatric patients. We report 3 adult lymphoblasts showing a precursor cell immunophenotype, and isolated /IGH translocation. All occurred male patients initial presentation of diffuse lymphadenopathy. Cases 1 2 had B-ALL significantly increased peripheral blood bone marrow. Case 3, patient human immunodeficiency virus infection, the...
How pulsed contractile dynamics drive the remodeling of cell and tissue topologies in epithelial sheets has been a key question development disease. Due to constraints imaging analysis technologies, studies that have described vivo mechanisms underlying changes neighbor relationships largely confined analyses planar apical regions. Thus, how volumetric nature cells affects force propagation surface three dimensions, including especially apical-basal axis, is unclear. Here, we perform lattice...
In the early Drosophila embryo, germband elongation is driven by oriented cell intercalation through t1 transitions, where vertical (dorsal–ventral aligned) interfaces contract and then resolve into new horizontal (anterior–posterior interfaces. Here, we show that contractile events produce a continuous “rectification” of interfaces, in which systematically rotate toward more orientations. As rotate, their behavior transitions from elongating to regimes, indicating planar polarized...
Axons experience strong mechanical forces due to animal movement. While these serve as sensory cues in mechanosensory neurons, their impact on other neuron types remains poorly defined. Here, we uncover signaling that controls an axonal cytoskeletal response external physiological and plays a key role integrity. Live imaging of microtubules at single-polymer resolution C. elegans motor reveals local oscillatory movements fine-tune polymer positioning. Combining cell-specific chemogenetic...