A5069671066- Parasitic Diseases Research and Treatment
- Parasitic Infections and Diagnostics
- Parasites and Host Interactions
- Amoebic Infections and Treatments
- Toxoplasma gondii Research Studies
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Public Health and Nutrition
- Trypanosoma species research and implications
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Child Nutrition and Water Access
- Parasitic infections in humans and animals
- Rabies epidemiology and control
- Antifungal resistance and susceptibility
- Cancer Treatment and Pharmacology
- Malaria Research and Control
- Herpesvirus Infections and Treatments
- Complement system in diseases
- Nematode management and characterization studies
- Viral Infections and Immunology Research
- Colorectal Cancer Treatments and Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Gastric Cancer Management and Outcomes
University of Indonesia
2014-2024
Eijkman Institute for Molecular Biology
2018
Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo
2008-2018
Oxford University Clinical Research Unit Indonesia
2018
Padjadjaran University
2018
University of Oxford
2018
Imperial College London
1991-1998
University of Edinburgh
1995
Abstract A population of 164 adult individuals resident in an area endemic for Brugia malayi lymphatic filariasis has been studied humoral immune responses to filarial parasites. Antibody levels Ag extracted from worms were determined each the IgG subclasses, IgM and IgE. The dominant isotype antifilarial antibody was IgG4, which represented 88% total asymptomatic microfilaremics, most whom possessed 100 1000 micrograms/ml specific this subclass (geometric mean 762 micrograms/ml). Patients...
ABSTRACT Lymphatic filariasis is a major tropical disease caused by the mosquito-borne nematodes Brugia and Wuchereria . About 120 million people are infected at risk of lymphatic pathology such as acute lymphangitis elephantiasis. Vaccines against must generate immunity to infective mosquito-derived third-stage larva (L3) without accentuating immunopathogenic responses lymphatic-dwelling adult parasites. We have identified two highly expressed genes, designated abundant larval transcript-1...
We investigated the occurrence of intestinal parasites in Indonesian HIV/AIDS patients with chronic diarrhoea prior to administering antiretroviral therapy. The influence age, CD4(+) cell count and season on parasite was also studied. In total, 318 unconcentrated stool samples were analysed using Lugol's iodine modified acid fast staining detect coccidia. Most (94.5%) from males aged 21-40 years counts < or = 50 cells/mm(3). Parasites found 84.3% (single species infections, 71.4%;...
In an area in which brugian filariasis is endemic, when cytokine levels were analyzed as a function of clinical status comparing those who asymptomatic and amicrofilaremic with microfilaremic, it was found that both interferon (IFN)-γ interleukin (IL)-5 suppressed microfilariae carriers (P < .01 P .001, respectively), but IL-4 unabated. Age had significant effect on production groups. subjects, high young persons decreased age, whereas microfilaremic increased significantly age. Conversely,...
Journal Article T Cell Responsiveness Correlates Differentially with Antibody Isotype Levels in Clinical and Asymptomatic Filariasis Get access Maria Yazdanbakhsh, Yazdanbakhsh Reprints or correspondence: Dr. Dept. of Parasito1ogy, Leiden University, Wassenaarseweg 62, Postbus 9605, 2300 RC Leiden, Netherlands. Search for other works by this author on: Oxford Academic PubMed Google Scholar William A. Paxton, Paxton Yvonne C. M. Kruize, Kruize Erliyani Sartono, Sartono Agnes Kurniawan,...
Background: Parasitic gastrointestinal infections have been variably reported among immunocompromised adults while data on children limited. This prospective cross-sectional study aimed to assess the clinical profile of intestinal parasitic with diarrhoea and their treatment response. Methodology: Two freshly voided stool samples taken for two consecutive days were examined by direct formalin-ether concentrated smears. Modified Ziehl-Neelsen staining was used detect Cryptosporidium, Isospora...
IBS is a functional gastrointestinal disorder and commonly presents in children adolescences, presented as diarrhoea, constipation or mixed type. Blastocystis common intestinal protozoa found worldwide, which pathogenicity still controversial. This study aimed to identify the risk factors of IBS, association between types with subtypes analyse pathogenicity.A comparative cross-sectional was conducted among senior high school students. Rome III Criteria for diagnosis, questionnaires on were...
Journal Article Elevated Cellular Immune Responses and Interferon-γ Release after Long-Term Diethylcarbamazine Treatment of Patients with Human Lymphatic Filariasis Get access Erliyani Sartono, Sartono Search for other works by this author on: Oxford Academic PubMed Google Scholar Yvonne C. M. Kruize, Kruize Agnes Kurniawan, Kurniawan Peter H. van der Meide, Meide Felix Partono, Partono Rick Maizels, Maizels Maria Yazdanbakhsh Reprints or correspondence: Dr. Yazdanbakhsh, Dept. Parasitology,...
Understanding the basic immunology of an infectious disease requires insight into pattern T cell reactivity and specificity. Although lymphatic filariasis is a major tropical disease, predominant Ags filarial species such as Brugia malayi are still undefined. We have now identified prominent Ag from B. microfilariae (Mf) Bm-SPN-2, serpin secreted exclusively by this stage. Mf-infected mice mounted strong, but short-lived, Bm-SPN-2-specific Th1 responses, measured in vitro production...
Journal Article Differential Decline in Filaria-Specific IgG1, IgG4, and IgE Antibodies Brugia malayi-Infected Patients after Diethylcarbamazine Chemotherapy Get access Agnes Kurniawan Atmadja, Atmadja Search for other works by this author on: Oxford Academic PubMed Google Scholar Rebecca Atkinson, Atkinson Erliyani Sartono, Sartono Felix Partono, Partono Maria Yazdanbakhsh, Yazdanbakhsh Rick M. Maizels Reprints or correspondence: Dr. R. Maizels, Institute of Cell, Animal Population Biology,...
Through the years, diarrhea has been one of significant causes mortality among children under age five years old all over world. Throughout 2021, approximately 444,000 died due to diarrhea, making up about 9% old’s mortality. Diarrhea is a gastrointestinal disease characterized by increased bowel movements causing three or more stool episodes along with loose watery consistency within 24 hours. There are several risk factors that contribute old. This study aimed determine which enough cause...
Human lymphatic filariasis is a major tropical disease transmitted through mosquito vectors which take up microfilarial larvae from the blood of infected subjects. Microfilariae are produced by long-lived adult parasites, also release suite excretory-secretory products that have recently been subject to in-depth proteomic analysis. Surprisingly, most abundant secreted protein Brugia malayi triose phosphate isomerase (TPI), glycolytic enzyme usually associated with cytosol. We now show while...
Little detailed knowledge is available regarding the etiology and outcome of CNS infection, particularly in HIV-infected individuals, low-resource settings.From January 2015 to April 2016, we prospectively included all adults with suspected infection a referral hospital Jakarta, Indonesia. Systematic screening HIV testing, CSF examination, neuroimaging.A total 274 patients (median age 26 years) presented after median 14 days headache (77%), fever (78%), seizures (27%), or loss consciousness...
Blastocystis hominis is an enteric protozoan with many subtypes. It frequently found in children and may cause chronic diarrhea. This study revealed subtypes among primary school comparison of molecular technique culture method diagnosis. A total 141 stools were collected, examined microscopically, selected into the negative parasite groups, for diagnostic between 18S rRNA polymerase chain reaction (PCR) methods. Positive PCR amplicons subsequently sequenced subtyping. The results 89%, 78%,...
B-cells play a critical role in the formation of immune responses against pathogens by acting as antigen-presenting cells, modulating and generating memory antibody responses. Here, we studied B-cell subset distributions between regions with higher lower microbial exposure, i.e. comparing peripheral blood from people living Indonesia or Ghana to those healthy Dutch residents using 36-marker mass cytometry panel. By applying an unbiased multidimensional approach, observed differences balance...
In an attempt to overcome T cell unresponsiveness filarial antigens, 65 individuals belonging the three clinical groups of elephantiasis patients, microfilaraemics, and asymptomatic amicrofilaraemics who exhibited Brugia malayi adult worm antigen (BmA) were studied. Peripheral blood mononuclear cells cocultured with one following reagents that have been reported be effective in reconstituting proliferation: interleukin-2 (IL-2), interleukin-7 (IL-7), anti-interleukin-4, anti-interleukin-10,...
Abstract Natural infection with filarial nematode parasites results in immune responses skewed towards T helper (Th)2, while mycobacteria shows many characteristics of a Th1‐dominated response. Cytokines typifying Th1, interferon (IFN)‐γ, and Th2, interleukin (IL)‐4, were measured following stimulation peripheral blood mononuclear cells from patients Brugia malayi adult worm antigen (BmA) purified protein derivative Mycobacterium tuberculosis (PPD). In response to PPD, only 1 out 81 produced...
We have isolated and sequenced clones encoding the repeated subunit of surface-associated glycoprotein gp15/400 from two nematode species predominantly responsible for lymphatic filariasis in humans: Brugia malayi Wuchereria bancrofti. The amino acid sequence 15-kDa subunit, derived nucleotide gene fragment B. malayi, is identical to that previously reported pahangi, whereas W. bancrofti protein differs only 7 132 residues. identity allowed us use a recombinant pahangi examine serological...
Immune responses to the intracellular Wolbachia bacteria of filarial nematodes are thought contribute pathologic process infection. Here, we compare antibody subjects living in an area where lymphatic filariasis is endemic with elicited a murine model infection, provide evidence that infective larval stage (L3), not adult nematodes, primary inducer against Wolbachia. In human subjects, Brugia malayi surface protein (WSP) most often correlated L3 B. malayi. Analysis anti-WSP induced mice by...